Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,045,328	C→A	E192* (GAA→TAA)	ygfF ←	putative NAD(P)‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,045,328	0	C	A	100.0%	260.2 / NA	76	E192* (GAA→TAA)	ygfF	putative NAD(P)‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (38/38);  total (38/38)

GTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGA  >  NC_000913/3045192‑3045457                                                                                                                                         |                                                                                                                                  gTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGt                                                                                                                                <  2:1151357/140‑1 (MQ=255) gTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGt                                                                                                                                <  1:29568/140‑1 (MQ=255)  tGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGta                                                                                                                               <  2:785983/140‑1 (MQ=255)             gCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAAccc                                                                                                                    <  2:331261/140‑1 (MQ=255)                ttATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTgg                                                                                                                 >  1:766853/1‑140 (MQ=255)                     aCTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCcgca                                                                                                            <  2:529027/140‑1 (MQ=255)                      cTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCcgcac                                                                                                           >  2:409728/1‑140 (MQ=255)                       ttAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTataaata                                                                                                                         >  2:323034/1‑125 (MQ=255)                       ttAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTataaata                                                                                                                         <  1:323034/125‑1 (MQ=255)                       ttAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCcgcacg                                                                                                          >  1:632947/1‑140 (MQ=255)                        tagtagCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCcgcacgc                                                                                                         >  2:1345861/1‑140 (MQ=255)                        tagtagCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCcgcacgc                                                                                                         <  2:111094/140‑1 (MQ=255)                        tagtagCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCcgcacgc                                                                                                         <  1:39497/140‑1 (MQ=255)                         agtagCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCcgcacgca                                                                                                        >  1:1193897/1‑140 (MQ=255)                           tagCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCcg                                                                                                              <  2:455280/132‑1 (MQ=255)                           tagCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCcg                                                                                                              >  1:455280/1‑132 (MQ=255)                                aGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAAcg                                                                                                 <  1:385586/140‑1 (MQ=255)                                 gACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAAcgc                                                                                                >  1:744244/1‑140 (MQ=255)                                           tGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTg                                                                                      >  1:469150/1‑140 (MQ=255)                                            gcgcGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCATGCAGTTAACGCGGATCCCCTgc                                                                                     >  2:271458/1‑140 (MQ=255)                                             cgcgACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTgcg                                                                                    >  1:1124509/1‑140 (MQ=255)                                             cgcgACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTgcg                                                                                    >  2:1163964/1‑140 (MQ=255)                                                  ccTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCgcggcg                                                                               <  1:409728/140‑1 (MQ=255)                                                  ccTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCgcggcg                                                                               >  1:381947/1‑140 (MQ=255)                                                   ctctTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGa                                                                              <  2:534348/140‑1 (MQ=255)                                                       tctGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCATGCAGTTAACGCGGATCCCCTGCGCGGCGACTTc                                                                          <  1:271458/140‑1 (MQ=255)                                                            cTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCg                                                                     >  1:842913/1‑140 (MQ=255)                                                              gTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGAt                                                                   >  2:552299/1‑140 (MQ=255)                                                               tCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATa                                                                  >  2:804451/1‑140 (MQ=255)                                                               tCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATa                                                                  >  2:372675/1‑140 (MQ=255)                                                                ccaccaCGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAg                                                                 >  1:142269/1‑140 (MQ=255)                                                                ccaccaCGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAg                                                                 >  2:1268209/1‑140 (MQ=255)                                                                  accacGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGa                                                                              <  1:622681/125‑1 (MQ=255)                                                                  accacGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGa                                                                              >  2:622681/1‑125 (MQ=255)                                                                   ccacGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTcc                                                              <  1:121946/140‑1 (MQ=255)                                                                    cacGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCg                                                             <  2:1413843/140‑1 (MQ=255)                                                                    cacGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCg                                                             <  1:804451/140‑1 (MQ=255)                                                                    cacGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCg                                                             >  2:668689/1‑140 (MQ=255)                                                                    cacGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCGGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCg                                                             >  1:965058/1‑140 (MQ=255)                                                                      cGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCggt                                                           >  1:6798/1‑140 (MQ=255)                                                                         tGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCggtggt                                                        <  2:468980/140‑1 (MQ=255)                                                                          gCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGtt                                                       <  2:715231/140‑1 (MQ=255)                                                                          gCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGtt                                                       <  1:337328/140‑1 (MQ=255)                                                                          gCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGtt                                                       <  1:668689/140‑1 (MQ=255)                                                                          gCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGtt                                                       <  2:1302143/140‑1 (MQ=255)                                                                          gCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGtt                                                       <  1:605873/140‑1 (MQ=255)                                                                             tGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAAc                                                    >  2:462521/1‑140 (MQ=255)                                                                                     ttCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTa                                                      >  1:401804/1‑130 (MQ=255)                                                                                     ttCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTa                                                      <  2:401804/130‑1 (MQ=255)                                                                                     ttCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAAt                                            >  2:1407686/1‑140 (MQ=255)                                                                                     ttCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAAt                                            >  2:1137209/1‑140 (MQ=255)                                                                                     ttCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAAt                                            >  1:1446868/1‑140 (MQ=255)                                                                                      tCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTa                                                      <  2:757074/129‑1 (MQ=255)                                                                                      tCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTa                                                      >  1:757074/1‑129 (MQ=255)                                                                                       cGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCg                                          >  1:1018874/1‑140 (MQ=255)                                                                                         aCTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGcc                                        <  1:1163964/140‑1 (MQ=255)                                                                                          cTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGccc                                       >  2:354049/1‑140 (MQ=255)                                                                                          cTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGccc                                       >  1:172818/1‑140 (MQ=255)                                                                                                    tCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGc                             >  2:828378/1‑140 (MQ=255)                                                                                                       aCGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCcgc                          <  2:6798/140‑1 (MQ=255)                                                                                                       aCGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCcgc                          <  2:744244/140‑1 (MQ=255)                                                                                                       aCGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCcgc                          <  1:462521/140‑1 (MQ=255)                                                                                                            tCCAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAAt                     <  2:13720/140‑1 (MQ=255)                                                                                                              cAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCa                   >  2:459443/1‑140 (MQ=255)                                                                                                              cAGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCa                   <  2:762944/140‑1 (MQ=255)                                                                                                               aGGCTCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCaa                  <  2:842913/140‑1 (MQ=255)                                                                                                                   tCGCCGCCGCTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACata              <  2:172818/140‑1 (MQ=255)                                                                                                                     gccgccgcTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGcc                            <  2:311837/124‑1 (MQ=255)                                                                                                                     gccgccgcTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGcc                            >  1:311837/1‑124 (MQ=255)                                                                                                                     gccgccgcTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCcgc                          >  2:940120/1‑126 (MQ=255)                                                                                                                     gccgccgcTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCcgc                          <  1:940120/126‑1 (MQ=255)                                                                                                                      ccgccgcTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTc           >  2:94734/1‑140 (MQ=255)                                                                                                                        gccgcTGGCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTccc         <  1:1407686/140‑1 (MQ=255)                                                                                                                           gcgggCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTg      <  2:965058/137‑1 (MQ=255)                                                                                                                              ggCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCg   <  1:1345861/140‑1 (MQ=255)                                                                                                                               gCGTGCATTTAGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGa  >  2:388822/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                  GTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGA  >  NC_000913/3045192‑3045457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑