Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,349,010	C→A	intergenic (‑266/+305)	dcuB ← / ← dcuR	anaerobic C4‑dicarboxylate transporter DcuB/DNA‑binding transcriptional activator DcuR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,349,010	0	C	A	100.0%	234.1 / NA	67	intergenic (‑266/+305)	dcuB/dcuR	anaerobic C4‑dicarboxylate transporter DcuB/DNA‑binding transcriptional activator DcuR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (36/31);  total (36/31)

CACAGTGAATATTGGCTGATTAAAGCAGCGGCAAATTTATGCACTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTA  >  NC_000913/4348875‑4349143                                                                                                                                        |                                                                                                                                      cacaGTGAATATTGGCTGATTAAAGCAGCGGCAAATTTATGCACTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTAtattat                                                                                                                                   >  1:63896/1‑140 (MQ=255) cacaGTGAATATTGGCTGATTAAAGCAGCGGCAAATTTATGCACTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTAtattat                                                                                                                                   >  1:509362/1‑140 (MQ=255) cacaGTGAATATTGGCTGATTAAAGCAGCGGCAAATTTATGCACTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTAtattat                                                                                                                                   <  2:376077/140‑1 (MQ=255) cacaGTGAATATTGGCTGATTAAAGCAGCGGCAAATTTATGCACTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTAtattat                                                                                                                                   >  1:281384/1‑140 (MQ=255)   caGTGAATATTGGCTGATTAAAGCAGCGGCAAATTTATGCACTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTg                                                                                                                                 <  2:63896/140‑1 (MQ=255)         atatTGGCTGATTAAAGCAGCGGCAAATTTATGCACTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATAttt                                                                                                                           >  2:679118/1‑140 (MQ=255)          tatTGGCTGATTAAAGCAGCGGCAAATTTATGCACTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTa                                                                                                                          <  2:281384/140‑1 (MQ=255)              ggCTGATTAAAGCAGCTGCAAATTTATGCACTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCgg                                                                                                                      >  1:583687/1‑140 (MQ=255)                cTGATTAAAGCAGCGGCAAATTTATGCACTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTc                                                                                                                    >  1:206406/1‑140 (MQ=255)                cTGATTAAAGCAGCGGCAAATTTATGCACTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTc                                                                                                                    >  1:239086/1‑140 (MQ=255)                  gATTAAAGCAGCGGCAAATTTATGCACTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTa                                                                                                                  >  2:66396/1‑140 (MQ=255)                             cGGCAAATTTATGCACTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTtct                                                                                                       >  2:609212/1‑140 (MQ=255)                             cGGCAAATTTATGCACTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTtct                                                                                                       >  2:177890/1‑140 (MQ=255)                             cGGCAAATTTATGCACTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTtct                                                                                                       <  2:282503/140‑1 (MQ=255)                             cGGCAAATTTATGCACTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTtct                                                                                                       >  1:13601/1‑140 (MQ=255)                                  aaTTTATGCACTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAggg                                                                                                  <  1:70081/140‑1 (MQ=255)                                     ttATGCACTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAg                                                                                               <  1:686134/140‑1 (MQ=255)                                     ttATGCACTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAg                                                                                               <  2:610634/140‑1 (MQ=255)                                     ttATGCACTGTAATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAg                                                                                               <  2:417653/140‑1 (MQ=255)                                            cTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCAt                                                                                        <  2:509362/140‑1 (MQ=255)                                            cTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCAt                                                                                        <  1:679118/140‑1 (MQ=255)                                              gtgATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAg                                                                                               >  2:343586/1‑131 (MQ=255)                                              gtgATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAg                                                                                               <  1:343586/131‑1 (MQ=255)                                               tgATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAg                                                                                     >  2:189665/1‑140 (MQ=255)                                               tgATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAg                                                                                     >  1:277121/1‑140 (MQ=255)                                               tgATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAg                                                                                     <  1:660792/140‑1 (MQ=255)                                                   aaGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGttt                                                                                 <  2:239086/140‑1 (MQ=255)                                                    aGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTta                                                                                <  2:206406/140‑1 (MQ=255)                                                        gCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGa                                                                            <  1:66396/140‑1 (MQ=255)                                                         cTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCAt                                                                                        >  2:363435/1‑127 (MQ=255)                                                         cTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCAt                                                                                        <  1:363435/127‑1 (MQ=255)                                                         cTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGAt                                                                           >  2:242182/1‑140 (MQ=255)                                                         cTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGAt                                                                           >  1:95925/1‑140 (MQ=255)                                                         cTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGAt                                                                           >  1:585533/1‑140 (MQ=255)                                                           ttttCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGa                                                                            <  2:680334/137‑1 (MQ=255)                                                           ttttCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGa                                                                            >  1:680334/1‑137 (MQ=255)                                                           ttttCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTa                                                                         <  1:206605/140‑1 (MQ=255)                                                             ttCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAAt                                                                       <  2:446881/140‑1 (MQ=255)                                                                  agagaAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGAt                                                                  <  2:149015/140‑1 (MQ=255)                                                                     gaAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTa                                                               <  2:536405/140‑1 (MQ=255)                                                                            tctctGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCAttt                                                        >  2:154827/1‑140 (MQ=255)                                                                                tGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTtgtg                                                     <  2:587575/139‑1 (MQ=255)                                                                                tGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTtgtg                                                     >  1:587575/1‑139 (MQ=255)                                                                                   cGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTa                                                 <  2:511744/140‑1 (MQ=255)                                                                                           ttATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATaca                                         <  2:583687/140‑1 (MQ=255)                                                                                           ttATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATaca                                         <  1:609212/140‑1 (MQ=255)                                                                                             ataGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATAcaca                                       <  2:675135/140‑1 (MQ=255)                                                                                                 tttGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAAt                                   <  1:154827/140‑1 (MQ=255)                                                                                                   tGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTTTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGc                                 >  1:489893/1‑140 (MQ=255)                                                                                                   tGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGc                                 >  1:317042/1‑140 (MQ=255)                                                                                                   tGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGc                                 >  2:289299/1‑140 (MQ=255)                                                                                                         gcgcAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGTTCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGa                           >  1:57291/1‑140 (MQ=255)                                                                                                          cgcAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAAt                                   <  2:125340/131‑1 (MQ=255)                                                                                                          cgcAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAAt                                   >  1:125340/1‑131 (MQ=255)                                                                                                          cgcAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGAc                          >  2:588678/1‑140 (MQ=255)                                                                                                          cgcAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGAc                          >  1:367588/1‑140 (MQ=255)                                                                                                          cgcAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGAc                          >  1:15915/1‑140 (MQ=255)                                                                                                          cgcAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGAc                          >  2:93332/1‑140 (MQ=255)                                                                                                          cgcAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGAc                          >  1:560641/1‑140 (MQ=255)                                                                                                          cgcAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGAc                          >  1:661903/1‑140 (MQ=255)                                                                                                           gcAGATGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACa                         >  1:556679/1‑140 (MQ=255)                                                                                                               aTGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACataat                     <  2:95925/140‑1 (MQ=255)                                                                                                               aTGTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACataat                     >  1:445954/1‑140 (MQ=255)                                                                                                                 gTTAATGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAg                   <  2:13601/140‑1 (MQ=255)                                                                                                                    aaTGTTTTGTTTGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGtta                >  1:514581/1‑140 (MQ=255)                                                                                                                      tgttttgtttGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGttaat              <  2:560641/140‑1 (MQ=255)                                                                                                                      tgttttgtttGCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGttaat              <  1:189665/140‑1 (MQ=255)                                                                                                                         tttgtttgCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGttaattaa           >  1:445436/1‑140 (MQ=255)                                                                                                                         tttgtttgCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGttaattaa           >  2:29579/1‑140 (MQ=255)                                                                                                                           tgtttgCTGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACt         >  1:512645/1‑140 (MQ=255)                                                                                                                                  tGTTATATTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTa  >  2:484037/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      CACAGTGAATATTGGCTGATTAAAGCAGCGGCAAATTTATGCACTGTGATAAGCGGCTTTTTCAGAGAGAAAGACTCTCTGGCGATGGGTTTATAGTTTGTACTGCGCAGATGTTAATGTTTTGTTTGCTGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTA  >  NC_000913/4348875‑4349143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑