Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,396,909	C→A	S282Y (TCC→TAC)	amiB →	N‑acetylmuramoyl‑L‑alanine amidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,396,909	0	C	A	100.0%	329.5 / NA	93	S282Y (TCC→TAC)	amiB	N‑acetylmuramoyl‑L‑alanine amidase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (49/44);  total (49/44)

TAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGC  >  NC_000913/4396772‑4397042                                                                                                                                          |                                                                                                                                        tAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACg                                                                                                                                       >  2:1113673/1‑140 (MQ=255)  aaaGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGt                                                                                                                                      >  2:1009515/1‑140 (MQ=255)       cGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGATTGGCGCTTACGTATggg                                                                                                                                 <  1:1009515/140‑1 (MQ=255)       cGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATggg                                                                                                                                 <  2:355902/140‑1 (MQ=255)       cGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATggg                                                                                                                                 >  1:1349007/1‑140 (MQ=255)       cGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATggg                                                                                                                                 <  1:1284134/140‑1 (MQ=255)        gTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGt                                                                                                                                <  1:51814/140‑1 (MQ=255)         ttttAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTg                                                                                                                               <  1:499690/140‑1 (MQ=255)         ttttAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTg                                                                                                                               >  1:610127/1‑140 (MQ=255)         ttttAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTg                                                                                                                               <  2:231491/140‑1 (MQ=255)          tttAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑c                                                                                                                            <  2:206163/140‑1 (MQ=255)          tttAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑c                                                                                                                            >  2:571813/1‑140 (MQ=255)              aCCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑ctctc                                                                                                                        >  1:952480/1‑140 (MQ=255)                ccGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTa                                                                                                                                     >  1:727568/1‑127 (MQ=255)                ccGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTa                                                                                                                                     <  2:727568/127‑1 (MQ=255)                  gTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAAc                                                                                                                    >  1:1286903/1‑140 (MQ=255)                   tGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGt                                                                                                                                <  2:220782/129‑1 (MQ=255)                   tGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGt                                                                                                                                >  1:220782/1‑129 (MQ=255)                   tGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAAcc                                                                                                                   >  2:921382/1‑140 (MQ=255)                     aCGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGt                                                                                                                 >  1:1406967/1‑140 (MQ=255)                          gATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCgcgc                                                                                                            <  1:35721/140‑1 (MQ=255)                          gATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTCACGTATGGGTG‑‑CTCTCTAACCGTCgcgc                                                                                                            >  2:1210589/1‑140 (MQ=255)                           aTTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGcc                                                                                                           <  2:1406967/140‑1 (MQ=255)                            ttACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCa                                                                                                          <  2:485788/140‑1 (MQ=255)                                  tATCTCGGTGATGGGGCGCAGTGTTGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTg                                                                                                    >  1:430619/1‑140 (MQ=255)                                    tctcGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGaa                                                                                                  >  2:84756/1‑140 (MQ=255)                                     ctcGGTGATGGGGCGCAGTGATGTGGCGCGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACCGTGaaa                                                                                                 >  1:1269528/1‑140 (MQ=255)                                     ctcGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTGCTCTCTCTAACCGTCGCGCCa                                                                                                          >  2:567793/1‑133 (MQ=255)                                     ctcGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTGCTCTCTCTAACCGTCGCGCCa                                                                                                          <  1:567793/133‑1 (MQ=255)                                     ctcGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGaaa                                                                                                 >  2:990991/1‑140 (MQ=255)                                     ctcGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGaaa                                                                                                 >  2:5561/1‑140 (MQ=255)                                      tcGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAAt                                                                                                >  2:1337609/1‑140 (MQ=255)                                        ggTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATgg                                                                                              >  2:1239073/1‑140 (MQ=255)                                         gTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGc                                                                                             >  2:1440189/1‑140 (MQ=255)                                         gTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGc                                                                                             >  1:442608/1‑140 (MQ=255)                                         gTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGc                                                                                             >  1:85974/1‑140 (MQ=255)                                         gTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGc                                                                                             >  1:270278/1‑140 (MQ=255)                                         gTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGc                                                                                             >  1:548386/1‑140 (MQ=255)                                          tGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGcc                                                                                            >  1:272887/1‑140 (MQ=255)                                          tGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGcc                                                                                            >  2:1321495/1‑140 (MQ=255)                                           gATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCa                                                                                           <  1:1113673/140‑1 (MQ=255)                                            aTGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAg                                                                                          <  1:1321495/140‑1 (MQ=255)                                            aTGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAg                                                                                          <  1:1241324/140‑1 (MQ=255)                                            aTGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAg                                                                                          <  2:756942/140‑1 (MQ=255)                                            aTGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAg                                                                                          <  2:862287/140‑1 (MQ=255)                                            aTGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAg                                                                                          <  2:610127/140‑1 (MQ=255)                                                  cgcAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAgctggct                                                                                    >  2:803500/1‑140 (MQ=255)                                                    cAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATT‑ACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGa                                                                                 >  1:1166053/1‑140 (MQ=255)                                                      gTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATT‑ACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGagc                                                                               <  2:1166053/140‑1 (MQ=255)                                                           gtgGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATTGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAgcagca                                                                           <  2:947634/140‑1 (MQ=255)                                                            tgGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAg                                                                                          <  2:1169057/124‑1 (MQ=255)                                                            tgGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAg                                                                                          >  1:1169057/1‑124 (MQ=255)                                                            tgGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCAc                                                                          >  2:488463/1‑140 (MQ=255)                                                            tgGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCAc                                                                          <  2:1019600/140‑1 (MQ=255)                                                                 cGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGaa                                                                     >  2:460386/1‑140 (MQ=255)                                                                  gTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGaaa                                                                    <  2:83355/140‑1 (MQ=255)                                                                      gCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGt                                                                <  1:990991/140‑1 (MQ=255)                                                                         aaaCGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCgg                                                             <  1:1440189/140‑1 (MQ=255)                                                                           aCGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAg                                                           <  2:166262/140‑1 (MQ=255)                                                                            cGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACa                                                                  >  1:37872/1‑132 (MQ=255)                                                                            cGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACa                                                                  <  2:37872/132‑1 (MQ=255)                                                                            cGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAg                                                           >  2:148703/1‑139 (MQ=255)                                                                            cGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAg                                                           <  1:148703/139‑1 (MQ=255)                                                                            cGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGG‑‑‑AGCACGAGAAACAGTCGGAgctgc                                                       >  2:1399146/1‑140 (MQ=255)                                                                             gCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAgct                                                         <  2:1310281/140‑1 (MQ=255)                                                                                    tCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCtgggt                                                  <  1:571813/140‑1 (MQ=255)                                                                                    tCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCtgggt                                                  <  2:1245922/140‑1 (MQ=255)                                                                                    tCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCtgggt                                                  >  1:109611/1‑140 (MQ=255)                                                                                        cGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTgggg                                              >  2:744227/1‑140 (MQ=255)                                                                                         gtgtCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGc                                             <  2:272887/140‑1 (MQ=255)                                                                                          tgtCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCg                                            <  1:5561/140‑1 (MQ=255)                                                                                           gtCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTATCCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGCAACAGTCAGAGCTGCTGGGAGGGGCgg                                           >  2:832335/1‑140 (MQ=255)                                                                                            tCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCggg                                          >  1:867841/1‑140 (MQ=255)                                                                                                     gCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCt                                 >  2:985810/1‑140 (MQ=255)                                                                                                       tGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTgg                               >  2:869066/1‑140 (MQ=255)                                                                                                         aTGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCg                             >  2:734177/1‑140 (MQ=255)                                                                                                          tGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGa                            >  1:103072/1‑140 (MQ=255)                                                                                                                aCCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAAcagtc                      <  2:1349007/140‑1 (MQ=255)                                                                                                                   gAACCGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAAcagtcagt                   >  2:25293/1‑140 (MQ=255)                                                                                                                       cGCAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGG‑‑‑AGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGAccc            <  1:1399146/140‑1 (MQ=255)                                                                                                                         cAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGAcc             >  2:629709/1‑140 (MQ=255)                                                                                                                         cAGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGAcc             <  2:1286903/140‑1 (MQ=255)                                                                                                                          ggTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGAccc            <  2:64048/139‑1 (MQ=255)                                                                                                                          aGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTATCCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGAccc            <  1:832335/140‑1 (MQ=255)                                                                                                                          aGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGAccc            <  1:84756/140‑1 (MQ=255)                                                                                                                          aGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGAccc            >  1:345729/1‑140 (MQ=255)                                                                                                                          aGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGAccc            <  1:1198629/140‑1 (MQ=255)                                                                                                                          aGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGAccc            <  1:488463/140‑1 (MQ=255)                                                                                                                          aGTGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGAccc            <  1:744227/140‑1 (MQ=255)                                                                                                                            tGCGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGGGATGTGCTGGCGAACAGTCAGTCTGACCCCt          >  2:1305685/1‑140 (MQ=255)                                                                                                                              cGACTGGCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTAt        >  2:1034629/1‑140 (MQ=255)                                                                                                                                   ggCGCTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAg   <  1:25293/140‑1 (MQ=255)                                                                                                                                    gcgcTTACGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGc  >  2:1479760/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        TAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTG‑‑CTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGC  >  NC_000913/4396772‑4397042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑