Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,446,409	G→T	E766D (GAG→GAT)	tamB →	translocation and assembly module subunit TamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,446,409	0	G	T	100.0%	265.7 / NA	75	E766D (GAG→GAT)	tamB	translocation and assembly module subunit TamB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (39/36);  total (39/36)

GGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTG  >  NC_000913/4446274‑4446544                                                                                                                                        |                                                                                                                                        gggggCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGc                                                                                                                                     <  2:844570/140‑1 (MQ=255)  ggggCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCt                                                                                                                                    >  1:1206709/1‑140 (MQ=255)  ggggCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCt                                                                                                                                    >  1:386897/1‑140 (MQ=255)      ccGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCg                                                                                                                                <  1:581252/140‑1 (MQ=255)        gAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCa                                                                                                                              >  1:817949/1‑140 (MQ=255)          aGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAgg                                                                                                                            <  1:631430/140‑1 (MQ=255)          aGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAgg                                                                                                                            <  2:766505/140‑1 (MQ=255)           gggCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAggg                                                                                                                           <  1:397222/140‑1 (MQ=255)           gggCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAggg                                                                                                                           <  1:585562/140‑1 (MQ=255)           gggCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAggg                                                                                                                           <  1:1916149/140‑1 (MQ=255)              cGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAg                                                                                                                        <  1:431763/140‑1 (MQ=255)               gTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGt                                                                                                                       >  1:1854019/1‑140 (MQ=255)               gTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGt                                                                                                                       >  2:83827/1‑140 (MQ=255)                   ggtggtGAATCTCAACCGCTTCGTCCTCGCCATGCTGAAACCGTCTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCa                                                                                                                   <  2:75451/140‑1 (MQ=255)                   ggtggtGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCa                                                                                                                   <  2:1206709/140‑1 (MQ=255)                      ggtgAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCAccc                                                                                                                >  2:266055/1‑140 (MQ=255)                       gtgAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCt                                                                                                               >  2:1279923/1‑140 (MQ=255)                          aaTCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTc                                                                                                            <  2:817949/140‑1 (MQ=255)                          aaTCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTc                                                                                                            <  1:1918721/140‑1 (MQ=255)                               cAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGc                                                                                                       >  1:89965/1‑140 (MQ=255)                                 aCCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGt                                                                                                     <  1:1819524/140‑1 (MQ=255)                                 aCCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGt                                                                                                     <  1:1669030/140‑1 (MQ=255)                                 aCCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGt                                                                                                     >  1:969900/1‑140 (MQ=255)                                     cTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGt                                                                                                     >  1:47393/1‑136 (MQ=255)                                     cTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGt                                                                                                     <  2:47393/136‑1 (MQ=255)                                     cTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACg                                                                                                 >  1:323697/1‑140 (MQ=255)                                     cTTCGACCTCGCCATACTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGt                                                                                                     <  2:1554015/136‑1 (MQ=255)                                     cTTCGACCTCGCCATACTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGt                                                                                                     >  1:1554015/1‑136 (MQ=255)                                      ttCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGt                                                                                                <  2:386897/140‑1 (MQ=255)                                          aCCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAg                                                                                            >  2:1674234/1‑140 (MQ=255)                                                  aTGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCa                                                                                    <  1:1674234/140‑1 (MQ=255)                                                              tttATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGa                                                                        >  2:50845/1‑140 (MQ=255)                                                              tttATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGa                                                                        >  2:1813986/1‑140 (MQ=255)                                                              tttATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGa                                                                        >  1:1668358/1‑140 (MQ=255)                                                                               tCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTgg                                                       >  2:1792895/1‑140 (MQ=255)                                                                                  ggCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCACAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGt                                                    <  1:1222789/140‑1 (MQ=255)                                                                                   gCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGtt                                                   >  1:1823002/1‑140 (MQ=255)                                                                                   gCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGtt                                                   >  2:2050399/1‑140 (MQ=255)                                                                                   gCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGAGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGtt                                                   >  1:490879/1‑140 (MQ=255)                                                                                        cGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGa                                              >  2:2047555/1‑140 (MQ=255)                                                                                          gTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGaca                                            <  2:1854019/140‑1 (MQ=255)                                                                                            tacTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCTCCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACCCt                                          <  2:1599992/138‑1 (MQ=255)                                                                                            aTCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACt                                          <  1:1467621/140‑1 (MQ=255)                                                                                             tCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTg                                         >  2:1739907/1‑140 (MQ=255)                                                                                             tCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTg                                         >  1:167013/1‑140 (MQ=255)                                                                                                  aCGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCt                                    <  2:1823002/140‑1 (MQ=255)                                                                                                         aaGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGAcggcg                             >  2:777722/1‑140 (MQ=255)                                                                                                         aaGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGAcggcg                             >  1:1602894/1‑140 (MQ=255)                                                                                                         aaGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGAcggcg                             >  1:1056113/1‑140 (MQ=255)                                                                                                         aaGCGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGAcggcg                             >  1:1238137/1‑140 (MQ=255)                                                                                                            cGGACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGaa                          <  1:1792895/140‑1 (MQ=255)                                                                                                             ggACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAAt                         <  1:50845/140‑1 (MQ=255)                                                                                                             ggACGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTACCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAAt                         <  2:969900/140‑1 (MQ=255)                                                                                                               aCGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTg                       <  1:266055/140‑1 (MQ=255)                                                                                                               aCGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTg                       <  1:391979/140‑1 (MQ=255)                                                                                                               aCGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTg                       <  1:777722/140‑1 (MQ=255)                                                                                                                cGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGc                      >  1:394946/1‑140 (MQ=255)                                                                                                                cGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGc                      >  2:1062135/1‑140 (MQ=255)                                                                                                                cGTTGCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGc                      >  1:548625/1‑140 (MQ=255)                                                                                                                    gCCTGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTaa                  <  1:1963963/140‑1 (MQ=255)                                                                                                                       tGGGACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTaacaa               >  2:1458751/1‑140 (MQ=255)                                                                                                                         ggACACCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAAcc             <  1:1279923/140‑1 (MQ=255)                                                                                                                           acacCACGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGt           >  1:188820/1‑140 (MQ=255)                                                                                                                            caccacGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTg          <  2:89965/140‑1 (MQ=255)                                                                                                                            caccacGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTg          <  2:167013/140‑1 (MQ=255)                                                                                                                            caccacGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGCAACAACCGTg          <  2:548625/140‑1 (MQ=255)                                                                                                                             accacGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGc         >  1:1812331/1‑140 (MQ=255)                                                                                                                               cacGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTg          >  2:1830394/1‑137 (MQ=255)                                                                                                                               cacGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTg          <  1:1830394/137‑1 (MQ=255)                                                                                                                               cacGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCg       >  2:1890253/1‑140 (MQ=255)                                                                                                                               cacGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCg       >  2:1104784/1‑140 (MQ=255)                                                                                                                               cacGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCg       >  2:1308809/1‑140 (MQ=255)                                                                                                                                 cGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGaa     <  2:39861/140‑1 (MQ=255)                                                                                                                                 cGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGaa     >  2:442696/1‑140 (MQ=255)                                                                                                                                 cGAAAGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGaa     <  1:1949244/140‑1 (MQ=255)                                                                                                                                    aaGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTg  >  1:1679602/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        GGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTG  >  NC_000913/4446274‑4446544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑