Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,236,974	G→T	L260L (CTC→CTA)	mglC ←	D‑galactose/methyl‑galactoside ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,236,974	0	G	T	100.0%	67.3 / NA	20	L260L (CTC→CTA)	mglC	D‑galactose/methyl‑galactoside ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/7);  total (13/7)

TATACGTCAGGCCATAGTTGATGACGGTAAAAATAATTACCCCGGTCACCACGCCAATCACCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTA  >  NC_000913/2236838‑2237109                                                                                                                                         |                                                                                                                                        tataCGTCAGGCCATAGTTGATGACGGTAAAAATAATTACCCCGGTCACCACGCCAATCACCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCTAgg                                                                                                                                      >  1:103836/1‑140 (MQ=255) tataCGTCAGGCCAAAGTTGATGACGATAAAAATAATTCCCCCGGTCACCCCCCCAACCACCGTCCCCCCCCCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCTAgg                                                                                                                                      <  2:446386/140‑1 (MQ=255)          ggCCATAGTTGATGACGGTAAAAATAATTACCCCGGTCACCACGCCAATCACCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCTAGGTTGTtggtg                                                                                                                             >  1:296407/1‑140 (MQ=255)           gCCATAGTTGATGACGGTAAAAATAATTCCCCCGGTCCCCCCGCCAATCCCCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCTAGGTTGTtggtgg                                                                                                                            <  1:122011/140‑1 (MQ=255)           gCCATAGTTGATGACGGTAAAAATAATTACCCCGGTCACCACGCCAATCACCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCTAGGTTGTtggtgg                                                                                                                            >  1:249909/1‑140 (MQ=255)           gCCATAGTTGATGACGGTAAAAATAATTACCCCGGTCACCACGCCAATCACCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCTAGGTTGTtggtgg                                                                                                                            >  1:446386/1‑140 (MQ=255)           gCCATAGTTGATGACGGTAAAAATAATTACCCCGGTCACCACGCCAATCACCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCAATAGCATCCAGCTCATACATAAAGCCTAGGTTGTtggtgg                                                                                                                            >  1:171503/1‑140 (MQ=255)                     atgaCGGTAAAAATAATTACCCCGGTCACCACGCCAATCACCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCTAGGTTGTTGGTGGCAGAGCCGAt                                                                                                                  >  1:218351/1‑140 (MQ=255)                              aaaaTAATTCCCCCGGTCCCCACCCCAATCCCCGTCCCCACCCCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCTAGGTTGTTGGTGGCAGAGCCGATACGTCCGGc                                                                                                         <  2:261143/140‑1 (MQ=255)                                               caccacGCCAATCACCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCTAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCaa                                                                                        <  1:141613/140‑1 (MQ=255)                                                    cGCCAACCCCCGCCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCTAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCa                                                                                   <  2:249909/140‑1 (MQ=255)                                                              cGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCTAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGc                                                                         >  2:257517/1‑140 (MQ=255)                                                                  ccccCACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATAGCAACCAGCTCATACATAAAGCCTAGGATGTTGGTGTCTGAGCCAATCCGTCCCGCTTTTAACATCCCGGCAAAGGCATAGAACACAACAGa                                                                     <  2:438613/140‑1 (MQ=255)                                                                    cacaCCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCTAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACa                                                                   <  2:218351/140‑1 (MQ=255)                                                                           ccgcTGAACGATACACCGCCTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCTAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTa                                                            >  1:10315/1‑140 (MQ=255)                                                                            cgcTGAACGATACACCGCCTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCTAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAg                                                           >  1:85350/1‑140 (MQ=255)                                                                            cgcTGAACGATACACCGCCTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCTAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAg                                                           >  2:299730/1‑140 (MQ=255)                                                                                             ccTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCTAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAg                                          >  1:442192/1‑140 (MQ=255)                                                                                                aCCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCTAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGcc                                       <  2:350510/140‑1 (MQ=255)                                                                                                                                  taAAGCCTAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCggg     >  1:163598/1‑140 (MQ=255)                                                                                                                                     aGCCTAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTa  >  2:188315/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TATACGTCAGGCCATAGTTGATGACGGTAAAAATAATTACCCCGGTCACCACGCCAATCACCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTA  >  NC_000913/2236838‑2237109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑