Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,920,035	G→T	T842T (ACG→ACT)	yebT →	intermembrane transport protein YebT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,920,035	0	G	T	100.0%	90.0 / NA	26	T842T (ACG→ACT)	yebT	intermembrane transport protein YebT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/13);  total (13/13)

CTATCAACACCTGGTGCGTAACAATTCCGTCTTCTGGTTGGCATCGGGTTACAGTCTGGACTTTGGTCTGACGGGCGGCGTAGTGAAAACCGGCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACGCCTCCGGGTACGCCACTGGCACCGAAAGCCCAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGC  >  NC_000913/1919900‑1920169                                                                                                                                        |                                                                                                                                       cTATCAACACCTGGTGCGTAACAATTCCGTCTTCTGGTTGGCATCGGGTTACAGTCTGGACTTTGGTCTGACGGGCGGCGTAGTGAAAACCGGCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCActcctc                                                                                                                                    <  1:23510/140‑1 (MQ=255)         acCTGGTGCGTAACAATTCCGTCTTCTGGTTGGCATCGGGTTACAGTCTGGACTTTGGTCTGACGGGCGGCGTAGTGAAAACCGGCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGTACg                                                                                                                            >  2:197779/1‑140 (MQ=255)               tGCGTAACAATTCCGTCTTCTGGTTGGCATCGGGTTACAGTCTGGACTTTGGTCTGACGGGCGGCGTAGTGAAAACCGGCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGTACGCCACTg                                                                                                                      <  2:338485/140‑1 (MQ=255)                   tAACAATTCCGTCTTCTGGTTGGCATCGGGTTACAGTCTGGACTTTGGTCTGACGGGCGGCGTAGTGAAAACCGGCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGTACGCCACTGGCAc                                                                                                                  >  2:254873/1‑140 (MQ=255)                     aCAATTCCGTCTTCTGGTTGGCATCGGGTTACAGTCTGGACTTTGGTCTGACGGGCGGCGTAGTGAAAACCGGCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGTACGCCACTGGCACCg                                                                                                                <  1:254873/140‑1 (MQ=255)                         ttCCGTCTTCTGGTTGGCATCGGGTTACAGTCTGGACTTTGGTCTGACGGGCGGCGTAGTGAAAACCGGCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGTACGCCACTGGCACCGAAAg                                                                                                            >  2:340746/1‑140 (MQ=255)                              tcttctGGTTGGCATCGGGTTACAGTCTGGACTTTGGTCTGACGGGCGGCGTAGTGAAAACCGGCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGTACGCCa                                                                                                                         <  2:179366/122‑1 (MQ=255)                              tcttctGGTTGGCATCGGGTTACAGTCTGGACTTTGGTCTGACGGGCGGCGTAGTGAAAACCGGCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGTACGCCa                                                                                                                         >  1:179366/1‑122 (MQ=255)                                 tctGGTTGGCATCGGGTTACAGTCTGGACTTTGGTCTGACGGGCGGCGTAGTGAAAACCGGCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGTACGCCACTGGCACCGAAAGCCCAGGaa                                                                                                    >  2:386029/1‑140 (MQ=255)                                          cATCGGGTTACAGTCTGGACTTTGGTCTGACGGGCGGCGTAGTGAAAACCGGCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGTACGCCACTGGCACCGAAAGCCCAGGAAGGCAAacac                                                                                           >  1:421697/1‑140 (MQ=255)                                                          ggACTTTGGTCTGACGGGCGGCGTAGTGAAAACCGGCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGTACGCCACTGGCACCGAAAGCCCAGGAAGGCAAACACTTCCTGTTGCAGGaaa                                                                           <  1:36119/140‑1 (MQ=255)                                                           gACTTTGGTCTGACGGGCGGCGTAGTGAAAACCGGCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGTACGCCACTGGCACCGAAAGCCCAGGAAGGCAAACACTTCCTGTTGCAGGAAAg                                                                          <  2:421697/140‑1 (MQ=255)                                                            aCTTTGGTCTGACGGGCGGCGTAGTGAAAACCGGCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGTACGCCACTGGCACCGAAAGCCCAGGAAGGCAAACACTTCCTGTTGCAGGAAAGt                                                                         >  2:143567/1‑140 (MQ=255)                                                                 ggTCTGACGGGCGGCGTAGTGAAAACCGGCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGCACGCCACTGGCACCGAAAGCCCAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAAcc                                                                    >  2:311519/1‑140 (MQ=255)                                                                       aCGGGCGGCGTAGTGAAAACCGGCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGTACGCCACTGGCACCGAAAGCCCAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAaga                                                              <  1:197779/140‑1 (MQ=255)                                                                              gcgTAGTGAAAACCGGCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGTACGCCACTGGCACCGAAAGCCCAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGt                                                       <  1:340746/140‑1 (MQ=255)                                                                                 tAGTGAAAACCGGCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGTACGCCACTGGCACCGAAAGCCCAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAAc                                                                     <  1:334873/123‑1 (MQ=255)                                                                                 tAGTGAAAACCGGCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGTACGCCACTGGCACCGAAAGCCCAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAAc                                                                     >  2:334873/1‑123 (MQ=255)                                                                                             gCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGTACGCCACTGGCACCGAAAGCCCAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTgc                                         >  1:367199/1‑139 (MQ=255)                                                                                             gCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGTACGCCACTGGCACCGAAAGCCCAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTgc                                         <  2:367199/139‑1 (MQ=255)                                                                                              cACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGTACGCCACTGGCACCGAAAGCCCAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTgcgc                                       <  1:386029/140‑1 (MQ=255)                                                                                                           tttATCCGTGGCGGCATCGCCTTCGCCACTCCTCCGGGTACGCCACTGGCACCGAAAGCCCAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATg                          >  1:334743/1‑140 (MQ=255)                                                                                                                   tGGCGGCATCGCCTTCGCCACTCCTCCGGGTACGCCACTGGCACCGAAAGCCCAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGc                  <  2:334743/140‑1 (MQ=255)                                                                                                                     gcggcATCGCCTTCGCCACTCCTCCGGGTACGCCACTGGCACCGAAAGCCCAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGcc                        <  2:242048/132‑1 (MQ=255)                                                                                                                     gcggcATCGCCTTCGCCACTCCTCCGGGTACGCCACTGGCACCGAAAGCCCAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGcc                        >  1:242048/1‑132 (MQ=255)                                                                                                                                ttCGCCACTCCTCCGGGTACGCCACTGGCACCGAAAGCCCAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTg     >  1:302463/1‑140 (MQ=255)                                                                                                                                   gCCACTCCTCCGGGTACGCCACTGGCACCGAAAGCCCAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTgcgc  <  2:302463/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       CTATCAACACCTGGTGCGTAACAATTCCGTCTTCTGGTTGGCATCGGGTTACAGTCTGGACTTTGGTCTGACGGGCGGCGTAGTGAAAACCGGCACCTTTAACCAGTTTATCCGTGGCGGCATCGCCTTCGCCACGCCTCCGGGTACGCCACTGGCACCGAAAGCCCAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGC  >  NC_000913/1919900‑1920169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑