| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 646,747 | 0 | T | G | 26.8% | 74.2 / 23.7 | 41 | T255P (ACC→CCC) | citG | triphosphoribosyl‑dephospho‑CoA synthase |
| Reads supporting (aligned to +/- strand): ref base T (1/29); new base G (11/0); total (12/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.80e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTT > NC_000913/646608‑646884 | aaaTTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGt < 1:596211/140‑1 (MQ=255) gCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAAAG‑cccccc > 1:134097/1‑139 (MQ=255) cTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGaa < 2:432087/131‑1 (MQ=255) cTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGaa > 1:432087/1‑131 (MQ=255) ttAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCttt < 1:1061664/140‑1 (MQ=255) ttAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCttt < 1:469602/140‑1 (MQ=255) tAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCtttt < 1:222616/140‑1 (MQ=255) aaaTAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATG‑GCCCCctttt > 1:543337/1‑140 (MQ=255) atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATG‑CCCCCctttttccat > 1:52734/1‑135 (MQ=255) aaaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTGGGATG‑GCCCCCTTTTTTaataat > 2:222616/1‑140 (MQ=255) gtgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATG‑CCCCCCTTTTTCAATAAATTTTgc > 2:704099/1‑140 (MQ=255) tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATGCCCCCCTTTTTGAAAAAATttttcg > 2:320406/1‑138 (MQ=255) tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGAATG‑CCCCCCTTTTGCaataattattttg > 2:926636/1‑133 (MQ=255) tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATG‑CCCCCTTTTTGCAATAATGTTTgcg > 1:130602/1‑140 (MQ=255) tgCTAAAAACCAGGGAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTAGCAAG‑CCCCCTTTTTTTTAAAATGTTTtcg > 2:140981/1‑140 (MQ=255) gCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGAATA‑TCCCCCTTTTTTAATAATTTTTgttc > 2:1073085/1‑137 (MQ=255) gCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATG‑CCCCCCTTTTTCAATAATGTTTtcgc > 1:209697/1‑140 (MQ=255) cTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGcc < 1:105682/140‑1 (MQ=255) aaaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTc < 2:405951/140‑1 (MQ=255) aaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATG‑CCCCCCTTTTTTAATAATATTTGCGCCTcccg > 2:591074/1‑140 (MQ=255) cAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTc < 2:44958/134‑1 (MQ=255) cAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATG‑CCCCCCTTTTTTAATAATGTTTTTGCCTCgcgccg > 2:105682/1‑138 (MQ=255) cAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATG‑CCCCCCTTTTGCAATAATGTTTGCGCCTc > 1:44958/1‑134 (MQ=255) ggTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATG‑CCCCCCTTTTTCAATAATTTTTTCGCCCCCCGCTGTa > 1:655151/1‑140 (MQ=255) tAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGc < 1:67959/140‑1 (MQ=255) gATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAgc < 1:884359/140‑1 (MQ=255) aGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATG‑CCCCCTTTTTTTAATAATTTTTTCGCCTCGCGCTGTAGGCAgcgccg > 1:714134/1‑138 (MQ=255) aGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGAAAATCGAGATCGGCGGGGGTTGGAATG‑CCCCCCTTTTTCAATTATTATTTCTCCCCGCCCCGTAGCCAGCGCAg > 1:53022/1‑140 (MQ=255) gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTGGGATG‑CCCCCCTTTTTTAATAAAATTTTTGCCCCCCCCCGGAGCCAGCGCCgg > 2:1002142/1‑140 (MQ=255) gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATG‑CCCCCCTTTTTCAATAATGTTTTCGCCTCCCGCTGTAGCCAGCGCAgg > 1:1027356/1‑140 (MQ=255) gTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATG‑GCCCCCTTTTTCAATAATGTTTTCGCCTCCCGCTGTAGCCAGCGCAAGcccc > 2:884359/1‑140 (MQ=255) gTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATG‑CCCCCCTTTTTTAATAATTTTTTTGCCCCGCGCTGTAGGCAGCGCCGGcccc > 2:971501/1‑140 (MQ=255) tCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATG‑CCCCCCTTTTTCAATAATTTTTTCGCCCCGCGCTGTAGCCAGCGCAGGccccc > 1:124855/1‑140 (MQ=255) cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGAATG‑GCCCCCTTTTTTTATAAAGTTTTCGCCCCGCGCCGTGGCCAGCGCCGGcccccc > 2:595857/1‑140 (MQ=255) tGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCa < 2:130602/140‑1 (MQ=255) cgccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGTCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcg < 1:13262/140‑1 (MQ=255) ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcg < 1:1002142/140‑1 (MQ=255) ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcg < 1:591074/140‑1 (MQ=255) ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcg < 2:603609/140‑1 (MQ=255) ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcg < 1:263508/140‑1 (MQ=255) ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCGTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcg < 1:563566/140‑1 (MQ=255) aCTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATg < 2:855256/140‑1 (MQ=255) cTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGc < 1:192602/140‑1 (MQ=255) tCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGAt < 2:544357/140‑1 (MQ=255) tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGcc < 2:527047/140‑1 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCa < 2:543337/140‑1 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCa < 2:309838/140‑1 (MQ=255) tCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCAt < 2:688437/140‑1 (MQ=255) tCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCAt < 1:1073085/140‑1 (MQ=255) aCTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt < 1:840545/140‑1 (MQ=255) gagaTAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCa < 1:294593/140‑1 (MQ=255) gagaTAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCa < 1:971501/140‑1 (MQ=255) aTCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTAt < 2:209697/140‑1 (MQ=255) aTCGGCGGGGGGTCGGATG‑CCCCCCTTTTTCAATAAAGTTTTGGCCTCGCCCTGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCCCCGTGCAACGTTGGGGTCCCCGGTGGTCGCCCTCTCGAGGGGCAAGGGATCGAGCa > 2:444991/1‑140 (MQ=255) aTCGGCGGGGGGTCGAATG‑CCCCCCTTTTTCAATAATTTTTTCGCCTCGCGCTGTAGCCCGCGCCGGCCCCCCCCCCCCCCCCCCGCTGCAACGTTGGTATCGCCGGTGGTCGCCCTCAGTAGGAGCAAGGGATCGAGCa > 2:768998/1‑140 (MQ=255) ggTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGtt < 2:655151/140‑1 (MQ=255) | AAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTT > NC_000913/646608‑646884 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |