Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,839,349	C→A	V215V (GTG→GTT)	nlpA ←	lipoprotein‑28

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,839,349	0	C	A	100.0%	200.7 / NA	58	V215V (GTG→GTT)	nlpA	lipoprotein‑28
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (37/21);  total (37/21)

GCTTTAGCGACTTCGGGTGATTGATAAGATTGCAGAAATTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATAT  >  NC_000913/3839218‑3839485                                                                                                                                    |                                                                                                                                         gCTTTAGCGACTTCGGGTGATTGATAAGATTGCAGAAATTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTAGCTTCAATAAATACGCTGTCGTGAACCGgaga                                                                                                                                  <  2:228039/140‑1 (MQ=255)     tAGCGACTTCGGGTGATTGATAAGATTGCAGAAATTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGc                                                                                                                              <  2:713974/140‑1 (MQ=255)       gCGACTTCGGGTGATTGATAAGATTGCAGAAATTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGgag                                                                                                                                   <  1:553924/133‑1 (MQ=255)       gCGACTTCGGGTGATTGATAAGATTGCAGAAATTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGgag                                                                                                                                   >  2:553924/1‑133 (MQ=255)          aCTTCGGGTGATTGATAAGATTGCAGAAATTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGt                                                                                                                         <  2:434579/140‑1 (MQ=255)             tCGGGTGATTGATAAGATTGCAGAAATTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTctg                                                                                                                      >  1:45722/1‑140 (MQ=255)               gggTGATTGATAAGATTGCAGAAATTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTctgct                                                                                                                    <  2:222118/140‑1 (MQ=255)                             aTTGCAGAAATTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAgtggtg                                                                                                      <  2:623078/140‑1 (MQ=255)                              ttGCAGAAATTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGTGAAAGCCCGGTCTGCTGAATGTAAGTGGTGc                                                                                                     >  1:51915/1‑140 (MQ=255)                              ttGCAGAAATTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGc                                                                                                     >  1:636772/1‑140 (MQ=255)                               tGCAGAAATTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCt                                                                                                    >  2:592568/1‑140 (MQ=255)                               tGCAGAAATTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCt                                                                                                    >  2:726327/1‑140 (MQ=255)                               tGCAGAAATTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCt                                                                                                    >  1:464630/1‑140 (MQ=255)                                 cAGAAATTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAg                                                                                                           <  2:704776/131‑1 (MQ=255)                                 cAGAAATTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAg                                                                                                           >  1:704776/1‑131 (MQ=255)                                 cAGAAATTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGa                                                                                                  >  2:517613/1‑140 (MQ=255)                                  aGAAATTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGAt                                                                                                 >  2:469893/1‑140 (MQ=255)                                      aTTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATc                                                                                             >  2:293729/1‑140 (MQ=255)                                      aTTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATc                                                                                             >  2:460596/1‑140 (MQ=255)                                       ttCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCg                                                                                            >  1:1636/1‑140 (MQ=255)                                       ttCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCg                                                                                            <  1:460596/140‑1 (MQ=255)                                             cACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACAt                                                                                      <  2:464630/140‑1 (MQ=255)                                               cGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCa                                                                                    >  2:62664/1‑140 (MQ=255)                                                     cTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTg                                                                              <  1:726327/140‑1 (MQ=255)                                                           tCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGatca                                                                        <  2:45722/140‑1 (MQ=255)                                                                     ttCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTAcgc                                                              >  2:16951/1‑140 (MQ=255)                                                                     ttCCCGTGCCACCAAAAAATTCACATACGGCGAATTCTAATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTAcgc                                                              >  1:218923/1‑140 (MQ=255)                                                                       cccGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTggg                                                                            >  1:332553/1‑124 (MQ=255)                                                                       cccGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTggg                                                                            <  2:332553/124‑1 (MQ=255)                                                                       cccGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTAcgcgc                                                            >  1:494761/1‑140 (MQ=255)                                                                       cccGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTAcgcgc                                                            >  2:207381/1‑140 (MQ=255)                                                                       cccGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTAcgcgc                                                            >  2:647092/1‑140 (MQ=255)                                                                       cccGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATC‑ACTTTGGGATCATCCAGTAcgcgcg                                                           >  2:95017/1‑140 (MQ=255)                                                                             ccaccaAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGc                                                      >  2:723072/1‑140 (MQ=255)                                                                               accaAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATTAACTTTGGGATCATCCAGTACGCGCGGGAGCTg                                                    >  1:35962/1‑140 (MQ=255)                                                                                        ttCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTc                                           <  2:317371/140‑1 (MQ=255)                                                                                          cacaTACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGa                                         <  1:293729/140‑1 (MQ=255)                                                                                               aCGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTcc                                    >  1:191586/1‑140 (MQ=255)                                                                                                cGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCa                                   >  1:419035/1‑140 (MQ=255)                                                                                                cGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCa                                   <  2:218923/140‑1 (MQ=255)                                                                                                  gCGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATa                                 >  1:277451/1‑140 (MQ=255)                                                                                                   cGAATTCTTATCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATaa                                >  2:522184/1‑140 (MQ=255)                                                                                                            aTCTTCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAAt                       >  1:504231/1‑140 (MQ=255)                                                                                                                tCAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACg                   >  2:55555/1‑140 (MQ=255)                                                                                                                 cAATAAATACGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGa                  >  2:89721/1‑140 (MQ=255)                                                                                                                  aataaataCGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAg                 >  2:59833/1‑140 (MQ=255)                                                                                                                  aataaataCGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAg                 >  1:58129/1‑140 (MQ=255)                                                                                                                    taaataCGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGa               >  1:632026/1‑140 (MQ=255)                                                                                                                    taaataCGCTGTCGTGAACCGGAGAAAGACCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGa               >  2:179147/1‑140 (MQ=255)                                                                                                                        taCGCTGTCGTGAACCGGTGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTAt           <  2:51915/140‑1 (MQ=255)                                                                                                                        taCGCTGTCGTGAACCGGAGAAAGCCCGGTCTGGTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGGTCCATAATCTGCAAATGACGAGGATTAt           <  1:180514/140‑1 (MQ=255)                                                                                                                        taCGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTAt           <  1:400916/140‑1 (MQ=255)                                                                                                                        taCGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTAt           <  2:636772/140‑1 (MQ=255)                                                                                                                        taCGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTAt           <  1:207381/140‑1 (MQ=255)                                                                                                                        taCGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTAt           <  1:647092/140‑1 (MQ=255)                                                                                                                         aCGCTGTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATc          >  1:262256/1‑140 (MQ=255)                                                                                                                              gTCGTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTta     >  1:416358/1‑140 (MQ=255)                                                                                                                                 gTGAACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTatat  >  1:623731/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                         GCTTTAGCGACTTCGGGTGATTGATAAGATTGCAGAAATTCCTTCACGTTTTCTGCATTCTTATTATCTTCCCGTGCCACCAAAATATTCACATACGGCGAATTCTTATCTTCAATAAATACGCTGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATAT  >  NC_000913/3839218‑3839485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑