Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	358,111	G→T	L194I (CTC→ATC)	cynR ←	DNA‑binding transcriptional dual regulator CynR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	358,111	0	G	T	100.0%	66.2 / NA	20	L194I (CTC→ATC)	cynR	DNA‑binding transcriptional dual regulator CynR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/9);  total (11/9)

TGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGAGCAGGACCAGTTTTTCATCATGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGCGGATGATGTTGCGCCACGACTAACGCTAAACTTTCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACA  >  NC_000913/357977‑358244                                                                                                                                       |                                                                                                                                      tgtgGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGATCAGGa                                                                                                                                  >  2:10284/1‑140 (MQ=255) tgtgGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAATTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGATCAGGa                                                                                                                                  >  1:446648/1‑140 (MQ=255)  gtgGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGATCAGGAc                                                                                                                                 <  2:174339/140‑1 (MQ=255)            aaGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGATCAGGACCAGTTTTtca                                                                                                                       <  2:472267/140‑1 (MQ=255)                        tCAGCTCCAGAACCGCGCTAATTGAATTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGATCAGGACCAGTTTTTCATCATGCAAGCGa                                                                                                           <  2:446648/140‑1 (MQ=255)                                   aCCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGATCAGGACCAGTTTTTCATCATGCAAGCGACTCAACGCCAc                                                                                                <  1:60658/140‑1 (MQ=255)                                                      ccTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGATCAGGACCAGTTTTTCATCATGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGc                                                                             >  2:65943/1‑140 (MQ=255)                                                      ccTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGATCAGGACCAGTTTTTCATCATGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGc                                                                             >  1:456239/1‑140 (MQ=255)                                                      ccTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGATCAGGACCAGTTTTTCATCATGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGc                                                                             >  1:166812/1‑140 (MQ=255)                                                         cAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGATCAGGACCAGTTTTTCATCATGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGCGga                                                                          <  1:10284/140‑1 (MQ=255)                                                                 accTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGATCAGGACCAGTTTTTCATCATGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGCGGATGATGTTg                                                                  >  1:319589/1‑140 (MQ=255)                                                                          tGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGATCAGGACCAGTTTTTCATCATGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGCGGATGATGTTGCGCCACGAc                                                         >  1:66181/1‑140 (MQ=255)                                                                           gTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGATCAGGACCAGTTTTTCATCATGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGCGGATGATGTTGCGCCACGACt                                                        <  2:456239/140‑1 (MQ=255)                                                                             aGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGATCAGGACCAGTTTTTCATCATGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGCGGATGATGTTGCGCCACGACTaa                                                      >  1:369587/1‑140 (MQ=255)                                                                                                 gtGGCCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGATCAGGACCAGTTTTTCATCATGCAAGCGACACAACGCGACCTGTTCATGGACGGCCAGCGGATGATGTTGCGCCACGACTAACGCTAAACTTTCTGTCAGTa                                  >  2:132808/1‑140 (MQ=255)                                                                                                       aaTTTGCTCTCTGGTGGCAAATTCCGCGCTGATCAGGACCAGTTTTTCATCATGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGCGGATGATGTTGCGCCACGACTAACGCTAAACTTTCTGt                                       >  1:418295/1‑129 (MQ=255)                                                                                                       aaTTTGCTCTCTGGTGGCAAATTCCGCGCTGATCAGGACCAGTTTTTCATCATGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGCGGATGATGTTGCGCCACGACTAACGCTAAACTTTCTGt                                       <  2:418295/129‑1 (MQ=255)                                                                                                                  tggtggCAAATTCCGCGCTGATCAGGACCAGTTTTTCATCATGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGCGGATGATGTTGCGCCACGACTAACGCTAAACTTTCTGTCAGTAAAGGAATTGCCTCCAGc                 >  2:447200/1‑140 (MQ=255)                                                                                                                               cgcgCTGATCAGGACCAGTTTTTCATCATGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGCGGATGATGTTGCGCCACGACTAACGCTAAACTTTCTGTCAGTAAAGGAATTGCCTCCAGCTCCgg            >  2:239551/1‑132 (MQ=255)                                                                                                                               cgcgCTGATCAGGACCAGTTTTTCATCATGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGCGGATGATGTTGCGCCACGACTAACGCTAAACTTTCTGTCAGTAAAGGAATTGCCTCCAGCTCCgg            <  1:239551/132‑1 (MQ=255)                                                                                                                                 cgcTGATCAGGACCAGTTTTTCATCATGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGCGGATGATGTTGCGCCACGACTAACGCTAAACTTTCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGcaca  <  2:166812/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      TGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGAGCAGGACCAGTTTTTCATCATGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGCGGATGATGTTGCGCCACGACTAACGCTAAACTTTCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACA  >  NC_000913/357977‑358244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑