Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,613,807	A→T	D299E (GAT→GAA)	sad ←	NAD(+)‑dependent succinate semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,613,807	0	A	T	100.0%	80.5 / NA	24	D299E (GAT→GAA)	sad	NAD(+)‑dependent succinate semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (12/12);  total (12/12)

CCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACGTCCGGCTACCGCCG  >  NC_000913/1613677‑1613940                                                                                                                                   |                                                                                                                                      ccccAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCAtt                                                                                                                              >  2:68156/1‑140 (MQ=255) ccccAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCAtt                                                                                                                              >  1:318301/1‑140 (MQ=255) ccccAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCAtt                                                                                                                              >  2:267754/1‑140 (MQ=255)           cTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCATTTTCAAGGCTg                                                                                                                    >  1:333552/1‑140 (MQ=255)                                                  ggTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGt                                                                                           >  1:285356/1‑126 (MQ=255)                                                  ggTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGt                                                                                           <  2:285356/126‑1 (MQ=255)                                                       tctcCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTccc                                                                        >  1:480306/1‑140 (MQ=255)                                                                 atgatgCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGataa                                                              >  2:323261/1‑140 (MQ=255)                                                                        aGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCg                                                       >  2:96387/1‑140 (MQ=255)                                                                              tcaCGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGc                                                 <  2:318301/140‑1 (MQ=255)                                                                                aCGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCg                                               <  2:333552/140‑1 (MQ=255)                                                                                    aaaTCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCg                                               <  1:114930/136‑1 (MQ=255)                                                                                    aaaTCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCg                                               >  2:114930/1‑136 (MQ=255)                                                                                     aaTCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGca                                          <  1:154893/140‑1 (MQ=255)                                                                                       tCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGcaca                                        >  1:226237/1‑140 (MQ=255)                                                                                            aCGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCt                                   >  2:340064/1‑140 (MQ=255)                                                                                               aGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTc                                <  2:436315/140‑1 (MQ=255)                                                                                                gCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTcc                               <  1:340064/140‑1 (MQ=255)                                                                                                 ccATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCg                              <  1:96387/140‑1 (MQ=255)                                                                                                 ccATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCg                              <  1:267754/140‑1 (MQ=255)                                                                                                 ccATTGGTCCGAGAGCGTTCTCTCCGTCACGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCg                              <  1:110184/140‑1 (MQ=255)                                                                                                    ttGGTCCGAGAGCGTTCTCTTCGTCACGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTa                           <  2:226237/140‑1 (MQ=255)                                                                                                           gagaGCGTTCTCTTCGTCACGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGAt                    <  1:68156/140‑1 (MQ=255)                                                                                                                       ttCGTCACGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACGTCCGGCTa        >  1:272756/1‑140 (MQ=255)                                                                                                                             aCGGGGTTCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACGTCCGGCTAccgccg  <  1:323261/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                      CCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACGTCCGGCTACCGCCG  >  NC_000913/1613677‑1613940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑