Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,269,117	C→G	intergenic (+98/+51)	kdsA → / ← ldrA	3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase/small toxic polypeptide LdrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,269,117	0	C	G	100.0%	171.7 / NA	50	intergenic (+98/+51)	kdsA/ldrA	3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase/small toxic polypeptide LdrA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (27/23);  total (27/23)

GTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTG  >  NC_000913/1268984‑1269229                                                                                                                                      |                                                                                                                 gTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGttt                                                                                                            >  1:43366/1‑140 (MQ=255)    aaaGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCgg                                                                                                         >  1:1046184/1‑140 (MQ=255)      aGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGgc                                                                                                       >  1:853464/1‑140 (MQ=255)         tttCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGgcgc                                                                                                     >  1:767429/1‑139 (MQ=255)         tttCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGgcgc                                                                                                     <  2:767429/139‑1 (MQ=255)           tCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCata                                                                                                  >  1:229413/1‑140 (MQ=255)           tCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCata                                                                                                  >  1:364522/1‑140 (MQ=255)           tCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCata                                                                                                  >  2:243845/1‑140 (MQ=255)           tCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCata                                                                                                  >  2:507806/1‑140 (MQ=255)                 aaCTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCa                                                                                            <  1:1040914/140‑1 (MQ=255)                      gATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACg                                                                                       >  2:395953/1‑140 (MQ=255)                             gCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCt                                                                                >  1:869222/1‑140 (MQ=255)                                  tAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGc                                                                           <  2:884155/140‑1 (MQ=255)                                  tAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGc                                                                           >  2:759591/1‑140 (MQ=255)                                     tCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCg                                                                        >  1:759048/1‑140 (MQ=255)                                         ttttGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGc                                                                           <  2:665451/133‑1 (MQ=255)                                         ttttGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGc                                                                           >  1:665451/1‑133 (MQ=255)                                         ttttGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGtttt                                                                    <  2:853464/140‑1 (MQ=255)                                            tGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTGATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGt                                                                       >  1:1095027/1‑134 (MQ=255)                                            tGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGt                                                                       >  1:1066692/1‑134 (MQ=255)                                            tGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGt                                                                       <  2:1095027/134‑1 (MQ=255)                                            tGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGt                                                                       <  2:1066692/134‑1 (MQ=255)                                                   aaaaTAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCACGTTTTCACCTTACtt                                                          <  2:996914/140‑1 (MQ=255)                                                         aaGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGtt                                                    <  2:759048/140‑1 (MQ=255)                                                             aTTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGc                                                <  1:759591/140‑1 (MQ=255)                                                                aGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGccac                                             <  2:869222/140‑1 (MQ=255)                                                                 gCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGccacc                                            >  1:1048734/1‑140 (MQ=255)                                                                 gCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGccacc                                            <  1:243845/140‑1 (MQ=255)                                                                 gCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGccacc                                            >  2:160097/1‑140 (MQ=255)                                                                      cTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGt                                                     >  1:875712/1‑126 (MQ=255)                                                                      cTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGt                                                     <  2:875712/126‑1 (MQ=255)                                                                      cTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTg                                       >  1:413547/1‑140 (MQ=255)                                                                      cTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTg                                       >  2:450561/1‑140 (MQ=255)                                                                        gCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGAc                                     >  1:907202/1‑140 (MQ=255)                                                                         cGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGAc                                     >  2:535741/1‑139 (MQ=255)                                                                         cGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGAc                                     <  1:535741/139‑1 (MQ=255)                                                                               gACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCt                              <  1:395953/140‑1 (MQ=255)                                                                                aCTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTg                             <  2:364522/140‑1 (MQ=255)                                                                                aCTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTg                             <  1:384285/140‑1 (MQ=255)                                                                                  ttttttCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCg                           <  1:450561/140‑1 (MQ=255)                                                                                          tGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTaataat                   >  1:931971/1‑140 (MQ=255)                                                                                          tGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTaataat                   >  2:861071/1‑140 (MQ=255)                                                                                           gggTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCCCCAGCTGACAATCGCTGCGGTAATAAtt                  <  2:537049/140‑1 (MQ=255)                                                                                           gggTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAAtt                  <  2:413547/140‑1 (MQ=255)                                                                                           gggTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAAtt                  >  2:227718/1‑140 (MQ=255)                                                                                              tGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTccc               >  1:269803/1‑140 (MQ=255)                                                                                              tGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTccc               >  2:266900/1‑140 (MQ=255)                                                                                              tGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTccc               >  2:976649/1‑140 (MQ=255)                                                                                                ccGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGc             <  2:1048734/140‑1 (MQ=37)                                                                                                         aaaaaGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCgg    <  2:907202/140‑1 (MQ=17)                                                                                                           aaaGGCCGCAGGCTGTTACCCCTGCGGGCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGtg  <  2:1046184/140‑1 (MQ=17)                                                                                                                                      |                                                                                                                 GTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTG  >  NC_000913/1268984‑1269229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑