Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	197,742	C→A	P399P (CCC→CCA)	rseP →	intramembrane zinc metalloprotease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	197,742	0	C	A	100.0%	108.3 / NA	32	P399P (CCC→CCA)	rseP	intramembrane zinc metalloprotease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/14);  total (18/14)

AAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCA  >  NC_000913/197605‑197871                                                                                                                                          |                                                                                                                                  aaaCTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGt                                                                                                                                 >  2:92550/1‑140 (MQ=255) aaaCTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGt                                                                                                                                 >  2:238408/1‑140 (MQ=255)            ccTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACgggg                                                                                                                      >  1:477759/1‑140 (MQ=255)               cAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTCCCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGc                                                                                                                   <  2:459804/140‑1 (MQ=255)               cAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGc                                                                                                                   >  1:328279/1‑140 (MQ=255)                      ccGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATctgct                                                                                                            >  1:109093/1‑140 (MQ=255)                      ccGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATctgct                                                                                                            >  2:91774/1‑140 (MQ=255)                       cGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATctgctg                                                                                                           <  2:109093/140‑1 (MQ=255)                              tATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTg                                                                                                    >  2:74046/1‑140 (MQ=255)                               aTCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGc                                                                                                                   >  1:118737/1‑124 (MQ=255)                               aTCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGc                                                                                                                   <  2:118737/124‑1 (MQ=255)                                      aGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGaa                                                                                            <  1:74046/140‑1 (MQ=255)                                      aGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGaa                                                                                            <  1:91774/140‑1 (MQ=255)                                        ggggCTGGGATCACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGaa                                                                                            <  1:126312/138‑1 (MQ=255)                                        ggggCTGGGATCACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGaa                                                                                            >  2:126312/1‑138 (MQ=255)                                           gCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGAt                                                                                       <  2:477759/140‑1 (MQ=255)                                            cTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATc                                                                                      >  1:438778/1‑140 (MQ=255)                                                       gCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGAcc                                                                           <  2:464809/140‑1 (MQ=255)                                                                       tttATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGtt                                                           >  2:469310/1‑140 (MQ=255)                                                                         tattaCCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCa                                                         <  1:238408/140‑1 (MQ=255)                                                                                      ttCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTAt                                            >  2:307805/1‑140 (MQ=255)                                                                                       tCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATc                                           >  2:53579/1‑140 (MQ=255)                                                                                           gcgcTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCAt                                       <  2:36136/140‑1 (MQ=255)                                                                                               ttattaGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGc                                   >  1:415944/1‑140 (MQ=255)                                                                                                   taGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGa                               <  1:53579/140‑1 (MQ=255)                                                                                                      cGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTTCTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGTCTTTTGTTATCGCATTGGCTCGATTc                            <  2:81961/140‑1 (MQ=255)                                                                                                           aCTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTctgctg                       >  1:371069/1‑140 (MQ=255)                                                                                                            cTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTgg                      <  1:462574/140‑1 (MQ=255)                                                                                                               aGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGc                   >  1:208724/1‑140 (MQ=255)                                                                                                                      aTTAACCTGTTTCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAAt            <  2:328279/140‑1 (MQ=255)                                                                                                                            cTGTTTCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCt      >  1:59475/1‑140 (MQ=255)                                                                                                                               tttCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGc   >  2:94490/1‑140 (MQ=255)                                                                                                                                ttCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCa  >  1:304847/1‑140 (MQ=255)                                                                                                                                ttCCGTTGCCAGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCa  <  1:469310/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                  AAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCA  >  NC_000913/197605‑197871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑