Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,281,333	A→C	K452Q (AAA→CAA)	yjcE →	putative transporter YjcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,281,333	0	A	C	100.0%	299.4 / NA	76	K452Q (AAA→CAA)	yjcE	putative transporter YjcE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (35/41);  total (35/41)

TCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAG  >  NC_000913/4281201‑4281467                                                                                                                                     |                                                                                                                                       tcGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATgga                                                                                                                                 >  1:1035946/1‑140 (MQ=255)  cGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATggag                                                                                                                                <  1:923027/140‑1 (MQ=255)  cGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATggag                                                                                                                                <  1:357082/140‑1 (MQ=255)           tCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTg                                                                                                                       <  1:828835/140‑1 (MQ=255)            cGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTgg                                                                                                                      <  2:1225430/140‑1 (MQ=255)              gCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTggcg                                                                                                                    <  1:1227932/140‑1 (MQ=255)                   gtgATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTggcggc                                                                                                                  <  2:1206060/137‑1 (MQ=255)                   gtgATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTggcggc                                                                                                                  >  1:1206060/1‑137 (MQ=255)                         ttGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTggcggc                                                                                                                  >  2:737056/1‑131 (MQ=255)                         ttGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTggcggc                                                                                                                  <  1:737056/131‑1 (MQ=255)                         ttGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGa                                                                                                         >  1:57715/1‑140 (MQ=255)                         ttGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGa                                                                                                         <  1:405749/140‑1 (MQ=255)                           gCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGaa                                                                                                        <  2:828250/139‑1 (MQ=255)                           gCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGaa                                                                                                        >  1:828250/1‑139 (MQ=255)                           gCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAag                                                                                                       >  2:326267/1‑140 (MQ=255)                            ccTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAaga                                                                                                      >  2:1084941/1‑140 (MQ=255)                               aTTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGaa                                                                                                   <  1:766270/140‑1 (MQ=255)                               aTTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGaa                                                                                                   <  2:1269130/140‑1 (MQ=255)                               aTTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGaa                                                                                                   <  2:808102/140‑1 (MQ=255)                                 tCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAata                                                                                                 >  1:211624/1‑140 (MQ=255)                                    gCTACAACACATTGAAGTCGCGGCTCATTCGCAACAATTGAACGAGGAACGAATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGAGGCGATCCTGCAAACGGTGGCGCGCCTGGCGGCGGGTACCGAAGTGCATACCg                                                                                              >  2:1042738/1‑140 (MQ=255)                                    gCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCg                                                                                              >  2:767435/1‑140 (MQ=255)                                     cTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGaa                                                                                                   >  1:356137/1‑134 (MQ=255)                                     cTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGaa                                                                                                   <  2:356137/134‑1 (MQ=255)                                     cTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGa                                                                                             >  2:186555/1‑140 (MQ=255)                                     cTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGa                                                                                             >  1:53531/1‑140 (MQ=255)                                      tACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGAt                                                                                            >  1:842998/1‑140 (MQ=255)                                          acacATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAAcc                                                                                        >  2:786668/1‑140 (MQ=255)                                           cacaTTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCa                                                                                       >  2:936117/1‑140 (MQ=255)                                           cacaTTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCa                                                                                       >  2:904846/1‑140 (MQ=255)                                              aTTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAgct                                                                                    <  2:1140102/140‑1 (MQ=255)                                              aTTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAgct                                                                                    <  2:57715/140‑1 (MQ=255)                                               ttGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAgctg                                                                                   >  1:1282825/1‑140 (MQ=255)                                               ttGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAgctg                                                                                   >  2:241519/1‑140 (MQ=255)                                                tGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAgctgc                                                                                  >  2:1265341/1‑140 (MQ=255)                                                tGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATACAGCAAGTGGAGAAGCGGCTAGCGGCGGATACCGAAGAGAATATCGATAACCAgctgc                                                                                  >  2:1158936/1‑140 (MQ=255)                                                   aGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTa                                                                               <  2:672732/140‑1 (MQ=255)                                                    gTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTAc                                                                              <  2:211624/140‑1 (MQ=255)                                                      cgcgGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACgg                                                                            >  2:989343/1‑140 (MQ=255)                                                                 cGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTtct                                                                 <  2:53531/140‑1 (MQ=255)                                                                      caaTTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGt                                                            <  2:1153829/140‑1 (MQ=255)                                                                      caaTTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGt                                                            <  1:36524/140‑1 (MQ=255)                                                                      caaTTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGt                                                            <  2:722114/140‑1 (MQ=255)                                                                      caaTTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGt                                                            <  2:842998/140‑1 (MQ=255)                                                                      caaTTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGt                                                            <  1:837863/140‑1 (MQ=255)                                                                      caaTTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGt                                                            <  1:489261/140‑1 (MQ=255)                                                                                agGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAAc                                                  >  1:1159875/1‑140 (MQ=255)                                                                                 ggAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAAcc                                                 >  2:880992/1‑140 (MQ=255)                                                                                  gAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCt                                                >  2:1127935/1‑140 (MQ=255)                                                                                   aaCGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGTGCGGCTGGGGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTCACGGAGGTCAGTTCTCGCGTCATTAGTAACCTg                                               >  2:1209846/1‑140 (MQ=255)                                                                                   aaCGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGTGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGt                                                            >  2:315862/1‑127 (MQ=255)                                                                                   aaCGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGTGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGt                                                            <  1:315862/127‑1 (MQ=255)                                                                                   aaCGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTg                                                       >  1:102062/1‑132 (MQ=255)                                                                                   aaCGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTg                                                       <  2:102062/132‑1 (MQ=255)                                                                                   aaCGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTg                                               >  1:1084755/1‑140 (MQ=255)                                                                                          tGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCgtcgtc                                        >  2:996466/1‑140 (MQ=255)                                                                                          tGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCgtcgtc                                        >  1:1167814/1‑140 (MQ=255)                                                                                                aGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCg                                  <  2:821099/140‑1 (MQ=255)                                                                                                 gCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGa                                 >  1:929619/1‑140 (MQ=255)                                                                                                 gCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGa                                 <  2:1167814/140‑1 (MQ=255)                                                                                                  cGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGAt                                <  2:1165732/140‑1 (MQ=255)                                                                                                        cGGCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGt                          <  1:996466/140‑1 (MQ=255)                                                                                                         ggCAGAAGTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTa                         <  2:813994/140‑1 (MQ=255)                                                                                                                gTGGCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGt                  >  1:1212210/1‑140 (MQ=255)                                                                                                                   gCGATTGTGGCGATCCAGCAAATGGAGTAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGa               >  1:835066/1‑140 (MQ=255)                                                                                                                   gCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGa               <  2:1035946/140‑1 (MQ=255)                                                                                                                   gCGATTGTGGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGa               >  1:647833/1‑140 (MQ=255)                                                                                                                        tgtgGCGATCCAGCAAATGGAGTAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGtt          <  2:835066/140‑1 (MQ=255)                                                                                                                         gtgGCGATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTc         <  1:1196636/140‑1 (MQ=255)                                                                                                                              gATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGGTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGTCGTAACGACGTTGAAAGTTCCGTGc    <  2:1282825/140‑1 (MQ=255)                                                                                                                              gATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGc    <  1:1265341/140‑1 (MQ=255)                                                                                                                              gATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGc    <  2:1159875/140‑1 (MQ=255)                                                                                                                              gATCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGc    <  1:904846/140‑1 (MQ=255)                                                                                                                                tCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAg  <  1:326267/140‑1 (MQ=255)                                                                                                                                tCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAg  <  1:936117/140‑1 (MQ=255)                                                                                                                                tCCAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAg  <  1:989343/140‑1 (MQ=255)                                                                                                                                 ccAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCa   >  1:1092582/1‑138 (MQ=255)                                                                                                                                 ccAGCAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCa   <  2:1092582/138‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       TCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAG  >  NC_000913/4281201‑4281467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑