BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,755,649	G→T	noncoding (57/363 nt)	ssrA →	tmRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,755,649	0	G	T	98.2%	400.6 / ‑1.1	111	noncoding (57/363 nt)	ssrA	tmRNA
Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (58/51); total (59/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

TGCTAAATCTTTAACGATAACGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAA  >  NC_000913/2755517‑2755783

tGCTAAATCTTTAACGATAACGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGcc                                                                                                                                 <  2:1149202/140‑1 (MQ=255)

tGCTAAATCTTTAACGATAACGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGcc                                                                                                                                 >  1:371955/1‑140 (MQ=255)

tGCTAAATCTTTAACGATAACGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGcc                                                                                                                                 >  2:967824/1‑140 (MQ=255)

          ttAACGATAACGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAg                                                                                                                       >  2:568013/1‑140 (MQ=255)

            aaCGATAACGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGcc                                                                                                                     >  1:390027/1‑140 (MQ=255)

                  aaCGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCTGAGGGGCTGTTGGCCTCGTAAAAAGCCGCaaaa                                                                                                               >  1:893707/1‑140 (MQ=255)

                   aCGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCaaaaa                                                                                                              >  2:815884/1‑140 (MQ=255)

                    cGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCaaaaaa                                                                                                             >  1:32382/1‑140 (MQ=255)

                    cGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACA‑TGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAAt                                                                                                            >  1:738790/1‑140 (MQ=255)

                         ttGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGt                                                                                                         <  1:1025036/139‑1 (MQ=255)

                         ttGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGt                                                                                                         >  2:1025036/1‑139 (MQ=255)

                         ttGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTc                                                                                                        >  1:768899/1‑140 (MQ=255)

                                 ggTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAacga                                                                                                >  1:120174/1‑140 (MQ=255)

                                 ggTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAacga                                                                                                >  2:1263649/1‑140 (MQ=255)

                                 ggTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAacga                                                                                                >  2:1055649/1‑140 (MQ=255)

                                 ggTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAacga                                                                                                >  2:34296/1‑140 (MQ=255)

                                 ggTCATGGCGCTCATAAATCTGGAATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAacga                                                                                                >  2:1255622/1‑140 (MQ=255)

                                        gcgcTCATAAATCTGGTATACTTACCTTTACACATTGGTGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAAc                                                                                         <  2:893707/140‑1 (MQ=255)

                                        gcgcTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAAc                                                                                         <  1:398313/140‑1 (MQ=255)

                                        gcgcTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAAc                                                                                         <  1:621879/140‑1 (MQ=255)

                                            tCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACg                                                                                     >  2:1183665/1‑140 (MQ=255)

                                            tCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACg                                                                                     >  1:422475/1‑140 (MQ=255)

                                            tCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACg                                                                                     >  1:1052297/1‑140 (MQ=255)

                                            tCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACg                                                                                     >  2:111738/1‑140 (MQ=255)

                                                  aTCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTag                                                                               <  2:422475/140‑1 (MQ=255)

                                                    cTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTagca                                                                             <  2:371955/140‑1 (MQ=255)

                                                    cTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTagca                                                                             <  1:107197/140‑1 (MQ=255)

                                                    cTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTagca                                                                             <  1:977388/140‑1 (MQ=255)

                                                        tataCTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTagca                                                                             >  2:1168679/1‑136 (MQ=255)

                                                        tataCTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTagca                                                                             <  1:1168679/136‑1 (MQ=255)

                                                          taCTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTtaa                                                                       >  1:140347/1‑140 (MQ=255)

                                                             ttACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTtaataa                                                                    >  2:1282163/1‑140 (MQ=255)

                                                              tACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAAc                                                                   >  2:667857/1‑140 (MQ=255)

                                                               aCCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAAcc                                                                  >  2:991813/1‑140 (MQ=255)

                                                               aCCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAAcc                                                                  >  1:795567/1‑140 (MQ=255)

                                                               aCCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTtaataa                                                                    >  2:1243510/1‑138 (MQ=255)

                                                               aCCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTtaataa                                                                    <  1:1243510/138‑1 (MQ=255)

                                                                 cTTTACACATTGGGTCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAATCGACGAAAACTACGCTTTagc                                                                              <  2:204870/126‑1 (MQ=255)

                                                                 cTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTagc                                                                              >  1:204870/1‑126 (MQ=255)

                                                                 cTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTg                                                                >  2:593910/1‑140 (MQ=255)

                                                                   ttACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCt                                                              <  1:34296/140‑1 (MQ=255)

                                                                    tACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCtt                                                             >  1:436035/1‑140 (MQ=255)

                                                                    tACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCtt                                                             <  2:245918/140‑1 (MQ=255)

                                                                    tACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCtt                                                             >  2:7152/1‑140 (MQ=255)

                                                                    tACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCtt                                                             >  1:362324/1‑140 (MQ=255)

                                                                     acacATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTa                                                            >  1:814364/1‑140 (MQ=255)

                                                                      cacaTTGGGCCTGATCCTGGATTCGACCGGATTTGCCAAACCCAAGCGGCATGCCGAGGTGCTGCTGGCCTTGTAAAACGCTACAAAAAATAGTCGCAAACGCCGAAATCTCCGCTTTAGAAGCTTAATAATCTGCTTag                                                           >  2:443895/1‑140 (MQ=255)

                                                                         aTTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTaga                                                          <  1:537129/138‑1 (MQ=255)

                                                                         aTTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTaga                                                          >  2:537129/1‑138 (MQ=255)

                                                                         aTTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGc                                                        <  2:870322/140‑1 (MQ=255)

                                                                           tGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGccc                                                      >  2:89378/1‑140 (MQ=255)

                                                                           tGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGccc                                                      >  1:254963/1‑140 (MQ=255)

                                                                           tGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGccc                                                      >  2:1244757/1‑140 (MQ=255)

                                                                           tGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGccc                                                      >  1:843292/1‑140 (MQ=255)

                                                                             gggCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCctc                                                    <  1:89378/140‑1 (MQ=255)

                                                                             gggCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCctc                                                    <  1:1263649/140‑1 (MQ=255)

                                                                             gggCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCctc                                                    <  1:815884/140‑1 (MQ=255)

                                                                              ggCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCctct                                                   >  1:362754/1‑140 (MQ=255)

                                                                               gCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCctctc                                                  >  1:1050838/1‑140 (MQ=255)

                                                                                  gATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCct                                                     <  1:1219082/134‑1 (MQ=255)

                                                                                  gATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCct                                                     >  2:1219082/1‑134 (MQ=255)

                                                                                   aTTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCATAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCctc                                                    <  2:334312/134‑1 (MQ=255)

                                                                                    ttCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCt                                             <  2:658185/140‑1 (MQ=255)

                                                                                     tCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTaga                                                          >  2:516185/1‑126 (MQ=255)

                                                                                     tCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTaga                                                          <  1:516185/126‑1 (MQ=255)

                                                                                        ggATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGcc                                         >  2:44369/1‑140 (MQ=255)

                                                                                          aTTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTc                                       <  2:1140558/140‑1 (MQ=255)

                                                                                          aTTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTc                                       <  1:568013/140‑1 (MQ=255)

                                                                                          aTTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTc                                       <  2:795567/140‑1 (MQ=255)

                                                                                          aTTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTc                                       <  1:967824/140‑1 (MQ=255)

                                                                                          aTTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTc                                       <  1:999157/140‑1 (MQ=255)

                                                                                           ttCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTcc                                      <  2:738790/140‑1 (MQ=255)

                                                                                            tCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCg                                     <  2:120174/140‑1 (MQ=255)

                                                                                                   gATTTGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAg                              >  1:1169439/1‑140 (MQ=255)

                                                                                                     tttGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGa                            <  2:254963/140‑1 (MQ=255)

                                                                                                       tGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACg                          <  2:768899/140‑1 (MQ=255)

                                                                                                       tGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACg                          <  2:814364/140‑1 (MQ=255)

                                                                                                       tGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACg                          <  1:1183665/140‑1 (MQ=255)

                                                                                                       tGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACg                          <  1:1189052/140‑1 (MQ=255)

                                                                                                       tGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACg                          <  1:1055649/140‑1 (MQ=255)

                                                                                                       tGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACg                          <  2:1169439/140‑1 (MQ=255)

                                                                                                       tGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACg                          <  2:1052297/140‑1 (MQ=255)

                                                                                                       tGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACg                          <  2:412591/140‑1 (MQ=255)

                                                                                                       tGCGAAACCCAAGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACg                          <  1:44369/140‑1 (MQ=255)

                                                                                                                 aaGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAaga                <  1:500556/140‑1 (MQ=255)

                                                                                                                  aGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAagag               <  1:111738/140‑1 (MQ=255)

                                                                                                                  aGGTGCATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAagag               >  1:1142571/1‑140 (MQ=255)

                                                                                                                       cATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTc          <  2:140347/140‑1 (MQ=255)

                                                                                                                       cATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTc          <  1:667857/140‑1 (MQ=255)

                                                                                                                       cATGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTc          <  1:36101/140‑1 (MQ=255)

                                                                                                                        aTGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCa         >  1:888248/1‑140 (MQ=255)

                                                                                                                         tGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACg                          <  1:135091/122‑1 (MQ=255)

                                                                                                                         tGCCGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCaa        >  2:43253/1‑140 (MQ=255)

                                                                                                                           ccGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAAc      <  1:801988/140‑1 (MQ=255)

                                                                                                                           ccGAGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAAc      <  1:1282163/140‑1 (MQ=255)

                                                                                                                              aGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCa   >  1:195623/1‑140 (MQ=255)

                                                                                                                              aGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCa   >  1:1087437/1‑140 (MQ=255)

                                                                                                                              aGGGGCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCa   >  1:1071254/1‑140 (MQ=255)

                                                                                                                               ggggCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCaa  >  2:700817/1‑140 (MQ=255)

                                                                                                                               ggggCTGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCaa  >  2:1278862/1‑140 (MQ=255)

TGCTAAATCTTTAACGATAACGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAA  >  NC_000913/2755517‑2755783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`