| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,218,525 | C→A | A11A (GCG→GCT) | yehY ← | glycine betaine ABC transporter membrane subunit YehY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,218,525 | 0 | C | A | 98.1% | 180.2 / ‑2.8 | 53 | A11A (GCG→GCT) | yehY | glycine betaine ABC transporter membrane subunit YehY |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (29/23); total (30/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
CAACCAACGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAA > NC_000913/2218388‑2218656 | caaccaacGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAgcgc < 1:1930057/140‑1 (MQ=255)caaccaacGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAgagc < 2:1486134/140‑1 (MQ=255)caaccaacGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAgagc < 2:1497868/140‑1 (MQ=255) aaccaacGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGcc < 2:1989868/140‑1 (MQ=255) aacGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGaa < 2:2148751/140‑1 (MQ=255) aacGCCCACCAGCACCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGaa < 2:1277448/140‑1 (MQ=255) acGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAAc < 2:1690714/140‑1 (MQ=255) cGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACa > 1:468130/1‑140 (MQ=255) cGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACa > 1:357441/1‑140 (MQ=255) caccaGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCCCCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGAt < 2:303332/140‑1 (MQ=255) caccaGCATCCAGAACGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACGAAACGATTAGGCGCGTAACTCATAAACGGCAGCGCCGCTGCGATTGCCGTCAGAAACAGCAGCAGAGCCAGAAAAGGAt < 2:710150/140‑1 (MQ=255) ccaGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTa > 1:894956/1‑140 (MQ=255) ccaGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTa > 1:1929558/1‑140 (MQ=255) gCATCCGGATCGTTTGCGGCCACAGCTGCGTGAGATGGCGCCCCTCACCCGAAGCTAAACGATTAGGCGCGTAACTGACAATCGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCTATAACGGGATTAATa < 2:375196/140‑1 (MQ=255) gCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGATACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATa < 1:1091814/140‑1 (MQ=255) gCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATa < 1:648579/140‑1 (MQ=255) aTCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATa > 2:1141991/1‑140 (MQ=255) gTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATAc < 1:351580/130‑1 (MQ=255) gTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATAc > 2:351580/1‑130 (MQ=255) gTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGt < 2:360793/140‑1 (MQ=255) tttGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTc > 1:2037617/1‑140 (MQ=255) tttGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTc > 1:600654/1‑140 (MQ=255) ttGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCa < 2:1929558/140‑1 (MQ=255) gCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACg > 1:462057/1‑140 (MQ=255) gCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACg < 1:503924/140‑1 (MQ=255) gCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGa > 2:783200/1‑140 (MQ=255) ccACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGaa < 1:1233211/140‑1 (MQ=255) cacaGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGaaa > 2:810999/1‑140 (MQ=255) cacaGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGaaa > 2:697710/1‑140 (MQ=255) caGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAAtt > 1:972517/1‑140 (MQ=255) caGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAAtt > 1:1561479/1‑140 (MQ=255) cTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACt < 1:810999/140‑1 (MQ=255) agagATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTc < 2:514227/140‑1 (MQ=255) gagaTGACGCCCCTCCCCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTcc < 2:2066024/140‑1 (MQ=255) agaTGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCa < 1:652477/140‑1 (MQ=255) tGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCAccc > 1:2056019/1‑140 (MQ=255) tGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCAccc > 1:939456/1‑140 (MQ=255) gCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCtttt > 2:2060932/1‑140 (MQ=255) ccccTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCttttt < 2:939456/140‑1 (MQ=255) ccTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGt < 2:2037617/140‑1 (MQ=255) cTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTc > 2:316321/1‑127 (MQ=255) cTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTc < 1:316321/127‑1 (MQ=255) aaaCTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAg > 1:816931/1‑140 (MQ=255) tAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCTCCCTTTTTGTTTCAGGTAGTCgg < 2:1911590/140‑1 (MQ=255) aaaCGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGc < 2:1561479/140‑1 (MQ=255) aaCGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCa > 1:347245/1‑140 (MQ=255) aCGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAg > 1:2028107/1‑140 (MQ=255) cgcgTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACttttt > 1:479329/1‑140 (MQ=255) gATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAAATACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCa > 2:1817771/1‑140 (MQ=255) aCGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCt > 1:686258/1‑140 (MQ=255) gCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTcc > 2:975334/1‑140 (MQ=255) gCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTcc > 1:1648253/1‑140 (MQ=255) gCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTcc > 2:2089345/1‑140 (MQ=255) cAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCca < 2:468130/140‑1 (MQ=255) cAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCca > 1:1751635/1‑140 (MQ=255) cAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCca > 1:1752273/1‑140 (MQ=255) cAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGAGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCca > 1:512214/1‑140 (MQ=255) agcagcagaGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCaa < 2:1751635/140‑1 (MQ=255) | CAACCAACGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAA > NC_000913/2218388‑2218656 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |