Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,052,503	G→T	S287* (TCA→TAA)	yccM ←	putative 4Fe‑4S membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,052,503	0	G	T	100.0%	218.0 / NA	62	S287* (TCA→TAA)	yccM	putative 4Fe‑4S membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (31/31);  total (31/31)

ATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATACAACTTTCGGCATTGCGACGAATCTTAA  >  NC_000913/1052371‑1052638                                                                                                                                     |                                                                                                                                        aTTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTAc                                                                                                                                  <  1:1088847/140‑1 (MQ=255)   tGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGc                                                                                                                                >  2:984093/1‑140 (MQ=255)   tGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGc                                                                                                                                >  2:675249/1‑140 (MQ=255)          ccAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCAt                                                                                                                         <  1:1004885/140‑1 (MQ=255)             aTCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACa                                                                                                                      >  2:53902/1‑140 (MQ=255)               cACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGc                                                                                                                    <  2:1191579/140‑1 (MQ=255)                  cGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCgg                                                                                                                 >  1:1047831/1‑140 (MQ=255)                  cGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCgg                                                                                                                 >  1:607683/1‑140 (MQ=255)                   gCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGt                                                                                                                <  1:136415/140‑1 (MQ=255)                   gCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGt                                                                                                                <  2:174477/140‑1 (MQ=255)                   gCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGt                                                                                                                <  1:9935/140‑1 (MQ=255)                    cAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTa                                                                                                               >  1:932182/1‑140 (MQ=255)                     aaaCATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTAc                                                                                                              >  1:916695/1‑140 (MQ=255)                      aaCATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACa                                                                                                             <  1:282219/140‑1 (MQ=255)                      aaCATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACa                                                                                                             <  1:675249/140‑1 (MQ=255)                           aaTCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCa                                                                                                        <  1:923187/140‑1 (MQ=255)                            aTCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCAc                                                                                                       >  2:254651/1‑140 (MQ=255)                                  aGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCg                                                                                                 >  2:600431/1‑140 (MQ=255)                                  aGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCg                                                                                                 <  2:958994/140‑1 (MQ=255)                                   gAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGt                                                                                                >  2:461559/1‑140 (MQ=255)                                   gAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGt                                                                                                >  2:964748/1‑140 (MQ=255)                                     aCCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTAc                                                                                              <  2:932182/140‑1 (MQ=255)                                         gTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGa                                                                                          >  1:390344/1‑140 (MQ=255)                                             cGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGaatta                                                                                      >  2:1282367/1‑140 (MQ=255)                                             cGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGaatta                                                                                      >  1:234025/1‑140 (MQ=255)                                                 aTTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAAttt                                                                                  >  2:113559/1‑140 (MQ=255)                                                    aaCCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATc                                                                               >  2:1006657/1‑140 (MQ=255)                                                      ccagccagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGa                                                                             <  1:426248/140‑1 (MQ=255)                                                       cagccagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGAc                                                                            >  1:365351/1‑140 (MQ=255)                                                       cagccagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGAc                                                                            <  1:1299506/140‑1 (MQ=255)                                                        agccagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGAcc                                                                           <  1:113559/140‑1 (MQ=255)                                                        agccagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGAcc                                                                           <  1:703195/140‑1 (MQ=255)                                                        agccagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGAcc                                                                           <  2:1047831/140‑1 (MQ=255)                                                         gccagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCg                                                                          >  1:1114165/1‑140 (MQ=255)                                                         gccagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCg                                                                          >  2:750269/1‑140 (MQ=255)                                                         gccagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCg                                                                          >  2:1122933/1‑140 (MQ=255)                                                          ccagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCgg                                                                         >  1:571997/1‑140 (MQ=255)                                                          ccagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCgg                                                                         >  1:944342/1‑140 (MQ=255)                                                           cagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCggg                                                                        >  2:658275/1‑140 (MQ=255)                                                             gACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGAt                                                                      <  1:254651/140‑1 (MQ=255)                                                                  cGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTg                                                                 <  2:650965/140‑1 (MQ=255)                                                                     aaaGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATg                                                              <  1:53902/140‑1 (MQ=255)                                                                                tATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAAtttttt                                                   <  2:607683/140‑1 (MQ=255)                                                                                  tttGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTgc                                                 <  1:984093/140‑1 (MQ=255)                                                                                   ttGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTgcg                                                >  1:329935/1‑140 (MQ=255)                                                                                    tGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTgcgc                                               <  1:461559/140‑1 (MQ=255)                                                                                             tttttGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCcac                                      <  2:1114165/140‑1 (MQ=255)                                                                                              ttttGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCcaca                                     <  1:1282367/140‑1 (MQ=255)                                                                                              ttttGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCcaca                                     <  2:944342/140‑1 (MQ=255)                                                                                                ttGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGt                                   <  2:571997/140‑1 (MQ=255)                                                                                                     gTGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATa                              <  1:750269/140‑1 (MQ=255)                                                                                                      tGAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATAc                             >  2:977219/1‑140 (MQ=255)                                                                                                       gAAAAGGTCAATGTTGAGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATACa                            <  1:1152790/140‑1 (MQ=255)                                                                                                                       aGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATACAACTTTCGGCATTGcga            >  2:326171/1‑140 (MQ=255)                                                                                                                       aGGCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATACAACTTTCGGCATTGcga            >  2:1211877/1‑140 (MQ=255)                                                                                                                        ggCTACCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAATCGATACAACTTTCGGCATTGcgac           <  2:390344/140‑1 (MQ=255)                                                                                                                            aCCGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATACAACTTTCGGCATTGCGACGAAt       >  2:507410/1‑140 (MQ=255)                                                                                                                              cGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATACAACTTTCGGCATTGCGACGAATCt     >  2:273193/1‑140 (MQ=255)                                                                                                                              cGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATACAACTTTCGGCATTGCGACGAATCt     >  1:968527/1‑140 (MQ=255)                                                                                                                              cGGACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATACAACTTTCGGCATTGCGACGAATCt     >  1:1310428/1‑140 (MQ=255)                                                                                                                                gACATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATACAACTTTCGGCATTGCGACGAATCTTa   <  1:1282585/140‑1 (MQ=255)                                                                                                                                 aCATTACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATACAACTTTCGGCATTGCGACGAATCTTaa  <  1:600431/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        ATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATACAACTTTCGGCATTGCGACGAATCTTAA  >  NC_000913/1052371‑1052638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑