Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,825,636	C→A	intergenic (‑11/+319)	spy ← / ← astE	ATP‑independent periplasmic chaperone/succinylglutamate desuccinylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,825,636	0	C	A	100.0%	300.6 / NA	86	intergenic (‑11/+319)	spy/astE	ATP‑independent periplasmic chaperone/succinylglutamate desuccinylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (39/47);  total (39/47)

CCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTA  >  NC_000913/1825501‑1825771                                                                                                                                            |                                                                                                                                        ccTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATAtcattcc                                                                                                                                     >  1:1291266/1‑140 (MQ=255)  cTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATAtcattcct                                                                                                                                    <  2:771121/140‑1 (MQ=255)    ttGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCttt                                                                                                                                  <  1:562906/140‑1 (MQ=255)    ttGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCttt                                                                                                                                  <  1:745543/140‑1 (MQ=255)       tggtgCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAg                                                                                                                               <  1:1133247/140‑1 (MQ=255)       tggtgCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAg                                                                                                                               <  2:1330488/140‑1 (MQ=255)        ggtgCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGt                                                                                                                              <  1:278022/140‑1 (MQ=255)           gCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGttat                                                                                                                           <  2:739101/140‑1 (MQ=255)            catcatCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGttatt                                                                                                                          >  2:1227382/1‑140 (MQ=255)                atcGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTtatttatt                                                                                                                      >  1:820501/1‑140 (MQ=255)                atcGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTtatttatt                                                                                                                      <  1:1146669/140‑1 (MQ=255)                 tcGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATttatta                                                                                                                     <  1:83651/140‑1 (MQ=255)                 tcGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATttatta                                                                                                                     >  1:783553/1‑140 (MQ=255)                    gCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACTAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACgg                                                                                                                  <  2:148648/140‑1 (MQ=255)                    gCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACgg                                                                                                                  <  1:1308382/140‑1 (MQ=255)                    gCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACgg                                                                                                                  <  2:41723/140‑1 (MQ=255)                    gCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACgg                                                                                                                  <  1:1226241/140‑1 (MQ=255)                    gCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACgg                                                                                                                  <  2:1484590/140‑1 (MQ=255)                      ttCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCt                                                                                                                <  1:1227382/140‑1 (MQ=255)                      ttCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCt                                                                                                                <  1:156568/140‑1 (MQ=255)                      ttCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCt                                                                                                                <  1:538206/140‑1 (MQ=255)                      ttCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCt                                                                                                                <  1:1672438/140‑1 (MQ=255)                        cgcgTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTCTATATATCATTCCTTTCAGTTATTTATTACGGCt                                                                                                                <  1:1610448/140‑1 (MQ=255)                        cgcgTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCttt                                                                                                              >  2:142163/1‑140 (MQ=255)                        cgcgTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCttt                                                                                                              >  1:1008983/1‑140 (MQ=255)                          cgTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCt                                                                                                            >  1:718703/1‑140 (MQ=255)                           gTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCtt                                                                                                           <  2:281869/140‑1 (MQ=255)                           gTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCtt                                                                                                           <  2:10616/140‑1 (MQ=255)                               gCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGt                                                                                                       >  2:271796/1‑140 (MQ=255)                               gCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGt                                                                                                       >  1:393384/1‑140 (MQ=255)                                     gctgcGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTg                                                                                                 <  1:144276/140‑1 (MQ=255)                                         cGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTACGCGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGcc                                                                                               >  1:1360136/1‑140 (MQ=255)                                         cGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTaa                                                                                                         <  2:1592175/128‑1 (MQ=255)                                         cGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTaa                                                                                                         >  1:1592175/1‑128 (MQ=255)                                         cGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCgt                                                                                             >  2:1443778/1‑140 (MQ=255)                                              gtggtgTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGa                                                                                        >  1:88142/1‑140 (MQ=255)                                              gtggtgTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGa                                                                                        <  2:1008983/140‑1 (MQ=255)                                              gtggtgTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGa                                                                                        <  1:1802066/140‑1 (MQ=255)                                                  tgtCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACgag                                                                                    <  2:1243452/140‑1 (MQ=255)                                                   gtCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACgaga                                                                                   >  2:1624596/1‑140 (MQ=255)                                                      tGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTa                                                                                >  2:742097/1‑140 (MQ=255)                                                      tGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTa                                                                                <  2:88142/140‑1 (MQ=255)                                                        cGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAAc                                                                              >  1:395813/1‑140 (MQ=255)                                                           cATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACttt                                                                           >  1:736119/1‑140 (MQ=255)                                                              gggCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGTGTGCCGTGTTGACGAGATTAACTTTACt                                                                        <  1:674184/140‑1 (MQ=255)                                                              gggCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGTGTGCCGTGTTGACGAGATTAACTTTACt                                                                        >  2:674184/1‑140 (MQ=255)                                                              gggCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACt                                                                        <  1:742097/140‑1 (MQ=255)                                                                gCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACgag                                                                                    <  1:987489/126‑1 (MQ=255)                                                                gCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACgag                                                                                    >  2:987489/1‑126 (MQ=255)                                                                     gTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTa                                                                 >  2:1598928/1‑140 (MQ=255)                                                                       tAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGc                                                               <  2:395813/140‑1 (MQ=255)                                                                          ccGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACttt                                                                           >  2:1677049/1‑125 (MQ=255)                                                                          ccGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACttt                                                                           <  1:1677049/125‑1 (MQ=255)                                                                          ccGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTc                                                            >  2:1698896/1‑140 (MQ=255)                                                                          ccGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTc                                                            >  1:1569156/1‑140 (MQ=255)                                                                          ccGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTc                                                            >  1:1415093/1‑140 (MQ=255)                                                                          ccGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑CATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTc                                                            >  1:586476/1‑140 (MQ=255)                                                                          ccGCGCCAAGAGCCAGGGTAAAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGATATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTc                                                            >  1:928812/1‑140 (MQ=255)                                                                               ccAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTa                                                       >  2:893904/1‑140 (MQ=255)                                                                                 aaGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGATATTAACTTTACTGACTTTAGCGTCAATTAAt                                                     <  1:144520/140‑1 (MQ=255)                                                                                 aaGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAAt                                                     <  2:736119/140‑1 (MQ=255)                                                                                       cAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGGTTAACTTTACTGACTTTAGCGTCAATTAATCAGAGc                                               <  1:703690/140‑1 (MQ=255)                                                                                       cAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCagggc                                               <  2:718703/140‑4 (MQ=255)                                                                                       cAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGc                                               <  1:979161/140‑1 (MQ=255)                                                                                       cAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGc                                               <  1:1598928/140‑1 (MQ=255)                                                                                       cAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGc                                               <  2:1415093/140‑1 (MQ=255)                                                                                        aGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCa                                              <  1:385426/140‑1 (MQ=255)                                                                                        aGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCa                                              >  1:1654404/1‑140 (MQ=255)                                                                                         gggTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCaa                                             <  2:783553/140‑1 (MQ=255)                                                                                          ggTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAAc                                            >  2:626678/1‑140 (MQ=255)                                                                                               agGCAACAAATAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTaa                                       <  2:928812/140‑1 (MQ=255)                                                                                               agGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTaa                                       >  1:1062375/1‑140 (MQ=255)                                                                                               agGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTaa                                       >  1:167110/1‑140 (MQ=255)                                                                                               agGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTaa                                       >  2:1688624/1‑140 (MQ=255)                                                                                               agGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTaa                                       <  2:1569156/140‑1 (MQ=255)                                                                                               agGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTaa                                       <  1:626678/140‑1 (MQ=255)                                                                                                    acaaacaGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACaa                                  >  1:1088946/1‑140 (MQ=255)                                                                                                     caaacaGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAAt                                 >  1:1086164/1‑140 (MQ=255)                                                                                                       aacaGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGa                               <  1:682619/140‑1 (MQ=255)                                                                                                       aacaGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGa                               <  1:1688624/140‑1 (MQ=255)                                                                                                           gTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAgt                           >  1:33192/1‑140 (MQ=255)                                                                                                              cAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTa                        <  2:64515/140‑1 (MQ=255)                                                                                                              cAGTTAATTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTa                        >  2:1607646/1‑140 (MQ=255)                                                                                                                    aTTTA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAac                  >  1:1248989/1‑140 (MQ=255)                                                                                                                      ttA‑‑CGCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAacac                >  1:302695/1‑140 (MQ=255)                                                                                                                            gCATATTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGAATAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACtttt            <  2:687800/140‑1 (MQ=255)                                                                                                                               tatTC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTgcg         <  2:1360136/140‑1 (MQ=255)                                                                                                                                 ttC‑‑TATATCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGcc       >  1:1770675/1‑140 (MQ=255)                                                                                                                                        tatCATTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTa  >  1:64705/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                        CCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTTAATTTA‑‑CGCATATTC‑‑TATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTA  >  NC_000913/1825501‑1825771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑