Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,924,708	G→T	P309Q (CCG→CAG)	mnmG ←	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,924,708	0	G	T	100.0%	288.1 / NA	83	P309Q (CCG→CAG)	mnmG	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (47/36);  total (47/36)

TTCAGGTCGCGAGGATCGAAGAAGTCATACTCAATGGCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACAT  >  NC_000913/3924573‑3924844                                                                                                                                        |                                                                                                                                         ttCAGGTCGCGAGGATCGAAGAAGTCATACTCAATGGCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATa                                                                                                                                      <  1:576823/140‑1 (MQ=255)        cgcgAGGATCGAAGAAGTCATACTCAATGGCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCa                                                                                                                               <  2:1583378/140‑1 (MQ=255)          cgAGGATCGAAGAAGTCATACTCAATGGCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGa                                                                                                                                        >  1:1785925/1‑129 (MQ=255)          cgAGGATCGAAGAAGTCATACTCAATGGCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCAtt                                                                                                                             >  2:1400122/1‑140 (MQ=255)           gAGGATCGAAGAAGTCATACTCAATGGCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTa                                                                                                                            <  2:516847/140‑1 (MQ=255)           gAGGATCGAAGAAGTCATACTCAATGGCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTa                                                                                                                            <  2:757534/140‑1 (MQ=255)                tCGAAGAAGTCATACTCAATGGCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGt                                                                                                                       <  1:512489/140‑1 (MQ=255)                         tCATACTCAATGGCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCtta                                                                                                              <  1:1546133/140‑2 (MQ=255)                           aTACTCAATGGCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCg                                                                                                            >  2:445966/1‑140 (MQ=255)                             aCTCAATGGCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGt                                                                                                          >  2:1027325/1‑140 (MQ=255)                             aCTCAATGGCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGt                                                                                                          >  2:390307/1‑140 (MQ=255)                              atCAATGGCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGtt                                                                                                         <  2:1656729/139‑1 (MQ=255)                                 aaTGGCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTc                                                                                                              >  1:1418013/1‑132 (MQ=255)                                 aaTGGCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTc                                                                                                              <  2:1418013/132‑1 (MQ=255)                                  aTGGCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAg                                                                                                     >  2:47349/1‑140 (MQ=255)                                   tGGCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAgg                                                                                                    >  2:31422/1‑140 (MQ=255)                                   tGGCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAgg                                                                                                    >  1:1722104/1‑140 (MQ=255)                                   tGGCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAACTTCATTAGAGGTCAGTCCTTCCGGTTCAAgg                                                                                                    >  2:938503/1‑140 (MQ=255)                                    ggCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGa                                                                                                   <  1:721788/140‑1 (MQ=255)                                    ggCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGa                                                                                                   <  1:718646/140‑1 (MQ=255)                                      cATAACCCGGTCACATGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGCCGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAg                                                                                                 <  2:732620/140‑1 (MQ=255)                                         aaCCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATc                                                                                              >  2:597597/1‑140 (MQ=255)                                         aaCCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGAAACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGGTCAAGGAAGATc                                                                                              >  2:1160724/1‑140 (MQ=255)                                           cccGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTg                                                                                            >  1:1255695/1‑140 (MQ=255)                                             cGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGAt                                                                                          <  2:1722104/140‑1 (MQ=255)                                               gACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGc                                                                                        >  1:318982/1‑140 (MQ=255)                                               gACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGc                                                                                        >  1:820019/1‑140 (MQ=255)                                               gACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGc                                                                                        >  2:330739/1‑140 (MQ=255)                                               gACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGc                                                                                        >  1:1373488/1‑140 (MQ=255)                                                     cGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGAttt                                                                                  >  1:1243401/1‑140 (MQ=255)                                                              cgTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCg                                                                         <  1:520908/140‑1 (MQ=255)                                                                  ttCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAgc                                                                     >  2:779993/1‑140 (MQ=255)                                                                  ttCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAgc                                                                     >  1:689160/1‑140 (MQ=255)                                                                  ttCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAgc                                                                     >  2:178680/1‑140 (MQ=255)                                                                  ttCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAgc                                                                     >  2:128432/1‑140 (MQ=255)                                                                  ttCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAgc                                                                     >  1:1776412/1‑140 (MQ=255)                                                                     cATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCa                                                                  <  2:1531292/140‑1 (MQ=255)                                                                          ccTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACt                                                             <  1:47349/140‑1 (MQ=255)                                                                          ccTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACt                                                             <  1:390307/140‑1 (MQ=255)                                                                           cTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACtt                                                            <  1:779993/140‑1 (MQ=255)                                                                             gCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTg                                                          >  2:572464/1‑140 (MQ=255)                                                                             gCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTg                                                          <  2:820019/140‑1 (MQ=255)                                                                               aTAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTc                                                        >  1:1221152/1‑140 (MQ=255)                                                                                   agCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTtcg                                                    >  2:341860/1‑140 (MQ=255)                                                                                   agCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTtcg                                                    >  2:957042/1‑140 (MQ=255)                                                                                   agCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTtcg                                                    >  1:1581488/1‑140 (MQ=255)                                                                                   agCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTtcg                                                    >  1:723231/1‑140 (MQ=255)                                                                                     cGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTtcgat                                                  >  1:369604/1‑140 (MQ=255)                                                                                       gACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTtcgatcg                                                <  2:1612020/140‑1 (MQ=255)                                                                                       gACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTtcgatcg                                                <  2:1221152/140‑1 (MQ=255)                                                                                        aCGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTtcgatcga                                               >  1:626096/1‑140 (MQ=255)                                                                                        aCGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTtcgatcga                                               >  2:704141/1‑140 (MQ=255)                                                                                        aCGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTtcgatcga                                               >  1:432742/1‑140 (MQ=255)                                                                                        aCGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTtcgatcga                                               >  1:717690/1‑140 (MQ=255)                                                                                         cGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGAc                                              <  2:432742/140‑1 (MQ=255)                                                                                          gATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACg                                             <  1:341860/140‑1 (MQ=255)                                                                                             ttGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGc                                          >  1:1737643/1‑140 (MQ=255)                                                                                               gCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAg                                        <  1:128432/140‑1 (MQ=255)                                                                                                    tGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCg                                   >  1:952504/1‑140 (MQ=255)                                                                                                      cacaTCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTg                                 >  1:277695/1‑140 (MQ=255)                                                                                                      cacaTCGAACGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTg                                 >  1:723262/1‑140 (MQ=255)                                                                                                             aaCGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGac                          >  1:691290/1‑140 (MQ=255)                                                                                                             aaCGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGac                          >  2:1764697/1‑140 (MQ=255)                                                                                                             aaCGGCAGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGac                          >  1:1074955/1‑140 (MQ=255)                                                                                                                   aGGCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGTGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTc                    >  1:302315/1‑140 (MQ=255)                                                                                                                     gCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGa                  >  2:896728/1‑140 (MQ=255)                                                                                                                     gCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGa                  >  2:915677/1‑140 (MQ=255)                                                                                                                     gCTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGAGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGa                  >  2:1371341/1‑140 (MQ=255)                                                                                                                      cTGGTGGAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGAt                 <  1:1094568/140‑1 (MQ=255)                                                                                                                       tggtggAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATc                >  2:11241/1‑140 (MQ=255)                                                                                                                        ggtggAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCa               <  1:572464/140‑1 (MQ=255)                                                                                                                         gtggAGATACCGTTCTGATAAACTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCAc              <  1:938503/140‑1 (MQ=255)                                                                                                                          tggAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCAcc             <  2:318982/140‑1 (MQ=255)                                                                                                                          tggAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCAcc             <  1:445966/140‑1 (MQ=255)                                                                                                                          tggAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCAcc             >  1:468886/1‑140 (MQ=255)                                                                                                                          tggAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCAcc             <  1:471701/140‑1 (MQ=255)                                                                                                                          tggAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCAcc             <  2:1581488/140‑1 (MQ=255)                                                                                                                           ggAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCAccc            <  1:1400122/140‑1 (MQ=255)                                                                                                                           ggAGATACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCAccc            <  1:457749/140‑1 (MQ=255)                                                                                                                               aTACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGc        >  1:193201/1‑140 (MQ=255)                                                                                                                               aTACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGAGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGc        <  1:1371341/140‑1 (MQ=255)                                                                                                                                tACCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCg       <  1:957042/140‑1 (MQ=255)                                                                                                                                 aCCGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGt      >  2:347938/1‑140 (MQ=255)                                                                                                                                   cGTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTAc    >  1:452494/1‑140 (MQ=255)                                                                                                                                    gTTCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACa   <  2:277695/140‑1 (MQ=255)                                                                                                                                     ttCTGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACAt  <  2:452494/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TTCAGGTCGCGAGGATCGAAGAAGTCATACTCAATGGCATAACCCGGACGCACGATCTTCGCGTTTTCCATCCCCTGCATAGAGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACAT  >  NC_000913/3924573‑3924844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑