Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,543,443	C→T	S206S (TCG→TCA)	narU ←	nitrate/nitrite transporter NarU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,543,443	0	C	T	100.0%	72.9 / NA	22	S206S (TCG→TCA)	narU	nitrate/nitrite transporter NarU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/9);  total (13/9)

GGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC  >  NC_000913/1543332‑1543577                                                                                                                |                                                                                                                                       ggCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGa                                                                                                            >  2:490394/1‑140 (MQ=255)         aGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCgaga                                                                                                    <  2:82596/140‑1 (MQ=255)         aGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCgaga                                                                                                    <  1:214550/140‑1 (MQ=255)         aGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCgaga                                                                                                    <  1:490394/140‑1 (MQ=255)                    cTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATAc                                                                                         <  2:212537/140‑1 (MQ=255)                         gATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTa                                                                                    <  1:52849/140‑1 (MQ=255)                            atCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACaa                                                                                 >  1:387395/1‑140 (MQ=255)                                     cccTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCa                                                                        >  1:222095/1‑140 (MQ=255)                                          aCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGt                                                                   >  1:151873/1‑140 (MQ=255)                                               gcggcgATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGAc                                                                          <  1:74961/128‑1 (MQ=255)                                               gcggcgATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGAc                                                                          >  2:74961/1‑128 (MQ=255)                                               gcggcgATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAAc                                                              >  1:435234/1‑140 (MQ=255)                                               gcggcgATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAAc                                                              >  1:179645/1‑140 (MQ=255)                                               gcggcgATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTACAAc                                                              >  1:488082/1‑140 (MQ=255)                                                  gcgATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAg                                                           <  2:442116/140‑1 (MQ=255)                                                  gcgATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTACAACCAg                                                           <  2:488082/140‑1 (MQ=255)                                                      tCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGc                                                       >  2:409495/1‑140 (MQ=255)                                                                     gTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCt                                        >  1:118808/1‑140 (MQ=255)                                                                                               ttCGCCAGCGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATccc              >  2:495542/1‑140 (MQ=255)                                                                                                      gCGACATCCCTGAAGCGTCGGCCTGTGGTACGCCATTTACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAgcg       <  2:179645/140‑1 (MQ=255)                                                                                                       cGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCa                      <  2:361/124‑1 (MQ=255)                                                                                                       cGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCa                      >  1:361/1‑124 (MQ=255)                                                                                                       cGACATCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAgcgc      >  1:111139/1‑140 (MQ=255)                                                                                                           aTCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACTCTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTccc  <  2:435234/140‑1 (MQ=255)                                                                                                           aTCACTGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTccc  <  2:387395/140‑1 (MQ=255)                                                                                                                |                                                                                                                                       GGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC  >  NC_000913/1543332‑1543577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑