Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,339,206	T→C	D72G (GAC→GGC)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,339,206	0	T	C	100.0%	90.8 / NA	25	D72G (GAC→GGC)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/13);  total (12/13)

CCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGC  >  NC_000913/2339069‑2339329                                                                                                                                          |                                                                                                                            ccgcAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGCCa                                                                                                                           >  2:475512/1‑140 (MQ=255)              gTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGCCACCAACGACACggg                                                                                                              >  2:279585/1‑140 (MQ=255)              agcGATAGAACCGAAGTTCCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGCCACCAACGACACggg                                                                                                              <  1:346895/138‑1 (MQ=255)                         cGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGCCACCAACGACACGGGCAGATTTTTta                                                                                                   >  2:315843/1‑140 (MQ=255)                          gAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGCCACCAACGACCCGGGCAGATTTTTtat                                                                                                  <  2:435905/140‑1 (MQ=255)                           aaGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCGGCGCCATGCGGACGAGCGTGTCATAGTCCGCCGAGTCAACATGGGGATGGTATGTACCGATTACGCCCCCAACCACACGGGCAGATTTTTtact                                                                                                 >  2:346895/1‑138 (MQ=255)                                aCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGCCACCAACGACACGGGCAGATTTTTTATAGGCtt                                                                                            >  1:46638/1‑140 (MQ=255)                                 cccTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGCCACCAACGACACGGGCAGATTTTTTATAGGCttt                                                                                           >  1:72623/1‑140 (MQ=255)                                    tGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGCCACCAACGACACGGGCAGATTTTTTATAGGCTTTGtt                                                                                        <  2:223641/140‑1 (MQ=255)                                           cTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGCCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCa                                                                                 <  1:279585/140‑1 (MQ=255)                                              ccAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGCCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTg                                                                              <  2:141576/140‑1 (MQ=255)                                                            aGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGCCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCa                                                                                 <  1:437088/123‑1 (MQ=255)                                                            aGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGCCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGcc                                                                            <  2:161485/128‑1 (MQ=255)                                                            aGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGCCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGcc                                                                            >  1:161485/1‑128 (MQ=255)                                                            aGCGAGAATGGCTGCGACATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGCCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCa                                                                                 >  2:437088/1‑123 (MQ=255)                                                                          cgcCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTATGCCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTAcgc                                                  >  1:429815/1‑140 (MQ=255)                                                                          cgcCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGCCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTAcgc                                                  >  1:350031/1‑140 (MQ=255)                                                                            ccATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGCCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGa                                                <  1:475512/140‑1 (MQ=255)                                                                                    aCGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGCCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTAc                                        <  2:46638/140‑1 (MQ=255)                                                                                    aCGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGCCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTAc                                        <  1:315843/140‑1 (MQ=255)                                                                                              cATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTATGCCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAgg                              <  2:429815/140‑1 (MQ=255)                                                                                                     cgccgAGTCACCATGGGGATGGTATTTACCGATTACGCCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCAtctc                       <  2:487743/140‑1 (MQ=255)                                                                                                                          gTATTTACCGATTACGCCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGc  >  2:410785/1‑140 (MQ=255)                                                                                                                          gTATTTACCGATTACGCCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGc  >  2:295429/1‑140 (MQ=255)                                                                                                                                     ttACGCCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACgg   <  2:238891/128‑1 (MQ=255)                                                                                                                                     ttACGCCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACgg   >  1:238891/1‑128 (MQ=255)                                                                                                                                          |                                                                                                                            CCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGC  >  NC_000913/2339069‑2339329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑