Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,658,376	G→T	R103L (CGC→CTC)	ynfE →	putative selenate reductase YnfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,658,376	0	G	T	100.0%	260.7 / NA	73	R103L (CGC→CTC)	ynfE	putative selenate reductase YnfE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (41/32);  total (41/32)

GTCAACTGTGGTAGCCGCTGTGCACTTCGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCGCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACTATCGCCAGTAGCCTGAAGAAAACCGTCGAA  >  NC_000913/1658240‑1658512                                                                                                                                         |                                                                                                                                         gTCAACTGTGGTAGCCGCTGTGCACTTCGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATcctccg                                                                                                                                       <  1:511691/140‑1 (MQ=255) gTCAACTGTGGTAGCCGCTGTGCACTTCGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATcctccg                                                                                                                                       >  1:287255/1‑140 (MQ=255) gTCAACTGTGGTAGCCGCTGTGCACTTCGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATcctccg                                                                                                                                       >  2:925241/1‑140 (MQ=255) gTCAACTGTGGTAGCCGCTGTGCACTTCGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCAGTCGCTCCATcctccg                                                                                                                                       <  1:1155226/140‑1 (MQ=255)   cAACTGTGGTAGCCGCTGTGCACTTCGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATcctccg                                                                                                                                       <  2:984371/138‑1 (MQ=255)   cAACTGTGGTAGCCGCTGTGCACTTCGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATcctccg                                                                                                                                       >  1:984371/1‑138 (MQ=255)   cAACTGTGGTAGCCGCTGTGCACTTCGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGc                                                                                                                                     <  1:925241/140‑1 (MQ=255)    aaCTGTGGTAGCCGCTGTGCACTTCGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCg                                                                                                                                    >  2:643908/1‑140 (MQ=255)       tgtgGTAGCCGCTGTGCACTTCGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTAt                                                                                                                                 <  2:670281/140‑1 (MQ=255)         tgGTAGCCGCTGTGCACTTCGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTa                                                                                                                               <  1:1136980/140‑1 (MQ=255)         tgGTAGCCGCTGTGCACTTCGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTa                                                                                                                               >  2:810293/1‑140 (MQ=255)         tgGTAGCCGCTGTGCACTTCGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTa                                                                                                                               >  1:1027945/1‑140 (MQ=255)                   tgtgCACTTCGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGt                                                                                                                                   >  2:378912/1‑126 (MQ=255)                   tgtgCACTTCGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGt                                                                                                                                   <  1:378912/126‑1 (MQ=255)                     tgCACTTCGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATc                                                                                                                   >  2:1260039/1‑140 (MQ=255)                            cGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGaa                                                                                                            <  2:441703/140‑1 (MQ=255)                            cGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGaa                                                                                                            <  1:235847/140‑1 (MQ=255)                               cTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTa                                                                                                         >  1:614528/1‑140 (MQ=255)                                            aTAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAAcgc                                                                                            <  2:1149829/140‑1 (MQ=255)                                            aTAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAAcgc                                                                                            >  2:967750/1‑140 (MQ=255)                                               aTGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTg                                                                                         >  2:488601/1‑140 (MQ=255)                                               aTGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTg                                                                                         >  1:867016/1‑140 (MQ=255)                                               aTGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTg                                                                                         >  1:1049113/1‑140 (MQ=255)                                               aTGAAGTGACCTGGGTAGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTg                                                                                         >  2:1156526/1‑140 (MQ=255)                                                tGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTgg                                                                                        >  2:1003739/1‑140 (MQ=255)                                                tGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTgg                                                                                        >  2:1062649/1‑140 (MQ=255)                                                     tGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCaaa                                                                                   >  1:1072904/1‑140 (MQ=255)                                                      gACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAAc                                                                                  >  1:344554/1‑140 (MQ=255)                                                      gACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAAc                                                                                  >  2:1015079/1‑140 (MQ=255)                                                       aCCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAAcg                                                                                 >  1:1015832/1‑140 (MQ=255)                                                       aCCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAAcg                                                                                 >  1:623141/1‑140 (MQ=255)                                                       aCCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAAcg                                                                                 >  2:695959/1‑140 (MQ=255)                                                        ccTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAAcgc                                                                                >  1:450826/1‑140 (MQ=255)                                                           gggTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACgcggc                                                                             <  2:1086956/140‑1 (MQ=255)                                                               ggAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAg                                                                         >  1:425350/1‑140 (MQ=255)                                                                 aaaCTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGc                                                                       >  2:764159/1‑140 (MQ=255)                                                                  aaCTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCa                                                                      >  1:56957/1‑140 (MQ=255)                                                                   aCTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCaa                                                                     >  1:1200440/1‑140 (MQ=255)                                                                      gACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAAtt                                                                  >  1:177678/1‑140 (MQ=255)                                                                      gACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAAtt                                                                  >  2:746019/1‑140 (MQ=255)                                                                       aCAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTc                                                                 >  1:101091/1‑140 (MQ=255)                                                                       aCAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTc                                                                 >  1:1159787/1‑140 (MQ=255)                                                                        cAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCg                                                                <  2:702361/140‑1 (MQ=255)                                                                        cAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCg                                                                <  2:344554/140‑1 (MQ=255)                                                                          aTACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGaa                                                              <  1:810293/140‑1 (MQ=255)                                                                          aTACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGaa                                                              <  2:201661/140‑1 (MQ=255)                                                                           tACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAAc                                                             >  1:209510/1‑140 (MQ=255)                                                                              cGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGa                                                          >  2:964015/1‑140 (MQ=255)                                                                                   gCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGc                                                     <  2:1200440/140‑1 (MQ=255)                                                                                     gATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTg                                                   >  2:1023233/1‑140 (MQ=255)                                                                                       tGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTggg                                                 >  2:1007721/1‑140 (MQ=255)                                                                                          gTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATg                                              <  2:727640/140‑1 (MQ=255)                                                                                          gTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATg                                              <  1:1066490/140‑1 (MQ=255)                                                                                          gTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATg                                              <  2:257861/140‑1 (MQ=255)                                                                                          gTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATg                                              <  2:287255/140‑1 (MQ=255)                                                                                          gTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATg                                              <  2:390294/140‑1 (MQ=255)                                                                                          gTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATg                                              <  2:425350/140‑1 (MQ=255)                                                                                            aCGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCCAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGaa                                            <  2:913056/140‑1 (MQ=255)                                                                                            aCGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGaa                                            <  2:208024/140‑1 (MQ=255)                                                                                               gCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGcc                                         <  1:1003739/140‑1 (MQ=255)                                                                                               gCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGcc                                         <  2:1027945/140‑1 (MQ=255)                                                                                               gCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGcc                                         <  1:746019/140‑1 (MQ=255)                                                                                               gCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGcc                                         <  1:643908/140‑1 (MQ=255)                                                                                                   ccATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTgg                                     >  2:1279527/1‑140 (MQ=255)                                                                                                     aTCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGAt                                   >  2:1204577/1‑140 (MQ=255)                                                                                                        aGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACt                                <  1:1279527/140‑1 (MQ=255)                                                                                                        aGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACt                                <  2:1015832/140‑1 (MQ=255)                                                                                                          gTACGCGCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACTAt                              <  2:450826/140‑1 (MQ=255)                                                                                                             cgcgCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACTATCGc                           >  1:133215/1‑140 (MQ=255)                                                                                                             cgcgCCTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACTATCGc                           >  2:944255/1‑140 (MQ=255)                                                                                                                 ccTGTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACTATCGCCAGt                       >  2:649712/1‑140 (MQ=255)                                                                                                                    gTTTGCGCGGTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACTATCGCCAGTAGc                    >  2:729601/1‑140 (MQ=255)                                                                                                                            ggTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACTATCGCCAGTAGCCTgaagaa            <  1:1204577/140‑1 (MQ=255)                                                                                                                            ggTCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACTATCGCCAGTAGCCTgaagaa            >  1:213381/1‑140 (MQ=255)                                                                                                                              tCGCTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACTATCGCCAGTAGCCTGAAGaaaa          >  1:57621/1‑140 (MQ=255)                                                                                                                                 cTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATTAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACTATCGCCAGTAGCCTGAAGAAAACCg       >  1:189915/1‑140 (MQ=255)                                                                                                                                 cTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACTATCGCCAGTAGCCTGAAGAAAACCg       <  2:867016/140‑1 (MQ=255)                                                                                                                                 cTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACTATCGCCAGTAGCCTGAAGAAAACCg       <  1:764159/140‑1 (MQ=255)                                                                                                                                 cTCCATCCTCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACTATCGCCAGTAGCCTGAAGAAAACCg       <  1:1062649/140‑1 (MQ=255)                                                                                                                                      tcctccGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACTATCGCCAGTAGCCTGAAGAAAACCGTCGaa  <  2:133215/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GTCAACTGTGGTAGCCGCTGTGCACTTCGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACTGACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCGCCGGCGTATTAATCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACTATCGCCAGTAGCCTGAAGAAAACCGTCGAA  >  NC_000913/1658240‑1658512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑