Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	99,275	G→T	L291L (CTG→CTT)	ftsW →	putative lipid II flippase FtsW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	99,275	0	G	T	100.0%	333.4 / NA	95	L291L (CTG→CTT)	ftsW	putative lipid II flippase FtsW
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (41/54);  total (41/54)

CGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAA  >  NC_000913/99138‑99408                                                                                                                                          |                                                                                                                                      cgTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTcc                                                                                                                                     >  1:1746145/1‑140 (MQ=255) cgTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCG‑TTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCg                                                                                                                                    <  2:91982/140‑1 (MQ=255)  gtgtTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCg                                                                                                                                    <  2:1096087/140‑1 (MQ=255)  gtgtTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCg                                                                                                                                    >  2:1609025/1‑140 (MQ=255)   tgtTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCgg                                                                                                                                   >  2:536625/1‑140 (MQ=255)     ttACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAGCTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGaa                                                                                                                                 >  2:1091209/1‑140 (MQ=255)     ttACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGaa                                                                                                                                 >  1:542614/1‑140 (MQ=255)           cATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGcac                                                                                                                           <  1:119356/140‑1 (MQ=255)           cATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGcac                                                                                                                           >  1:939733/1‑140 (MQ=255)           cATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGcac                                                                                                                           <  1:742205/140‑1 (MQ=255)            aTTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGcaca                                                                                                                          <  1:1289508/140‑1 (MQ=255)            aTTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGcaca                                                                                                                          <  1:1494550/140‑1 (MQ=255)            aTTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGcaca                                                                                                                          <  2:1517505/140‑1 (MQ=255)            aTTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGcaca                                                                                                                          <  1:1752941/140‑1 (MQ=255)             ttCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGca                                                                                                                            <  1:379907/137‑1 (MQ=255)             ttCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGca                                                                                                                            >  2:379907/1‑137 (MQ=255)             ttCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCacac                                                                                                                         <  2:1294437/140‑1 (MQ=255)             ttCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCacac                                                                                                                         >  2:63307/1‑140 (MQ=255)               cTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTAGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTg                                                                                                                       >  2:439110/1‑140 (MQ=255)                  gAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACt                                                                                                                    >  1:755466/1‑140 (MQ=255)                        gTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCacac                                                                                                                         >  1:1381210/1‑129 (MQ=255)                        gTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCacac                                                                                                                         <  2:1381210/129‑1 (MQ=255)                        gTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTAttt                                                                                                              >  1:256316/1‑140 (MQ=255)                        gTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTAttt                                                                                                              >  1:223652/1‑140 (MQ=255)                        gTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTAttt                                                                                                              >  2:42628/1‑140 (MQ=255)                        gTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTAttt                                                                                                              <  2:542614/140‑1 (MQ=255)                         tGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTAtttt                                                                                                             >  2:1798470/1‑140 (MQ=255)                          gggAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTc                                                                                                            >  1:1074510/1‑140 (MQ=255)                          gggAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTc                                                                                                            <  2:652381/140‑1 (MQ=255)                                aTCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCAtt                                                                                                      >  2:576833/1‑140 (MQ=255)                                aTCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCAtt                                                                                                      >  2:75580/1‑140 (MQ=255)                                       tGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCg                                                                                               >  2:110558/1‑140 (MQ=255)                                       tGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCg                                                                                               <  2:367667/140‑1 (MQ=255)                                       tGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCg                                                                                               <  2:939733/140‑1 (MQ=255)                                        ggCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAGCTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCga                                                                                              <  1:1091209/140‑1 (MQ=255)                                         gCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCgaa                                                                                             <  1:75580/140‑1 (MQ=255)                                          cAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCgaag                                                                                            >  1:158563/1‑140 (MQ=255)                                            gCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCgaagaa                                                                                          >  1:1085940/1‑140 (MQ=255)                                            gCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCACACACTGACTTTATTTTCGCCATTATCGGCgaagaa                                                                                          >  1:1798869/1‑140 (MQ=255)                                            gCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGAGCACACTGACTTTATTTTCGCCATTATCGGCgaagaa                                                                                          <  1:1136180/140‑1 (MQ=255)                                             cGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAAc                                                                                         >  2:408926/1‑140 (MQ=255)                                             cGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAAc                                                                                         >  2:1275055/1‑140 (MQ=255)                                               gCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTg                                                                                       <  1:1133943/140‑1 (MQ=255)                                                 tATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTggg                                                                                     <  2:807531/140‑1 (MQ=255)                                                 tATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTggg                                                                                     <  2:1746145/140‑1 (MQ=255)                                                   tCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGt                                                                                   >  1:95109/1‑140 (MQ=255)                                                       ttAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGt                                                                               <  2:95109/140‑1 (MQ=255)                                                       ttAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGt                                                                               <  1:63307/140‑1 (MQ=255)                                                       ttAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGt                                                                               <  1:42628/140‑1 (MQ=255)                                                       ttAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGt                                                                               <  2:256316/140‑1 (MQ=255)                                                         aaCGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCg                                                                             >  1:1821530/1‑140 (MQ=255)                                                          aCGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCgg                                                                            >  1:111285/1‑140 (MQ=255)                                                            gCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCTCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGgtg                                                                          <  1:444758/140‑1 (MQ=255)                                                             cAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGgtgt                                                                         <  2:111285/140‑1 (MQ=255)                                                               aTCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTg                                                                                       >  2:1770768/1‑124 (MQ=255)                                                               aTCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTg                                                                                       <  1:1770768/124‑1 (MQ=255)                                                               aTCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGgtgt                                                                         <  2:1702871/138‑1 (MQ=255)                                                               aTCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGgtgt                                                                         >  1:1702871/1‑138 (MQ=255)                                                               aTCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTgtgg                                                                       >  2:155117/1‑140 (MQ=255)                                                                tCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGt                                                                               <  1:1811455/131‑1 (MQ=255)                                                                tCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGt                                                                               >  2:1811455/1‑131 (MQ=255)                                                                 cGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTgtggtg                                                                     >  1:719955/1‑140 (MQ=255)                                                                      aTGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGc                                                                <  1:536625/140‑1 (MQ=255)                                                                      aTGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGc                                                                <  1:110558/140‑1 (MQ=255)                                                                      aTGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGc                                                                <  2:1408532/140‑1 (MQ=255)                                                                      aTGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCACACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGc                                                                <  2:1798869/140‑1 (MQ=255)                                                                         gCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACt                                                             <  2:760618/140‑1 (MQ=255)                                                                         gCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACt                                                             <  1:1275055/140‑1 (MQ=255)                                                                           gTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACttt                                                           <  2:1085940/140‑1 (MQ=255)                                                                             ttGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTa                                                         <  1:408926/140‑1 (MQ=255)                                                                              tGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCATACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTaa                                                        >  1:443397/1‑140 (MQ=255)                                                                              tGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACttt                                                           <  1:1509688/137‑1 (MQ=255)                                                                              tGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACttt                                                           >  2:1509688/1‑137 (MQ=255)                                                                              tGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTaa                                                        <  1:378537/140‑1 (MQ=255)                                                                              tGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTaa                                                        <  2:1697048/140‑1 (MQ=255)                                                                                gTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATg                                                      >  1:164901/1‑140 (MQ=255)                                                                                   gcggcgAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTa                                                   <  1:837122/140‑1 (MQ=255)                                                                                                 gggCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCttt                                     <  1:155117/140‑1 (MQ=255)                                                                                                    cAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTcg                                  >  1:640519/1‑140 (MQ=255)                                                                                                                 aCTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGAtt                     <  1:439110/140‑1 (MQ=255)                                                                                                                 aCTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGAtt                     <  2:108094/140‑1 (MQ=255)                                                                                                                  cTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTcgcg                                <  2:1189935/128‑1 (MQ=255)                                                                                                                  cTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTcgcg                                >  1:1189935/1‑128 (MQ=255)                                                                                                                  cTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTg                    >  1:642340/1‑140 (MQ=255)                                                                                                                       tACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGAt                      >  1:1266069/1‑133 (MQ=255)                                                                                                                       tACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGAt                      <  2:1266069/133‑1 (MQ=255)                                                                                                                        aCAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGAtt                     >  1:1675252/1‑133 (MQ=255)                                                                                                                        aCAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGAtt                     <  2:1675252/133‑1 (MQ=255)                                                                                                                        aCAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTa              <  1:1798470/140‑1 (MQ=255)                                                                                                                          aaaaaCTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTaaa            <  2:642340/140‑1 (MQ=255)                                                                                                                          aaaaaCTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTaaa            <  1:1609025/140‑1 (MQ=255)                                                                                                                          aaaaaCTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTaaa            <  2:1342084/140‑1 (MQ=255)                                                                                                                               cTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCAtt       <  2:719955/140‑1 (MQ=255)                                                                                                                               cTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCAtt       >  2:1416049/1‑140 (MQ=255)                                                                                                                                 ggAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAg     <  2:615761/140‑1 (MQ=255)                                                                                                                                    gTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGaaa  >  1:1146950/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      CGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAA  >  NC_000913/99138‑99408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑