Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,392,311	C→A	intergenic (+162/‑49)	orn → / → glyV	oligoribonuclease/tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,392,311	0	C	A	100.0%	246.6 / NA	69	intergenic (+162/‑49)	orn/glyV	oligoribonuclease/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (35/34);  total (35/34)

GAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAA  >  NC_000913/4392176‑4392446                                                                                                                                        |                                                                                                                                        gAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTaa                                                                                                                                     >  1:818031/1‑140 (MQ=255) gAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTaa                                                                                                                                     >  1:131185/1‑140 (MQ=255) gAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTaa                                                                                                                                     >  1:1346219/1‑140 (MQ=255) gAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTaa                                                                                                                                     <  2:131185/140‑1 (MQ=255) gAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTaa                                                                                                                                     <  2:113614/140‑1 (MQ=255) gAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTaa                                                                                                                                     >  1:1471467/1‑140 (MQ=255)   aTCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAacg                                                                                                                                   >  2:1388401/1‑140 (MQ=255)          tGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGa                                                                                                                            <  2:1460353/140‑1 (MQ=255)          tGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGa                                                                                                                            <  1:988825/140‑1 (MQ=255)          tGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGa                                                                                                                            <  1:337896/140‑1 (MQ=255)             ggcggcgTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAAt                                                                                                                         <  2:818031/140‑1 (MQ=255)              gcggcgTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATg                                                                                                                        >  2:268288/1‑140 (MQ=255)                ggcgTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCg                                                                                                                      >  1:364432/1‑140 (MQ=255)                               cTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCa                                                                                                       <  1:268288/140‑1 (MQ=255)                               cTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCa                                                                                                       <  1:227488/140‑1 (MQ=255)                               cTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCa                                                                                                       <  2:1380025/140‑1 (MQ=255)                               cTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCa                                                                                                       <  1:588353/140‑1 (MQ=255)                                tAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAg                                                                                                         <  2:764282/137‑1 (MQ=255)                                tAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAg                                                                                                         >  1:764282/1‑137 (MQ=255)                                 aaaTTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCaaa                                                                                                     >  2:900188/1‑140 (MQ=255)                                  aaTTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCaaaa                                                                                                    <  2:1024377/140‑1 (MQ=255)                                  aaTTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCaaaa                                                                                                    >  2:145855/1‑140 (MQ=255)                                       aaCGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAag                                                                                               >  1:186177/1‑140 (MQ=255)                                          gTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAagtag                                                                                            <  2:498065/140‑1 (MQ=255)                                          gTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAagtag                                                                                            <  1:19050/140‑1 (MQ=255)                                             ttGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTAc                                                                                         <  1:1388401/140‑1 (MQ=255)                                              tGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGGAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTAcg                                                                                        <  2:511907/140‑1 (MQ=255)                                              tGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTAcg                                                                                        >  2:1000032/1‑140 (MQ=255)                                              tGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTAcg                                                                                        <  1:1000032/140‑1 (MQ=255)                                               gCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTAcgc                                                                                       <  1:1465373/140‑1 (MQ=255)                                                        ttCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGc                                                                              <  1:900188/140‑1 (MQ=255)                                                        ttCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGc                                                                              >  1:983779/1‑140 (MQ=255)                                                        ttCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGc                                                                              <  2:1346219/140‑1 (MQ=255)                                                          cAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTc                                                                            >  2:410561/1‑140 (MQ=255)                                                          cAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTc                                                                            >  1:966965/1‑140 (MQ=255)                                                             cACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGt                                                                         >  1:261870/1‑140 (MQ=255)                                                                ttGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGc                                                                              >  2:1166584/1‑132 (MQ=255)                                                                ttGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGc                                                                              <  1:1166584/132‑1 (MQ=255)                                                                  gagaTAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTa                                                                    >  1:773002/1‑140 (MQ=255)                                                                     aTAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTagag                                                                 >  2:572052/1‑140 (MQ=255)                                                                      tAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGc                                                                <  1:145855/140‑1 (MQ=255)                                                                          aaCGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGa                                                            >  1:496009/1‑140 (MQ=255)                                                                            cGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGAcc                                                          <  1:447596/140‑1 (MQ=255)                                                                             gCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCt                                                         >  2:385480/1‑140 (MQ=255)                                                                             gCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCt                                                         <  2:364432/140‑1 (MQ=255)                                                                               aaaaaaaaCTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTg                                                       >  2:596191/1‑140 (MQ=255)                                                                               aaaaaaaaCTTTTTTGGGGGGATGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTg                                                       >  1:1472167/1‑140 (MQ=255)                                                                                 aaaaaaCTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGcc                                                     <  2:1435850/140‑1 (MQ=255)                                                                                               gggggTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgc                                       <  2:626813/140‑1 (MQ=255)                                                                                                  ggTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAg                                    >  1:1322621/1‑140 (MQ=255)                                                                                                    ttGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGtt                                  >  2:1289962/1‑140 (MQ=255)                                                                                                     tGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTc                                 <  1:572052/140‑1 (MQ=255)                                                                                                       cAGAGGGAAAGATTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGa                               <  2:977867/140‑1 (MQ=255)                                                                                                                  aTTTCTCGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTcc                    <  2:983779/140‑1 (MQ=255)                                                                                                                      ctcGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTc                                 >  1:1117484/1‑123 (MQ=255)                                                                                                                      ctcGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTc                                 <  2:1117484/123‑1 (MQ=255)                                                                                                                      ctcGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCt                >  1:544532/1‑140 (MQ=255)                                                                                                                      ctcGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCt                >  1:422000/1‑140 (MQ=255)                                                                                                                        cGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTcc              >  1:18858/1‑140 (MQ=255)                                                                                                                        cGTATAATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTcc              >  1:383971/1‑140 (MQ=255)                                                                                                                           ataATGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaaa           <  2:143768/140‑1 (MQ=255)                                                                                                                             aaTGCGCCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAAt         <  2:186177/140‑1 (MQ=255)                                                                                                                                 cgcCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGa     >  1:456212/1‑140 (MQ=255)                                                                                                                                 cgcCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGa     >  2:123226/1‑140 (MQ=255)                                                                                                                                  gcCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGaa    <  1:385480/140‑1 (MQ=255)                                                                                                                                  gcCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGaa    <  1:209240/140‑1 (MQ=255)                                                                                                                                  gcCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGaa    <  1:1289962/140‑1 (MQ=255)                                                                                                                                  gcCTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGaa    <  2:966965/140‑1 (MQ=255)                                                                                                                                   ccTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGaaa   >  1:19997/1‑140 (MQ=255)                                                                                                                                    cTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGaaaa  >  1:933299/1‑140 (MQ=255)                                                                                                                                    cTCCAGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGaaaa  >  1:1260772/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        GAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAA  >  NC_000913/4392176‑4392446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑