BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,611,524	C→A	V43V (GTC→GTA)	osmY →	periplasmic chaperone OsmY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,611,524	0	C	A	100.0%	378.3 / NA	107	V43V (GTC→GTA)	osmY	periplasmic chaperone OsmY
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (44/63); total (44/63)

Only 100 of 109 total aligned reads displayed.

AATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTCGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAAGAGCACCGATATCTCTGTAAAAACCGATCAAAAAGTCGTGA  >  NC_000913/4611387‑4611657

aaTAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGa                                                                                                                                     <  2:521983/140‑1 (MQ=255)

aaTAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGa                                                                                                                                     <  1:480001/140‑1 (MQ=255)

 aTAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGAt                                                                                                                                    >  2:473806/1‑140 (MQ=255)

 aTAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGAt                                                                                                                                    >  1:151882/1‑140 (MQ=255)

 aTAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGAt                                                                                                                                    >  1:1486033/1‑140 (MQ=255)

  tAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATa                                                                                                                                   <  1:528261/140‑1 (MQ=255)

   aaaTCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAg                                                                                                                                  <  2:534695/140‑1 (MQ=255)

   aaaTCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAg                                                                                                                                  >  1:1325187/1‑140 (MQ=255)

    aaTCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGc                                                                                                                                 <  2:121417/140‑1 (MQ=255)

     aTCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGct                                                                                                                                <  2:883069/140‑1 (MQ=255)

      tCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGctc                                                                                                                               <  1:746957/140‑1 (MQ=255)

       cGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGctct                                                                                                                              >  2:699771/1‑140 (MQ=255)

       cGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGctct                                                                                                                              >  2:1404161/1‑140 (MQ=255)

         aTGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAg                                                                                                                                  >  2:794295/1‑134 (MQ=255)

         aTGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAg                                                                                                                                  <  1:794295/134‑1 (MQ=255)

         aTGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTAt                                                                                                                            >  1:1148492/1‑140 (MQ=255)

         aTGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTAt                                                                                                                            >  1:150181/1‑140 (MQ=255)

         aTGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTAt                                                                                                                            >  1:1127865/1‑140 (MQ=255)

         aTGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTAt                                                                                                                            >  1:179968/1‑140 (MQ=255)

          tGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATg                                                                                                                           >  1:371455/1‑140 (MQ=255)

              tATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATa                                                                                                                       >  2:446495/1‑140 (MQ=255)

                   cAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTc                                                                                                                  <  1:45626/140‑1 (MQ=255)

                      gACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGt                                                                                                               <  1:1003846/140‑1 (MQ=255)

                      gACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGt                                                                                                               <  2:11173/140‑1 (MQ=255)

                      gACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGt                                                                                                               <  1:1303753/140‑1 (MQ=255)

                      gACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGt                                                                                                               >  1:1118674/1‑140 (MQ=255)

                       aCTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCTAAAAGTAGATAGCTCTATGAATAAAGACGGTa                                                                                                              >  2:1374480/1‑140 (MQ=255)

                       aCTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTa                                                                                                              >  2:704913/1‑140 (MQ=255)

                        cTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTaa                                                                                                             >  2:477506/1‑140 (MQ=255)

                        cTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTaa                                                                                                             >  1:1240897/1‑140 (MQ=255)

                        cTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTaa                                                                                                             >  1:983330/1‑140 (MQ=255)

                        cTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTaa                                                                                                             >  1:972745/1‑140 (MQ=255)

                          gAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAAtt                                                                                                           >  1:1709090/1‑140 (MQ=255)

                          gAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAAtt                                                                                                           <  2:523696/140‑1 (MQ=255)

                          gAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAAtt                                                                                                           >  2:1295960/1‑140 (MQ=255)

                           aaGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTaa                                                                                                             <  2:1606922/137‑1 (MQ=255)

                           aaGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTaa                                                                                                             >  1:1606922/1‑137 (MQ=255)

                           aaGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAAttt                                                                                                          >  2:69837/1‑140 (MQ=255)

                           aaGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAAttt                                                                                                          >  2:800136/1‑140 (MQ=255)

                           aaGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAAttt                                                                                                          >  1:1011411/1‑140 (MQ=255)

                                ttCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATgg                                                                                                     >  2:1787857/1‑140 (MQ=255)

                                ttCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATgg                                                                                                     >  2:1341485/1‑140 (MQ=255)

                                 tCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGa                                                                                                    >  2:79479/1‑140 (MQ=255)

                                 tCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGa                                                                                                    <  2:438069/140‑1 (MQ=255)

                                   gAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATg                                                                                                  <  1:764200/140‑1 (MQ=255)

                                     aaaCTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGAc                                                                                                <  1:79479/140‑1 (MQ=255)

                                     aaaCTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGAc                                                                                                <  2:1180359/140‑1 (MQ=255)

                                           tgctgGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGcc                                                                                          <  1:446495/140‑1 (MQ=255)

                                             ctggctgTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCAt                                                                                        <  2:1724366/140‑1 (MQ=255)

                                               ggctgTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCa                                                                                      <  2:1699701/140‑1 (MQ=255)

                                               ggctgTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCa                                                                                      <  2:1127865/140‑1 (MQ=255)

                                                  tgTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACcg                                                                                   >  2:1754543/1‑140 (MQ=255)

                                                    tAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACcgcg                                                                                 <  2:1240897/140‑1 (MQ=255)

                                                    tAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACcgcg                                                                                 <  2:1486033/140‑1 (MQ=255)

                                                      aTGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGaa                                                                               >  2:953410/1‑140 (MQ=255)

                                                              ctctGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAgg                                                                       >  2:544528/1‑140 (MQ=255)

                                                              ctctGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGACAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGG‑TGACAGCGCCATCACCGCGAAAGTGAAggc                                                                      <  1:1404161/140‑1 (MQ=255)

                                                                 tGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAggcgg                                                                    <  2:151882/140‑1 (MQ=255)

                                                                 tGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAggcgg                                                                    <  1:1029098/140‑1 (MQ=255)

                                                                  gCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAggcggc                                                                   <  1:473806/140‑1 (MQ=255)

                                                                   ccGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGcc                                                                  <  1:1472595/140‑1 (MQ=255)

                                                                    cGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGccc                                                                 <  1:25768/140‑1 (MQ=255)

                                                                    cGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGccc                                                                 <  1:1809255/140‑1 (MQ=255)

                                                                       cgcgACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGGCCtgg                                                              <  2:1131388/140‑1 (MQ=255)

                                                                       cgcgACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCtgg                                                              <  2:371455/140‑1 (MQ=255)

                                                                       cgcgACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCtgg                                                              <  1:412424/140‑1 (MQ=255)

                                                                       cgcgACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCtgg                                                              <  1:450190/140‑1 (MQ=255)

                                                                       cgcgACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCtgg                                                              <  1:1635899/140‑1 (MQ=255)

                                                                        gcgACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCtggt                                                             <  1:704913/140‑1 (MQ=255)

                                                                                 tctGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGa                                                    <  2:1118674/140‑1 (MQ=255)

                                                                                  ctGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGac                                                   <  2:1776348/140‑1 (MQ=255)

                                                                                  ctGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGac                                                   <  1:69837/140‑1 (MQ=255)

                                                                                   tGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGaca                                                  <  2:1539311/140‑1 (MQ=255)

                                                                                   tGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGaca                                                  <  2:1709090/140‑1 (MQ=255)

                                                                                     ccTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGacaac                                                <  2:1148492/140‑1 (MQ=255)

                                                                                        aCGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATc                                             <  1:544528/140‑1 (MQ=255)

                                                                                          gcgGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCaa                                           >  2:1096833/1‑140 (MQ=255)

                                                                                           cgGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAag                                          <  1:1601445/140‑1 (MQ=255)

                                                                                            ggAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAaga                                         <  1:451038/140‑1 (MQ=255)

                                                                                            ggAAAACAACGCGCAGACTAACAAAGAAAGCGCGGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGGCCTGGTGGATCATGACAACATCAaga                                         <  1:216870/140‑1 (MQ=255)

                                                                                             gAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAagag                                        <  2:1559243/140‑1 (MQ=255)

                                                                                             gAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGC‑AAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAAGAGc                                       <  1:1058004/140‑1 (MQ=255)

                                                                                                       cgcAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAAGAGCACCGATAtc                              <  2:1759093/140‑1 (MQ=255)

                                                                                                       cgcAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAAGAGCACCGATAtc                              <  2:669670/140‑1 (MQ=255)

                                                                                                        gcAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAAGAGCTCCGATAtct                             <  2:1325187/140‑1 (MQ=255)

                                                                                                        gcAGACTACCAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAAGAGCACCGATAtct                             >  2:1350208/1‑140 (MQ=255)

                                                                                                                ccAATGAAAGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAAGAGCACCGATATCTCTGTaaaa                     <  2:972745/140‑1 (MQ=255)

                                                                                                                      aaaGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAAGAGCACCGATAtc                              >  2:1114776/1‑125 (MQ=255)

                                                                                                                      aaaGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAAGAGCACCGATAtc                              <  1:1114776/125‑1 (MQ=255)

                                                                                                                        aGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAAGAGCACCGATATCTCTGTaaaaa                    <  2:881649/133‑1 (MQ=255)

                                                                                                                        aGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAAGAGCACCGATATCTCTGTaaaaa                    >  1:881649/1‑133 (MQ=255)

                                                                                                                        aGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAAGAGCACCGATATCTCTGTAAAAACCGATCa             >  2:1299023/1‑140 (MQ=255)

                                                                                                                        aGCGCAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAAGAGCACCGATATCTCTGTAAAAACCGATCa             >  1:609778/1‑140 (MQ=255)

                                                                                                                          cgcAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAAGAGCACCGATATCTCTGTAAAAACCGATCaaa           >  2:521738/1‑140 (MQ=255)

                                                                                                                          cgcAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAAGAGCACCGATATCTCTGTAAAAACCGATCaaa           >  1:1079114/1‑140 (MQ=255)

                                                                                                                          cgcAGGGCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAAGAGCACCGATATCTCTGTAAAAACCGATCaaa           >  2:1397060/1‑140 (MQ=255)

                                                                                                                               ggCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAAGAGCACCGATATCTCTGTAAAAACCGATCAAAAAGTc      >  1:486133/1‑140 (MQ=255)

                                                                                                                                gCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAAGAGCACCGATATCTCTGTAAAAACCGATCAAAAAGTCg     <  1:1397060/140‑1 (MQ=255)

                                                                                                                                gCAAAAAGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTG‑AGGCGGCCCTGGTGGATCATGACAACATCAAGAGCACCGATATCTCTGTAAAAACCGATCAAAAAGTCGt    >  2:29861/1‑140 (MQ=255)

                                                                                                                                   aaaaGTAGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAAGAGCACCGATATCTCTGTAAAAACCGATCAAAAAGTCGTGa  <  1:1501627/140‑1 (MQ=255)

AATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCTGTAATGTTGACCTCTGCCGTCGCGACCGGCTCTGCCTACGCGGAAAACAACGCGCAGACTACCAATGAAAGCGCAGGGCAAAAAGTCGATAGCTCTATGAATAAAGTCGGTAATTTCATGGATGACAGCGCCATCACCGCGAAAGTGAAGGCGGCCCTGGTGGATCATGACAACATCAAGAGCACCGATATCTCTGTAAAAACCGATCAAAAAGTCGTGA  >  NC_000913/4611387‑4611657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`