Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	479,285	G→A	intergenic (‑34/+82)	ylaC ← / ← maa	putative inner membrane protein/maltose O‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	479,285	0	G	A	100.0%	166.6 / NA	47	intergenic (‑34/+82)	ylaC/maa	putative inner membrane protein/maltose O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/28);  total (19/28)

TTACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGCCCACGACAACGTTGTCCGG  >  NC_000913/479151‑479420                                                                                                                                       |                                                                                                                                        ttACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAacac                                                                                                                                    >  2:194389/1‑140 (MQ=255) ttACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAacac                                                                                                                                    <  2:258387/140‑1 (MQ=255) ttACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAacac                                                                                                                                    <  1:33150/140‑1 (MQ=255)      gATAAAACTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTAt                                                                                                                               >  2:476029/1‑140 (MQ=255)       aTAAAACTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTAtt                                                                                                                              >  1:693004/1‑140 (MQ=255)           aaCTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTaa                                                                                                                          <  2:145154/140‑1 (MQ=255)            aCTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAAc                                                                                                                         <  2:228375/140‑1 (MQ=255)             cTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACg                                                                                                                        <  1:39679/140‑1 (MQ=255)              tGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGt                                                                                                                       <  2:237440/140‑1 (MQ=255)                 tACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTggg                                                                                                                    <  2:277177/140‑1 (MQ=255)                      aaGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAAtag                                                                                                               >  1:581494/1‑140 (MQ=255)                         cGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAAtagtag                                                                                                            <  1:63057/140‑1 (MQ=255)                          ggggTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTaga                                                                                                           <  2:676581/140‑1 (MQ=255)                          ggggTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTaga                                                                                                           <  1:98181/140‑1 (MQ=255)                             gTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAggg                                                                                                        <  2:560109/140‑1 (MQ=255)                              tttGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAgggg                                                                                                       >  2:458972/1‑140 (MQ=255)                              tttGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAgggg                                                                                                       <  2:38791/140‑1 (MQ=255)                              tttGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAgggg                                                                                                       <  1:194389/140‑1 (MQ=255)                                              tCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTT‑CAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACca                                                                                      <  1:617099/140‑1 (MQ=255)                                                    gTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAAtt                                                                                 <  1:30070/140‑1 (MQ=255)                                                              aCTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACa                                                                       >  2:466068/1‑140 (MQ=255)                                                              aCTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACa                                                                       >  1:232157/1‑140 (MQ=255)                                                               cTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAg                                                                      <  1:87029/140‑1 (MQ=255)                                                               cTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAg                                                                      >  2:426396/1‑140 (MQ=255)                                                               cTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAg                                                                      <  2:710384/140‑1 (MQ=255)                                                                tCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGt                                                                     >  1:165249/1‑140 (MQ=255)                                                                            gTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCg                                                         >  2:511386/1‑140 (MQ=255)                                                                                       ttGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAAt                                                 <  1:543409/137‑1 (MQ=255)                                                                                       ttGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAAt                                                 >  2:543409/1‑137 (MQ=255)                                                                                                ggTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAattatt                                     <  2:581494/140‑1 (MQ=255)                                                                                                ggTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAattatt                                     >  1:673360/1‑140 (MQ=255)                                                                                                 gTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTc                                    >  2:338933/1‑140 (MQ=255)                                                                                                  tCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTct                                   <  2:232157/140‑1 (MQ=255)                                                                                                  tCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTct                                   <  2:693004/140‑1 (MQ=255)                                                                                                    aTCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTctgg                                 >  1:902/1‑140 (MQ=255)                                                                                                    aTCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTctgg                                 >  1:605670/1‑140 (MQ=255)                                                                                                            ccTTCGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTa                         <  2:673360/140‑1 (MQ=255)                                                                                                                cGTGGTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGc                     >  1:680615/1‑140 (MQ=255)                                                                                                                   ggTATCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGCCCa                  <  1:426396/140‑1 (MQ=255)                                                                                                                      aTCTGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGCCCACGa               <  1:635255/140‑1 (MQ=255)                                                                                                                         tGCAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGCCCACGACaa            >  2:467715/1‑140 (MQ=255)                                                                                                                           cAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGCCCACGACAACg          >  2:287973/1‑140 (MQ=255)                                                                                                                           cAACTTTCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGCCCACGACAACg          <  1:511386/140‑1 (MQ=255)                                                                                                                                ttCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGCCCACGACAACGTTGTc     <  2:902/140‑1 (MQ=255)                                                                                                                                 tCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGCCCACGACAACGTTGt      <  1:236367/138‑1 (MQ=255)                                                                                                                                 tCCAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGCCCACGACAACGTTGt      >  2:236367/1‑138 (MQ=255)                                                                                                                                   cAGGAAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGCCCACGACAACGTTGTCCgg  <  2:680615/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TTACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTCGCGTTTTTCGCGGGTATTCAGAGACTCAATCGTTTCGGTCAGCAGGCGTTGTATTTCGGTCATCGTTTGCCTTCGTGGTATCTGCAACTTTCCAGGGAACACCCTATTTTAACGTGGGAATAGTAGAGGGGAAGTAACAGATCTACCACAATTTTGCATAACAGTTGCGAAAAACCGATTACAATTTTTTAATTATTCTGGCTGGATTACCGCCCACGACAACGTTGTCCGG  >  NC_000913/479151‑479420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑