Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,206,738	G→T	E92D (GAG→GAT)	ttdA →	L(+)‑tartrate dehydratase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,738	0	G	T	100.0%	87.3 / NA	26	E92D (GAG→GAT)	ttdA	L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/14);  total (12/14)

ATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGT  >  NC_000913/3206604‑3206863                                                                                                                                       |                                                                                                                              aTGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGATCTGCa                                                                                                                          <  2:493675/140‑1 (MQ=255)    gggAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGATCTGCAAAg                                                                                                                       <  1:179592/140‑1 (MQ=255)               cTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGATCTGCAAAGCATACTCAAAc                                                                                                            <  2:38830/140‑1 (MQ=255)               cTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGATCTGCAAAGCATACTCAAAc                                                                                                            <  2:373834/140‑1 (MQ=255)                            ttCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAaga                                                                                               >  2:107100/1‑140 (MQ=255)                            ttCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAaga                                                                                               >  1:28688/1‑140 (MQ=255)                                    caTGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCg                                                                                       >  1:465636/1‑140 (MQ=255)                                             aGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAgcgc                                                                              <  1:107100/140‑1 (MQ=255)                                             aGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAgcgc                                                                              <  2:465636/140‑1 (MQ=255)                                               cGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCa                                                                            <  1:319848/140‑1 (MQ=255)                                                  ttGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTa                                                                         <  2:28688/140‑1 (MQ=255)                                                   tGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTAc                                                                        >  2:150981/1‑140 (MQ=255)                                                                           ggACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTg                                                <  1:225009/140‑1 (MQ=255)                                                                               acCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGa                                            >  1:106669/1‑140 (MQ=255)                                                                                 cGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGAAg                                          >  2:130984/1‑140 (MQ=255)                                                                                  ggggAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGAAGt                                         >  1:134940/1‑140 (MQ=255)                                                                                      agaTTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGAAGTAAac                                     >  2:201071/1‑140 (MQ=255)                                                                                         ttATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGAAGTAAACAcc                                  <  1:201071/140‑1 (MQ=255)                                                                                                   cGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATa                        >  1:278338/1‑140 (MQ=255)                                                                                                      tAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCg                     >  2:345865/1‑140 (MQ=255)                                                                                                               ttCCCGCTTCCCACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAgcggcg            <  2:201298/140‑1 (MQ=255)                                                                                                                     cTTCCCACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGt      >  1:221476/1‑140 (MQ=255)                                                                                                                        cccACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTggg   <  2:134940/140‑1 (MQ=255)                                                                                                                         ccACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGt  <  1:345865/140‑1 (MQ=255)                                                                                                                         ccACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGt  <  2:221476/140‑1 (MQ=255)                                                                                                                         ccACTGCTTGGCGATCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGt  >  1:138207/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                              ATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGT  >  NC_000913/3206604‑3206863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑