BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,539,822	C→A	intergenic (‑321/‑1135)	nanC ← / → fimB	N‑acetylneuraminic acid outer membrane channel/regulator for fimA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,539,822	0	C	A	100.0%	535.0 / NA	154	intergenic (‑321/‑1135)	nanC/fimB	N‑acetylneuraminic acid outer membrane channel/regulator for fimA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (81/73); total (81/73)

Only 100 of 155 total aligned reads displayed.

ATCCAGATTAAATACCTGAGTATAAAATCTCTTCTGATGTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTCCTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTTATACCTGTTATACCAGATCAAAAATCACG  >  NC_000913/4539687‑4539957

   cAGATTAAATACCTGAGTATAAAATCTCTTCTGATGTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAg                                                                                                                                  <  2:583811/140‑1 (MQ=255)

      aTTAAATACCTGAGTATAAAATCTCTTCTGATGTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACt                                                                                                                               >  1:277113/1‑140 (MQ=255)

          aaTACCTGAGTATAAAATCTCTTCTGATGTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAg                                                                                                                           >  2:1212891/1‑140 (MQ=255)

                tGAGTATAAAATCTCTTCTGATGTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGtt                                                                                                                     >  2:1137080/1‑140 (MQ=255)

                 gAGTATAAAATCTCTTCTGATGTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGttt                                                                                                                    >  1:412559/1‑140 (MQ=255)

                 gAGTATAAAATCTCTTCTGATGTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGttt                                                                                                                    >  1:1162946/1‑140 (MQ=255)

                  aGTATAAAATCTCTTCTGATGTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGtttt                                                                                                                   >  2:1277012/1‑140 (MQ=255)

                  aGTATAAAATCTCTTCTGATGTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGtttt                                                                                                                   >  1:260094/1‑140 (MQ=255)

                    tataAAATCTCTTCTGATGTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTtca                                                                                                                 >  2:866176/1‑140 (MQ=255)

                         aaTCTCTTCTGATGTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCCCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCAcc                                                                                                            <  2:133787/140‑1 (MQ=255)

                            ctctTCTGATGTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTtcat                                                                                                                >  1:342092/1‑133 (MQ=255)

                            ctctTCTGATGTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTtcat                                                                                                                <  2:342092/133‑1 (MQ=255)

                            ctctTCTGATGTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGa                                                                                                         >  2:1151288/1‑140 (MQ=255)

                              cttctGATGTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATg                                                                                                       >  1:799881/1‑140 (MQ=255)

                              cttctGATGTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATg                                                                                                       >  1:652480/1‑140 (MQ=255)

                              cttctGATGTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATg                                                                                                       >  2:440516/1‑140 (MQ=255)

                              cttctGATGTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATg                                                                                                       >  1:106880/1‑140 (MQ=255)

                              cttctGATGTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATg                                                                                                       <  2:799881/140‑1 (MQ=255)

                                      gTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGagaaagaa                                                                                               >  2:285814/1‑140 (MQ=255)

                                                 ttGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATg                                                                                    >  1:727032/1‑140 (MQ=255)

                                                   gAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGtt                                                                                  <  2:427406/140‑1 (MQ=255)

                                                        ttCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCa                                                                             >  2:83489/1‑140 (MQ=255)

                                                         tCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCAt                                                                            >  2:980607/1‑140 (MQ=255)

                                                              aGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGAtt                                                                       >  1:969496/1‑140 (MQ=255)

                                                              aGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGAtt                                                                       <  2:106880/140‑1 (MQ=255)

                                                                  atatCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCga                                                                   >  2:89608/1‑140 (MQ=255)

                                                                  atatCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCga                                                                   >  1:174600/1‑140 (MQ=255)

                                                                   tatCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCgag                                                                  >  1:25293/1‑140 (MQ=255)

                                                                    atCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCgaga                                                                 >  1:475662/1‑140 (MQ=255)

                                                                     tCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGAt                                                                <  1:842110/140‑1 (MQ=255)

                                                                     tCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGAt                                                                >  2:393845/1‑140 (MQ=255)

                                                                          tACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGcc                                                             >  2:168701/1‑138 (MQ=255)

                                                                          tACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCt                                                           >  1:145600/1‑140 (MQ=255)

                                                                           aCTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGcc                                                             >  1:107675/1‑137 (MQ=255)

                                                                           aCTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTg                                                          >  2:7379/1‑140 (MQ=255)

                                                                             tCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAATAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGtc                                                        >  1:517988/1‑140 (MQ=255)

                                                                             tCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGcc                                                             <  1:890248/135‑1 (MQ=255)

                                                                             tCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGcc                                                             >  2:890248/1‑135 (MQ=255)

                                                                             tCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGtc                                                        >  1:665238/1‑140 (MQ=255)

                                                                             tCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGtc                                                        >  1:684609/1‑140 (MQ=255)

                                                                             tCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGtc                                                        >  1:692663/1‑140 (MQ=255)

                                                                             tCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGtc                                                        >  1:1272179/1‑140 (MQ=255)

                                                                             tCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGtc                                                        <  2:1072889/140‑1 (MQ=255)

                                                                               aaTCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGtctc                                                      >  2:40919/1‑140 (MQ=255)

                                                                               aaTCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGtctc                                                      <  1:281240/140‑1 (MQ=255)

                                                                               aaTCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGtctc                                                      >  1:34124/1‑140 (MQ=255)

                                                                               aaTCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGtctc                                                      >  2:274139/1‑140 (MQ=255)

                                                                                 tCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCAt                                                    >  1:967215/1‑140 (MQ=255)

                                                                                  ccATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCAtt                                                   <  2:1165623/140‑1 (MQ=255)

                                                                                    aTTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGa                                                 <  1:393845/140‑1 (MQ=255)

                                                                                     ttttACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGtctc                                                      <  2:1000794/134‑1 (MQ=255)

                                                                                     ttttACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGtctc                                                      >  1:1000794/1‑134 (MQ=255)

                                                                                       ttACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTa                                              <  2:937745/140‑1 (MQ=255)

                                                                                        tACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTAt                                             <  1:274139/140‑1 (MQ=255)

                                                                                               agTCATAAAATAGAGTGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTg                                      <  1:874749/140‑1 (MQ=255)

                                                                                               agTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGa                                                 <  2:1033243/129‑1 (MQ=255)

                                                                                               agTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGa                                                 >  1:1033243/1‑129 (MQ=255)

                                                                                               agTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTg                                      <  2:231788/140‑1 (MQ=255)

                                                                                               agTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTg                                      <  2:25293/140‑1 (MQ=255)

                                                                                               agTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTg                                      >  2:28381/1‑140 (MQ=255)

                                                                                               agTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTg                                      <  2:34124/140‑1 (MQ=255)

                                                                                               agTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTg                                      >  1:1010934/1‑140 (MQ=255)

                                                                                               agTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTg                                      <  2:444199/140‑1 (MQ=255)

                                                                                               agTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTg                                      <  2:856744/140‑1 (MQ=255)

                                                                                               agTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTg                                      >  2:108151/1‑140 (MQ=255)

                                                                                               agTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTg                                      <  1:1043937/140‑1 (MQ=255)

                                                                                               agTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTg                                      <  1:1311340/140‑1 (MQ=255)

                                                                                               agTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTg                                      <  1:800522/140‑1 (MQ=255)

                                                                                               agTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTg                                      <  1:83489/140‑1 (MQ=255)

                                                                                               agTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTg                                      <  1:861273/140‑1 (MQ=255)

                                                                                                gTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGc                                     <  1:911385/140‑1 (MQ=255)

                                                                                                gTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGc                                     <  1:526527/140‑1 (MQ=255)

                                                                                                  cATAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCAc                                   >  2:678653/1‑140 (MQ=255)

                                                                                                   aTAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCAcc                                  >  2:519706/1‑140 (MQ=255)

                                                                                                    tAAAATAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCt                                 >  1:1154853/1‑140 (MQ=255)

                                                                                                         tAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTtata                            <  2:1272179/140‑1 (MQ=255)

                                                                                                         tAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTtata                            <  1:866176/140‑1 (MQ=255)

                                                                                                         tAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTtata                            <  1:1137080/140‑1 (MQ=255)

                                                                                                         tAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTtata                            <  1:124265/140‑1 (MQ=255)

                                                                                                         tAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTtata                            <  1:285814/140‑1 (MQ=255)

                                                                                                         tAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTtata                            <  1:39481/140‑1 (MQ=255)

                                                                                                         tAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTtata                            <  1:40919/140‑1 (MQ=255)

                                                                                                         tAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTtata                            <  1:440516/140‑1 (MQ=255)

                                                                                                         tAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTtata                            >  1:740393/1‑140 (MQ=255)

                                                                                                         tAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTtata                            <  1:983380/140‑1 (MQ=255)

                                                                                                         tAGAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTtata                            <  2:174600/140‑1 (MQ=255)

                                                                                                         tAGAATGTCTGTTGAGCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCTCCTTtata                            <  2:277113/140‑1 (MQ=255)

                                                                                                           gAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTtat                             <  1:439536/137‑1 (MQ=255)

                                                                                                           gAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTTATAcc                          >  2:603010/1‑140 (MQ=255)

                                                                                                           gAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTTATAcc                          >  1:851381/1‑140 (MQ=255)

                                                                                                           gAATGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTTATAcc                          >  2:919308/1‑140 (MQ=255)

                                                                                                            aaTGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTtata                            <  2:523508/137‑1 (MQ=255)

                                                                                                            aaTGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTtata                            >  1:523508/1‑137 (MQ=255)

                                                                                                              tGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTtat                             >  2:719426/1‑134 (MQ=255)

                                                                                                              tGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTtat                             <  1:719426/134‑1 (MQ=255)

                                                                                                              tGTCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTtat                             >  1:849135/1‑134 (MQ=255)

                                                                                                                tCTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTtat                             >  2:868694/1‑132 (MQ=255)

                                                                                                                 cTGTTGATCTCATTTTCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTTATACCTGTtat                    >  1:460974/1‑140 (MQ=255)

                                                                                                                              tttCATATTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTTATACCTGTTATACCAGATCaaaaa       <  2:851381/140‑1 (MQ=255)

                                                                                                                                   tatTACTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTTATACCTGTTATACCAGATCAAAAATCACg  <  2:1010934/140‑1 (MQ=255)

ATCCAGATTAAATACCTGAGTATAAAATCTCTTCTGATGTTTAATTGATTTGAATGTTCGTAAGCTATATCACTTACTCAATCCATTTTACCCAGAGTCATAAAATAGAATGTCTGTTGATCTCATTTTCATATTCCTTTCAGACTGAAGTGCGTTTTCATCACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTTATACCTGTTATACCAGATCAAAAATCACG  >  NC_000913/4539687‑4539957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`