Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,306,166	C→A	P565P (CCG→CCT)	deaD ←	ATP‑dependent RNA helicase DeaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,306,166	0	C	A	100.0%	41.7 / NA	14	P565P (CCG→CCT)	deaD	ATP‑dependent RNA helicase DeaD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/4);  total (10/4)

TTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAACCACGACCACCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAACAGCTTGATGTTA  >  NC_000913/3306085‑3306301                                                                                  |                                                                                                                                        ttCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAACCACGACCACCGCCACGACGCTCACCGCCAGTATGAGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGATAATGCGAGTGcgcg                                                                               >  1:44581/1‑140 (MQ=255) ttCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAACCACGACCACCGCCACGACGCTCACCGCCAGTATGAGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGcgcg                                                                               >  2:119285/1‑140 (MQ=255)         tGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAACCACGACCACCGCCACGACGCTCACCGCCAGTATGAGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAgtgt                                                                       >  2:470211/1‑140 (MQ=255)                       ccTTCACGACGTTCGCCACCGAAACCACGACCACCGCCACGACGCTCACCGCCAGTATGAGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGCGCGCGTAAAGTGTTGCAGCACTTCAcc                                                         >  1:325663/1‑140 (MQ=255)                                  ttCGCCACCGAAACCACGACCACCGCCACGACGCTCACCGCCAGTATGAGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCtt                                              >  1:62971/1‑140 (MQ=255)                                  ttCGCCACCGAAACCACGACCACCGCCACGACGCTCACCGCCAGTATGAGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTCGCAGCACTTCACCCGGCATACCtt                                              >  2:24505/1‑140 (MQ=255)                                  ttCGCCACCGAAACCACGACCACCGCCACGACGCTCACCGCCAGTATGAGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAAAGCGAGTGCCCGTAAAGGGTTGCAGCACTTCACCCGGCATACCtt                                              >  2:482502/1‑140 (MQ=255)                                                cACGCCCACCGCCACGACGCTCACCGCCAGTATGAGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGAtg                                <  1:354806/140‑1 (MQ=255)                                                   gACCACCGCCACGACGCTCACCGCCAGTATGAGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGAtggtg                             >  1:150828/1‑140 (MQ=255)                                                                acgCTCACCGCCAGTATGAGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCaa                <  1:482502/140‑1 (MQ=255)                                                                     cACCGCCAGTATGAGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAACAGc           <  2:62971/140‑1 (MQ=255)                                                                           cAGTATGAGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCAATGGTGGAGTGAGAAGCAAACAGCTTGATg     <  2:325663/140‑1 (MQ=255)                                                                           cAGTATGAGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGGGCGCGTAAAGTGTTTCAGCACTTCAACCGGCATACCTTTCGGCACGTCGATGGGGGGGTGAGAAGCAAAAAGCTTGATg     >  2:193640/1‑140 (MQ=255)                                                                              tATGAGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAACAGCTTGATGTTa  >  1:107945/1‑140 (MQ=255)                                                                                  |                                                                                                                                        TTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAACCACGACCACCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAACAGCTTGATGTTA  >  NC_000913/3306085‑3306301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑