Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,351,376	G→T	intergenic (‑337/+31)	fabI ← / ← ycjD	enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,351,376	0	G	T	100.0%	181.3 / NA	52	intergenic (‑337/+31)	fabI/ycjD	enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/28);  total (24/28)

GGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCA  >  NC_000913/1351239‑1351501                                                                                                                                          |                                                                                                                              ggACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCt                                                                                                                             >  2:575581/1‑140 (MQ=255)         ttGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCt                                                                                                                     <  2:721848/140‑1 (MQ=255)         ttGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCt                                                                                                                     <  1:558004/140‑1 (MQ=255)            tgCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTctg                                                                                                                  >  2:1029391/1‑138 (MQ=255)                 atatTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGc                                                                                                             >  2:193187/1‑140 (MQ=255)                 atatTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGc                                                                                                             >  2:307941/1‑140 (MQ=255)                      gTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCt                                                                                                        >  1:660317/1‑140 (MQ=255)                        tACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCg                                                                                                      <  1:193187/140‑1 (MQ=255)                        tACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCg                                                                                                      <  1:845648/140‑1 (MQ=255)                              aGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCttt                                                                                                <  1:10608/140‑1 (MQ=255)                              aGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAAACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCttt                                                                                                >  1:9574/1‑140 (MQ=255)                                  acaaacaGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAgg                                                                                            <  1:281344/140‑1 (MQ=255)                                          gCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAggga                                                                                    >  1:522940/1‑139 (MQ=255)                                           cTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTg                                                                                   >  2:374126/1‑140 (MQ=255)                                                    ccccTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGAc                                                                                 <  1:588230/133‑1 (MQ=255)                                                    ccccTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGAc                                                                                 >  2:588230/1‑133 (MQ=255)                                                    ccccTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGt                                                                          >  1:305237/1‑140 (MQ=255)                                                    ccccTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGt                                                                          >  1:257824/1‑140 (MQ=255)                                                      ccTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTc                                                                        <  1:1029391/140‑1 (MQ=255)                                                      ccTAACCCTTTCATGGCGATGGCTGGGACGGTTCAAACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTc                                                                        <  2:9574/140‑1 (MQ=255)                                                             cTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCt                                                                 <  2:257824/140‑1 (MQ=255)                                                              tttCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTg                                                                >  2:41259/1‑140 (MQ=255)                                                              tttCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTg                                                                <  2:268644/140‑1 (MQ=255)                                                               ttCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGc                                                               >  2:703000/1‑140 (MQ=255)                                                                tCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCa                                                              <  2:588293/140‑1 (MQ=255)                                                                tCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCa                                                              <  2:240353/140‑1 (MQ=255)                                                                   tGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGt                                                                     <  2:958989/130‑1 (MQ=255)                                                                   tGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGt                                                                     >  1:958989/1‑130 (MQ=255)                                                                   tGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGaa                                                           <  1:1114002/140‑1 (MQ=255)                                                                    ggCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAat                                                          >  1:40190/1‑140 (MQ=255)                                                                     gCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAata                                                         <  2:111985/140‑1 (MQ=255)                                                                     gCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAata                                                         <  2:522940/140‑1 (MQ=255)                                                                         tGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATtct                                                     >  2:593901/1‑140 (MQ=255)                                                                               ggACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCa                                               <  2:40190/140‑1 (MQ=255)                                                                                   ggTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGt                                           <  1:374126/140‑1 (MQ=255)                                                                                     ttCAGATCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGtct                                         >  2:779163/1‑140 (MQ=255)                                                                                     ttCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGtct                                         >  2:590155/1‑140 (MQ=255)                                                                                     ttCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGtct                                         <  2:165199/140‑1 (MQ=255)                                                                                        gagacaggCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTctcct                                      <  1:763145/133‑1 (MQ=255)                                                                                               gCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTAca                               >  2:111303/1‑140 (MQ=255)                                                                                               gCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTAca                               >  2:350839/1‑140 (MQ=255)                                                                                                 cGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTAcaat                             <  1:80478/140‑1 (MQ=255)                                                                                                    atatTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTAcaatcaa                          <  1:779163/140‑1 (MQ=255)                                                                                                     tatTCTCCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTAcaatcaat                         <  1:787308/140‑1 (MQ=255)                                                                                                          tcCAGCACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTTTGTTTCCGTAGAAAATTCTCCAGCCCCGTCCACTCCTTAcaatcactttctc                    >  2:763145/1‑133 (MQ=255)                                                                                                               cACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTc               >  2:689379/1‑140 (MQ=255)                                                                                                               cACCGTCTCCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCCCCAGAAAATTCTCCAACACCGCCTCCCCATTTCAATCAATCTCGTTATTc               >  2:536116/1‑140 (MQ=255)                                                                                                                    tctcCATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGaa          >  2:796631/1‑140 (MQ=255)                                                                                                                       ccATGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACg       <  1:307941/140‑1 (MQ=255)                                                                                                                          tGTTTTCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACgcag    <  1:593901/140‑1 (MQ=255)                                                                                                                            ttttCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACgcagca  <  1:703000/140‑1 (MQ=255)                                                                                                                            ttttCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACgcagca  <  2:1027418/140‑1 (MQ=255)                                                                                                                            ttttCACCACAACATCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACgcagca  <  2:660317/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                              GGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCA  >  NC_000913/1351239‑1351501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑