Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,005,664	C→A	S268* (TCG→TAG)	pldA →	outer membrane phospholipase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,005,664	0	C	A	100.0%	119.8 / NA	34	S268* (TCG→TAG)	pldA	outer membrane phospholipase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/22);  total (12/22)

CGGTGATGCGGTGCTCAGTGCGAAAGGACAGTACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAGAGACTAA  >  NC_000913/4005530‑4005794                                                                                                                                       |                                                                                                                                   cGGTGATGCGGTGCTCAGTGCGAAAGGACAGTACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCa                                                                                                                               <  2:132042/140‑1 (MQ=255)   gTGATGCGGTGCTCAGTGCGAAAGGACAGTACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATc                                                                                                                             <  2:382693/140‑1 (MQ=255)     gATGCGGTGCTCAGTGCGAAAGGACAGTACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGa                                                                                                                           >  1:105013/1‑140 (MQ=255)              cTCAGTGCGAAAGGACAGTACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACt                                                                                                                   <  1:206480/139‑1 (MQ=255)              cTCAGTGCGAAAGGACAGTACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACt                                                                                                                   >  2:206480/1‑139 (MQ=255)                  gTGCGAAAGGACAGTACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCTAATAGCTCATCGACTATAACTTCAAc                                                                                                              <  2:105013/140‑1 (MQ=255)                   tGCGAAAGGACAGTACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAAcc                                                                                                             >  1:478257/1‑140 (MQ=255)                   tGCGAAAGGACAGTACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAAcc                                                                                                             <  1:495764/140‑1 (MQ=255)                            aCAGTACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCgtg                                                                                                    <  1:410043/140‑1 (MQ=255)                             cAGTACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCgtgt                                                                                                   >  1:420099/1‑140 (MQ=255)                             cAGTACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCgtgt                                                                                                   <  2:284573/140‑1 (MQ=255)                              aGTACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTc                                                                                                  <  1:293013/140‑1 (MQ=255)                               gTACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCg                                                                                                 <  1:297349/140‑1 (MQ=255)                                         aaCACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGt                                                                                       <  1:483593/140‑1 (MQ=255)                                                 cTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTaa                                                                               <  2:325898/140‑1 (MQ=255)                                                     ggcggcGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGa                                                                           >  1:184094/1‑140 (MQ=255)                                                                   aGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGaa                                                             >  2:487916/1‑140 (MQ=255)                                                                   aGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGaa                                                             <  2:184094/140‑1 (MQ=255)                                                                   aGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGaa                                                             <  1:155969/140‑1 (MQ=255)                                                                   aGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGaa                                                             <  1:214437/140‑1 (MQ=255)                                                                                      aCCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCaaa                                          >  1:94603/1‑140 (MQ=255)                                                                                                tgCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATcc                                >  2:435094/1‑140 (MQ=255)                                                                                                 gcgcCTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCa                               <  2:478257/140‑1 (MQ=255)                                                                                                    ccTTTATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCa                            <  1:487916/140‑1 (MQ=255)                                                                                                      tttATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATc                          <  2:14098/140‑1 (MQ=255)                                                                                                      tttATACTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATc                          <  2:94603/140‑1 (MQ=255)                                                                                                            cTCAGGTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGAt                    <  2:207377/140‑1 (MQ=255)                                                                                                                 gTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTAccc               >  1:350789/1‑140 (MQ=255)                                                                                                                 gTTTACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTAccc               >  2:458487/1‑140 (MQ=255)                                                                                                                  tttACAGCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCct              <  1:410164/140‑1 (MQ=255)                                                                                                                        gCGGCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCaga        <  2:420099/140‑1 (MQ=255)                                                                                                                           gCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTAcc                <  1:73858/129‑1 (MQ=255)                                                                                                                           gCTATGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTAcc                >  2:73858/1‑129 (MQ=255)                                                                                                                              aTGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAGAGACTaa  <  2:350789/140‑1 (MQ=255)                                                                                                                              aTGGCGAATAGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAGAGACTaa  >  2:371359/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                   CGGTGATGCGGTGCTCAGTGCGAAAGGACAGTACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAGAGACTAA  >  NC_000913/4005530‑4005794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑