Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,703,301	G→T	S336S (TCC→TCA)	ydgJ ←	putative oxidoreductase YdgJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,703,301	0	G	T	100.0%	158.1 / NA	45	S336S (TCC→TCA)	ydgJ	putative oxidoreductase YdgJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/18);  total (27/18)

TCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCGCATAGTAAGCCGGATAATTCCCAGGCACC  >  NC_000913/1703168‑1703427                                                                                                                                      |                                                                                                                               tCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCg                                                                                                                          >  1:1055197/1‑140 (MQ=255)      aaaTGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGcc                                                                                                                     <  2:1055197/140‑1 (MQ=255)         tGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAg                                                                                                                  <  1:162536/140‑1 (MQ=255)           gCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCt                                                                                                                >  1:854748/1‑140 (MQ=255)                ccTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCtcaatc                                                                                                           >  1:932445/1‑140 (MQ=255)                  tCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCtcaatcaa                                                                                                         <  1:1055066/140‑1 (MQ=255)                     gcgcTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTc                                                                                                      >  2:320618/1‑140 (MQ=255)                           aGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCtcaatc                                                                                                           <  2:313973/129‑1 (MQ=255)                           aGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCtcaatc                                                                                                           >  1:313973/1‑129 (MQ=255)                           aGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTAc                                                                                                >  2:1098501/1‑140 (MQ=255)                           aGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTAc                                                                                                >  1:1002931/1‑140 (MQ=255)                                   cgcgCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTg                                                                                        >  2:855534/1‑140 (MQ=255)                                                                 tAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCAt                                                          <  1:1013594/140‑1 (MQ=255)                                                                 tAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCAt                                                          <  1:27586/140‑1 (MQ=255)                                                                 tAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCAt                                                          <  1:855534/140‑1 (MQ=255)                                                                           gatggTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCTATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTaa                                                >  1:149349/1‑140 (MQ=255)                                                                           gatggTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTaa                                                <  2:932445/140‑1 (MQ=255)                                                                           gatggTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTaa                                                <  1:1098501/140‑1 (MQ=255)                                                                               gTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCa                                            >  2:150598/1‑140 (MQ=255)                                                                                tGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCAt                                           >  1:654163/1‑140 (MQ=255)                                                                                tGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCAt                                           >  1:317901/1‑140 (MQ=255)                                                                                       gCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAAt                                    <  1:320618/140‑1 (MQ=255)                                                                                          ccatccatTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCAt                                           <  1:1005704/130‑1 (MQ=255)                                                                                          ccatccatTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCAt                                           >  2:1005704/1‑130 (MQ=255)                                                                                          ccatccatTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGc                                 >  2:843843/1‑140 (MQ=255)                                                                                          ccatccatTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGc                                 >  1:622422/1‑140 (MQ=255)                                                                                           catccatTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGcc                                >  2:522336/1‑140 (MQ=255)                                                                                            atccatTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAAt                                    <  1:720914/135‑1 (MQ=255)                                                                                            atccatTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAAt                                    >  2:720914/1‑135 (MQ=255)                                                                                            atccatTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCg                               >  2:590029/1‑140 (MQ=255)                                                                                            atccatTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCg                               <  2:622422/140‑1 (MQ=255)                                                                                             tccatTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCGc                              >  2:250714/1‑140 (MQ=255)                                                                                             tccatTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCGc                              >  1:1037460/1‑140 (MQ=255)                                                                                             tccatTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCGc                              >  2:927298/1‑140 (MQ=255)                                                                                                 tttttCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCGCATAg                          >  2:652709/1‑140 (MQ=255)                                                                                                  ttttCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCGCATAGt                         >  2:1021328/1‑140 (MQ=255)                                                                                                     tCATGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCGCATAGTAAg                      <  1:843843/140‑1 (MQ=255)                                                                                                       aTGCAAGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCGCATAGTAAGcc                    >  2:1087198/1‑140 (MQ=255)                                                                                                           aaGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCg                               <  1:863569/125‑1 (MQ=255)                                                                                                           aaGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCg                               >  2:863569/1‑125 (MQ=255)                                                                                                           aaGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCGCATAGTAAGCCGGAt                <  2:1002931/140‑1 (MQ=255)                                                                                                           aaGGCACAAAGTCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCGCATAGTAAGCCGGAt                >  2:843596/1‑140 (MQ=255)                                                                                                                      tCGCGCGATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCGCATAGTAAGCCGGATAATTCCCAGGc     <  2:854748/140‑1 (MQ=255)                                                                                                                       cgcgcgATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCGCATAGTAAGCCGGATAATTCCCAGGCa    <  2:961875/140‑1 (MQ=255)                                                                                                                         cgcgATGTTTGGCTGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCGCATAGTAAGCCGGATAATTCCCAGGCAcc  >  1:865033/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                               TCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCGCATAGTAAGCCGGATAATTCCCAGGCACC  >  NC_000913/1703168‑1703427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑