Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,906,534	G→T	S275I (AGC→ATC)	ygcG →	protein YgcG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,906,534	0	G	T	100.0%	155.7 / NA	45	S275I (AGC→ATC)	ygcG	protein YgcG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (6/39);  total (6/39)

TCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGA  >  NC_000913/2906404‑2906670                                                                                                                                   |                                                                                                                                         tCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCggggg                                                                                                                                 <  1:729333/140‑1 (MQ=255) tCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCggggg                                                                                                                                 >  1:670814/1‑140 (MQ=255)   cttcttTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGGTTTATCGGCggg                                                                                                                                   >  2:315942/1‑136 (MQ=255)   cttcttTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCggggggg                                                                                                                               >  1:425213/1‑138 (MQ=255)   cttcttTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCggg                                                                                                                                   <  1:1297556/136‑1 (MQ=255)   cttcttTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCggg                                                                                                                                   >  2:1297556/1‑136 (MQ=255)   cttcttTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCggg                                                                                                                                   <  1:315942/136‑1 (MQ=255)   cttcttTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCg                                                                                                                               >  1:13283/1‑140 (MQ=255)    ttcttTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCgg                                                                                                                              <  2:425213/140‑1 (MQ=255)    ttcttTTTTACTTTCACCAAATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCgg                                                                                                                              <  1:1065412/140‑1 (MQ=255)      cttTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCggggggggcg                                                                                                                            >  2:216430/1‑138 (MQ=255)        tttttACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCgg                                                                                                                              <  2:938127/136‑1 (MQ=255)        tttttACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCgg                                                                                                                              >  1:938127/1‑136 (MQ=255)                        atTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGgcggcg                                                                                                          <  2:10150/140‑1 (MQ=255)                                 cTTTACCTGTTTATGTGTACTTGAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGCCAACAGCGGTTCCGCGGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGTGCGGTTCTTCTGGAGGTGGCGGTGCATCCggac                                                                                                 <  2:1710459/140‑3 (MQ=255)                                 cTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGcc                                                                                                 <  1:671729/140‑1 (MQ=255)                                  tttACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCg                                                                                                <  1:400338/140‑1 (MQ=255)                                     aCCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTg                                                                                             <  1:387193/140‑1 (MQ=255)                                       cTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGt                                                                                           <  2:315270/140‑1 (MQ=255)                                         gTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTaa                                                                                         <  1:1197883/140‑1 (MQ=255)                                         gTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTaa                                                                                         <  2:13283/140‑1 (MQ=255)                                           ttATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACt                                                                                       <  1:241399/140‑1 (MQ=255)                                            tATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTc                                                                                      <  1:975623/140‑1 (MQ=255)                                             aTGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCa                                                                                     <  2:690279/140‑1 (MQ=255)                                                           aaaGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGc                                                                       <  1:307997/140‑1 (MQ=255)                                                           aaaGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGc                                                                       <  1:1121295/140‑1 (MQ=255)                                                           aaaGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGc                                                                       <  2:330116/140‑1 (MQ=255)                                                           aaaGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGc                                                                       <  1:91761/140‑1 (MQ=255)                                                            aaGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGcc                                                                      <  1:1527917/140‑1 (MQ=255)                                                                          acaGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTgg                                                        <  1:1614324/138‑1 (MQ=255)                                                                           aaGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGc                                                       <  1:423885/140‑1 (MQ=255)                                                                                   aTTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTaa                                               <  2:670814/140‑1 (MQ=255)                                                                                    ttCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTaaa                                              <  1:1242883/140‑1 (MQ=255)                                                                                     tCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAAt                                             <  2:315540/140‑1 (MQ=255)                                                                                          caacaGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGAt                                        <  1:1594795/140‑1 (MQ=255)                                                                                           aacaGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATa                                       <  1:1428756/140‑1 (MQ=255)                                                                                               gCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAagt                                   <  1:1308422/140‑1 (MQ=255)                                                                                                cGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAagtc                                  <  2:596739/140‑1 (MQ=255)                                                                                                     ccGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGTGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCt                             <  2:1169412/140‑1 (MQ=255)                                                                                                     ccGCTGGAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCt                             <  2:427811/140‑1 (MQ=255)                                                                                                           gAGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGcc                       <  2:739553/140‑1 (MQ=255)                                                                                                            aGGCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCt                      <  2:680173/140‑1 (MQ=255)                                                                                                              gCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTgg                    <  2:1690736/140‑1 (MQ=255)                                                                                                              gCTCAGATAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTgg                    <  1:216430/140‑1 (MQ=255)                                                                                                                    aTAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTg              <  1:573065/140‑1 (MQ=255)                                                                                                                    aTAGCGGCGGATTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTg              <  2:1149922/140‑1 (MQ=255)                                                                                                                              aTTTATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTAc    <  1:54445/140‑1 (MQ=255)                                                                                                                                ttATCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGa  <  1:147702/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         TCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGA  >  NC_000913/2906404‑2906670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑