Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,233,852	C→T	A32V (GCT→GTT)	pgi →	glucose‑6‑phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,233,852	0	C	T	100.0%	268.6 / NA	76	A32V (GCT→GTT)	pgi	glucose‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/41);  total (35/41)

CTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGG  >  NC_000913/4233717‑4233987                                                                                                                                        |                                                                                                                                        cTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTaaa                                                                                                                                     <  1:723410/140‑1 (MQ=255)  ttCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAg                                                                                                                                    <  2:698270/140‑1 (MQ=255)   tCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGa                                                                                                                                   >  1:1185606/1‑140 (MQ=255)   tCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGa                                                                                                                                   >  1:161713/1‑140 (MQ=255)       aaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGc                                                                                                                               >  1:696393/1‑140 (MQ=255)       aaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGc                                                                                                                               <  2:1455726/140‑1 (MQ=255)            acaATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCg                                                                                                                          >  1:1029460/1‑140 (MQ=255)              aaTTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGtt                                                                                                                        <  1:927900/140‑1 (MQ=255)              aaTTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGtt                                                                                                                        <  2:245325/140‑1 (MQ=255)                   tcAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCt                                                                                                                   >  1:214312/1‑140 (MQ=255)                   tcAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCt                                                                                                                   >  2:194587/1‑140 (MQ=255)                      aaaTCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAg                                                                                                                <  1:792720/140‑1 (MQ=255)                       aaTCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGt                                                                                                               <  1:536554/140‑1 (MQ=255)                             aagaGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCg                                                                                                         <  1:788573/140‑1 (MQ=255)                             aagaGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCg                                                                                                         <  2:127508/140‑1 (MQ=255)                             aagaGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCg                                                                                                         >  2:206931/1‑140 (MQ=255)                                 gTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAAc                                                                                                     >  2:1252794/1‑140 (MQ=255)                                   aTTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCt                                                                                                   <  2:1416192/140‑1 (MQ=255)                                       cTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTcga                                                                                               <  1:194587/140‑1 (MQ=255)                                       cTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTcga                                                                                               >  1:1322255/1‑140 (MQ=255)                                       cTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTcga                                                                                               <  2:303633/140‑1 (MQ=255)                                       cTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTcga                                                                                               <  2:49776/140‑1 (MQ=255)                                          aTGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACTCTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTcga                                                                                               <  2:703254/137‑1 (MQ=255)                                          aTGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTcgacga                                                                                            >  1:492393/1‑140 (MQ=255)                                          aTGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTcgacga                                                                                            >  2:1684/1‑140 (MQ=255)                                          aTGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTcgacga                                                                                            >  1:510342/1‑140 (MQ=255)                                          aTGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTcgacga                                                                                            >  1:719073/1‑140 (MQ=255)                                          aTGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTcgacga                                                                                            >  2:505963/1‑140 (MQ=255)                                          aTGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTcga                                                                                               >  1:703254/1‑137 (MQ=255)                                            gAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATc                                                                                          >  2:986748/1‑140 (MQ=255)                                            gAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATc                                                                                          >  1:723197/1‑140 (MQ=255)                                             aaaaaCATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCa                                                                                         <  1:986748/140‑1 (MQ=255)                                               aaaCATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGa                                                                                       <  1:907379/140‑1 (MQ=255)                                               aaaCATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGa                                                                                       <  2:719073/140‑1 (MQ=255)                                               aaaCATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGa                                                                                       <  2:1322255/140‑1 (MQ=255)                                                 aCATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATg                                                                                     <  1:505963/140‑1 (MQ=255)                                                       aTCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCtggtg                                                                               >  2:1238619/1‑140 (MQ=255)                                                           aaCGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGAtt                                                                           <  2:767386/140‑1 (MQ=255)                                                           aaCGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGAtt                                                                           <  2:214312/140‑1 (MQ=255)                                                           aaCGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGAtt                                                                           <  2:1453748/140‑1 (MQ=255)                                                            aCGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTa                                                                          <  2:1185606/140‑1 (MQ=255)                                                             cGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTAc                                                                         >  1:1279131/1‑140 (MQ=255)                                                                  aCCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCaa                                                                    >  1:1119656/1‑140 (MQ=255)                                                                  aCCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCaa                                                                    >  2:973303/1‑140 (MQ=255)                                                                  aCCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCaa                                                                    >  1:720958/1‑140 (MQ=255)                                                                  aCCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCaa                                                                    >  2:540012/1‑140 (MQ=255)                                                                        gCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCg                                                              <  1:206931/140‑1 (MQ=255)                                                                         ccTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGc                                                             <  2:1308266/140‑1 (MQ=255)                                                                          cTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCa                                                            <  2:720958/140‑1 (MQ=255)                                                                          cTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCa                                                            >  1:3996/1‑140 (MQ=255)                                                                          cTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCa                                                            <  2:1014622/140‑1 (MQ=255)                                                                          cTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCa                                                            <  2:696393/140‑1 (MQ=255)                                                                           tGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCAt                                                           <  2:1473686/140‑1 (MQ=255)                                                                                       cAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGAc                                               >  1:702261/1‑140 (MQ=255)                                                                                       cAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGAc                                               >  2:577659/1‑140 (MQ=255)                                                                                            acacTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTgg                                          <  1:1238619/140‑1 (MQ=255)                                                                                            acacTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTgg                                          <  1:1252794/140‑1 (MQ=255)                                                                                               cTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGa                                       <  2:3996/140‑1 (MQ=255)                                                                                               cTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGa                                       >  1:702629/1‑140 (MQ=255)                                                                                                ttCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGaa                                      <  1:299412/140‑1 (MQ=255)                                                                                                ttCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGaa                                      <  2:702629/140‑1 (MQ=255)                                                                                                    atgaaatgaaAGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTa                                  >  2:186986/1‑140 (MQ=255)                                                                                                        aatgaaaGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCg                                        <  1:700701/130‑1 (MQ=255)                                                                                                        aatgaaaGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCg                                        >  2:700701/1‑130 (MQ=255)                                                                                                             aaGACGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTg                         >  2:319040/1‑140 (MQ=255)                                                                                                                 cGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGa                     <  2:702261/140‑1 (MQ=255)                                                                                                                 cGTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGa                     >  2:347253/1‑140 (MQ=255)                                                                                                                  gTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGaa                    <  2:510342/140‑1 (MQ=255)                                                                                                                  gTTACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGaa                    <  2:492393/140‑1 (MQ=255)                                                                                                                    tACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGAt                            <  2:687898/130‑1 (MQ=255)                                                                                                                    tACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGAt                            >  1:687898/1‑130 (MQ=255)                                                                                                                    tACGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACACTGGCGAAATTACAGGATCTGGCGAAag                  >  2:968117/1‑140 (MQ=255)                                                                                                                     aCGATCGCCGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAaga                 <  2:1279131/140‑1 (MQ=255)                                                                                                                            ccGATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGAt          >  1:1234272/1‑140 (MQ=255)                                                                                                                              gATCTTTTTGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCt        >  1:1195247/1‑140 (MQ=255)                                                                                                                                    tttGTTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTggcggg  <  2:1119656/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGG  >  NC_000913/4233717‑4233987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑