BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,242,322	G→T	Y331* (TAC→TAA)	uxaA ←	D‑altronate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,242,322	0	G	T	99.0%	349.5 / ‑3.3	102	Y331* (TAC→TAA)	uxaA	D‑altronate dehydratase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (48/53); total (48/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 105 total aligned reads displayed.

ACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATC  >  NC_000913/3242194‑3242457

aCGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGaa                                                                                                                              <  1:184313/140‑1 (MQ=255)

 cGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAg                                                                                                                             <  1:1600899/140‑1 (MQ=255)

 cGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAg                                                                                                                             <  2:42404/140‑1 (MQ=255)

   tCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTc                                                                                                                           <  1:258977/140‑1 (MQ=255)

      aCCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGtt                                                                                                                        <  2:632268/140‑1 (MQ=255)

        cACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGa                                                                                                                      <  2:291013/140‑1 (MQ=255)

        cACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGa                                                                                                                      <  2:1166745/140‑1 (MQ=255)

           gacgCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCa                                                                                                                   <  2:680830/140‑1 (MQ=255)

             cgCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATg                                                                                                                 <  1:1148576/140‑1 (MQ=255)

             cgCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATg                                                                                                                 >  1:1502209/1‑140 (MQ=255)

                  gAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGAc                                                                                                            >  2:1297068/1‑140 (MQ=255)

                  gAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGAc                                                                                                            >  1:1520323/1‑140 (MQ=255)

                  gAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGAc                                                                                                            <  1:1297068/140‑1 (MQ=255)

                  gAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGAc                                                                                                            <  2:333791/140‑1 (MQ=255)

                       cGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGtt                                                                                                       >  2:1267895/1‑140 (MQ=255)

                         cTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGtttt                                                                                                     >  1:63695/1‑140 (MQ=255)

                           ttCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTc                                                                                                   <  1:491156/140‑1 (MQ=255)

                            tCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCa                                                                                                  >  1:1446896/1‑140 (MQ=255)

                            tCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCa                                                                                                  >  2:699258/1‑140 (MQ=255)

                             cTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCaa                                                                                                 <  2:637807/140‑1 (MQ=255)

                                ggTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACg                                                                                              <  2:1001174/140‑1 (MQ=255)

                                  tACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGAc                                                                                                            >  1:878460/1‑124 (MQ=255)

                                      gCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCtt                                                                                        >  1:451514/1‑140 (MQ=255)

                                         aaGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTtcgt                                                                                     <  1:1718441/140‑1 (MQ=255)

                                          aGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTtcgtc                                                                                    >  2:1050146/1‑140 (MQ=255)

                                          aGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTtcgtc                                                                                    >  1:1023344/1‑140 (MQ=255)

                                          aGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTtcgtc                                                                                    >  2:1440159/1‑140 (MQ=255)

                                           gTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTtcgtcg                                                                                   >  1:349782/1‑140 (MQ=255)

                                            tGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTcgc                                                                                  >  1:1763133/1‑140 (MQ=255)

                                               tttGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGc                                                                               <  2:1291213/140‑1 (MQ=255)

                                               tttGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATAGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGc                                                                               >  1:1521241/1‑140 (MQ=255)

                                                 tGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCaa                                                                             <  2:1520323/140‑1 (MQ=255)

                                                 tGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCaa                                                                             <  2:1763133/140‑1 (MQ=255)

                                                 tGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCaa                                                                             <  2:63695/140‑1 (MQ=255)

                                                     ttCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGt                                                                         <  2:349782/140‑1 (MQ=255)

                                                      tCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTc                                                                        >  1:1506965/1‑140 (MQ=255)

                                                          gCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATc                                                                    <  2:708548/140‑1 (MQ=255)

                                                             tggtgATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGc                                                                 >  2:1550428/1‑140 (MQ=255)

                                                             tggtgATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGc                                                                 >  1:1143737/1‑140 (MQ=255)

                                                             tggtgATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGc                                                                 >  2:560667/1‑140 (MQ=255)

                                                             tggtgATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGc                                                                 >  1:71906/1‑140 (MQ=255)

                                                             tggtgATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGc                                                                 >  1:697335/1‑140 (MQ=255)

                                                                    aCCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGct                                                          <  2:71906/140‑1 (MQ=255)

                                                                        cccgcTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAg                                                                  <  2:287795/128‑1 (MQ=255)

                                                                        cccgcTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAg                                                                  >  1:287795/1‑128 (MQ=255)

                                                                         ccgcTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTgcg                                                     <  1:560410/140‑1 (MQ=255)

                                                                          cgcTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTgcgc                                                    >  1:1090977/1‑140 (MQ=255)

                                                                          cgcTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTgcgc                                                    >  1:288404/1‑140 (MQ=255)

                                                                             tttGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCaa                                                 <  2:702517/140‑1 (MQ=255)

                                                                             tttGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCaa                                                 <  2:1023344/140‑1 (MQ=255)

                                                                                 ttCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACAt                                             <  2:1143737/140‑1 (MQ=255)

                                                                                       ggCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCgg                                       >  1:442198/1‑140 (MQ=255)

                                                                                         cGATAGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCa                                     <  2:1521241/140‑1 (MQ=255)

                                                                                           aTGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTgcg                                                     >  1:1100753/1‑122 (MQ=255)

                                                                                           aTGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTgcg                                                     <  2:1100753/122‑1 (MQ=255)

                                                                                           aTGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACt                                   >  1:67146/1‑140 (MQ=255)

                                                                                           aTGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACt                                   >  2:1228145/1‑140 (MQ=255)

                                                                                           aTGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACt                                   >  2:1219578/1‑140 (MQ=255)

                                                                                           aTGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACt                                   <  2:1012017/140‑1 (MQ=255)

                                                                                           aTGGGTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACt                                   <  1:775672/140‑1 (MQ=255)

                                                                                              ggTTTTCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCg                                <  1:166937/140‑1 (MQ=255)

                                                                                                ttttCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGt                              <  2:1090977/140‑1 (MQ=255)

                                                                                                ttttCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGt                              <  2:1055081/140‑1 (MQ=255)

                                                                                                ttttCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGt                              <  1:1050146/140‑1 (MQ=255)

                                                                                                ttttCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGt                              <  2:527724/140‑1 (MQ=255)

                                                                                                ttttCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGt                              <  1:1267895/140‑1 (MQ=255)

                                                                                                ttttCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGt                              <  2:1446896/140‑1 (MQ=255)

                                                                                                ttttCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGt                              <  1:1440159/140‑1 (MQ=255)

                                                                                                 tttCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTc                             <  1:168299/140‑1 (MQ=255)

                                                                                                   tCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAg                           <  1:1752098/140‑1 (MQ=255)

                                                                                                   tCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAg                           <  2:583959/140‑1 (MQ=255)

                                                                                                   tCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAg                           <  2:442198/140‑1 (MQ=255)

                                                                                                   tCATAGATCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAg                           <  1:1228145/140‑1 (MQ=255)

                                                                                                   tCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAg                           <  1:1755906/140‑1 (MQ=255)

                                                                                                         aTCGGTTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGgt                     >  2:705633/1‑140 (MQ=255)

                                                                                                         aTCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGgt                     >  2:152981/1‑140 (MQ=255)

                                                                                                         aTCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGgt                     >  1:954793/1‑140 (MQ=255)

                                                                                                         aTCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGgt                     >  2:1024261/1‑140 (MQ=255)

                                                                                                          tCGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGgta                    >  1:618209/1‑140 (MQ=255)

                                                                                                           cGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGt                              <  2:651909/129‑1 (MQ=255)

                                                                                                           cGGCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGt                              >  1:651909/1‑129 (MQ=255)

                                                                                                             gCTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGgtagta                 >  2:658568/1‑140 (MQ=255)

                                                                                                              cTGATCATGGGCAATAATTTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTTGGTCAGTACGGTATTAc                >  2:1823273/1‑140 (MQ=255)

                                                                                                              cTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTAc                >  2:1484914/1‑140 (MQ=255)

                                                                                                              cTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTAc                >  1:103743/1‑140 (MQ=255)

                                                                                                              cTGATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTAc                >  1:995880/1‑140 (MQ=255)

                                                                                                                gATCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTAccg              <  2:697335/140‑1 (MQ=255)

                                                                                                                 aTCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTAccgc             <  1:658568/140‑1 (MQ=255)

                                                                                                                 aTCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTAccgc             >  2:461254/1‑140 (MQ=255)

                                                                                                                  tCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTAccgcc            >  2:1530878/1‑140 (MQ=255)

                                                                                                                  tCATGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTAccacc            <  1:1001786/140‑4 (MQ=255)

                                                                                                                    aTGGGCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGt          <  2:67146/140‑1 (MQ=255)

                                                                                                                        gCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGgta                    >  1:1074319/1‑126 (MQ=255)

                                                                                                                        gCAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGgta                    <  2:1074319/126‑1 (MQ=255)

                                                                                                                         cAATAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCa     <  1:461254/140‑1 (MQ=255)

                                                                                                                           aTAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAAt   >  1:1106376/1‑140 (MQ=255)

                                                                                                                           aTAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAAt   >  1:20754/1‑140 (MQ=255)

                                                                                                                            tAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATc  >  1:190808/1‑140 (MQ=255)

                                                                                                                            tAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATc  >  1:1033867/1‑140 (MQ=255)

                                                                                                                            tAAATTACTGTTTGAAGTCGTTGACCATGGTGACCAGATTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATc  >  1:1801696/1‑140 (MQ=255)

ACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCTTCGTCGCGGCAATGGTCCATCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATC  >  NC_000913/3242194‑3242457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`