Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	238,189	T→G	intergenic (+69/+68)	yafT → / ← ykfM	lipoprotein YafT/uncharacterized protein YkfM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	238,189	0	T	G	100.0%	109.0 / NA	29	intergenic (+69/+68)	yafT/ykfM	lipoprotein YafT/uncharacterized protein YkfM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (13/16);  total (13/16)

AAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGCGAGAAATATATCCATTATTACATTTCATGCC  >  NC_000913/238053‑238313                                                                                                                                         |                                                                                                                             aaaTCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGa                                                                                                                           >  2:225919/1‑140 (MQ=255)     cATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACaaa                                                                                                                       >  1:382688/1‑140 (MQ=255)     cATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACaaa                                                                                                                       >  1:51555/1‑140 (MQ=255)       tGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGa                                                                                                                     <  1:225919/140‑1 (MQ=255)       tGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGa                                                                                                                     <  2:382688/140‑1 (MQ=255)       tGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGa                                                                                                                     <  2:468539/140‑1 (MQ=255)                      aGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGa                                                                                                      >  1:283580/1‑140 (MQ=255)                            ataAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGa                                                                                                <  2:12378/140‑1 (MQ=255)                                 gCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCg                                                                                           <  2:36876/140‑1 (MQ=255)                                   gATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGa                                                                                         >  2:223632/1‑140 (MQ=255)                                           aaGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTaaaaa                                                                                 >  1:477592/1‑140 (MQ=255)                                               tttGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACtgt                                                                             <  2:477592/140‑1 (MQ=255)                                                 tGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACtgttt                                                                           >  1:292479/1‑140 (MQ=255)                                                       aCCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTa                                                                     <  1:120627/140‑1 (MQ=255)                                                             aTCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAatat                                                               >  1:63120/1‑140 (MQ=255)                                                                           atatCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAAct                                                 <  1:425164/140‑1 (MQ=255)                                                                               cTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAActgctg                                             <  2:63120/140‑1 (MQ=255)                                                                                  ccgccAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAAt                                          >  1:270346/1‑140 (MQ=255)                                                                                  ccgccAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAAt                                          >  1:239515/1‑140 (MQ=255)                                                                                         gAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCtt                                   >  2:274156/1‑140 (MQ=255)                                                                                           aTTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGc                                 <  2:156759/140‑1 (MQ=255)                                                                                                tGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGCGAGaa                            <  1:223632/140‑1 (MQ=255)                                                                                                    ggcAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGCGAGAAatat                        >  2:102354/1‑140 (MQ=255)                                                                                                     gcAATAATACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGCGAGAAatata                       <  2:292479/140‑1 (MQ=255)                                                                                                       aataatACAAAATTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGCGAGAAATATATc                     <  2:270346/140‑1 (MQ=255)                                                                                                               aaaaTTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGCGAGAAATATATCCAttatta             <  2:283580/140‑1 (MQ=255)                                                                                                                 aaTTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGCGAGAAATATATCCAttatta             >  2:398752/1‑138 (MQ=255)                                                                                                                 aaTTCTTTGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGCGAGAAATATATCCAttatta             <  1:398752/138‑1 (MQ=255)                                                                                                                        tGCATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGCGAGAAATATATCCATTATTACATTTCATg    >  1:321586/1‑140 (MQ=255)                                                                                                                          cATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGATTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAACCTTCCTGGCGAGAAATATATCCATTATTACATTTCATGcc  <  2:106260/140‑1 (MQ=255)                                                                                                                          cATAAGAAATTTTTCGTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGCGAGAAATATATCCATTATTACATTTCATGcc  <  2:51555/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                             AAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGCGAGAAATATATCCATTATTACATTTCATGCC  >  NC_000913/238053‑238313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑