Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,727,199 C→T L181F (CTC→TTC)  nemA → N‑ethylmaleimide reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,727,1990CT93.8% 140.3 / 0.4 48L181F (CTC→TTC) nemAN‑ethylmaleimide reductase
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (23/22);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

TGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGCTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAG  >  NC_000913/1727066‑1727331
                                                                                                                                     |                                                                                                                                    
tGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCAct                                                                                                                                <  2:1029452/140‑1 (MQ=255)
     aTGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCt                                                                                                                           >  1:625310/1‑140 (MQ=255)
            ggCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTCTTGATCTGGTAGAGTTCCACTCTGCTCACGGtt                                                                                                                    >  2:935233/1‑140 (MQ=255)
             gCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTa                                                                                                                   >  2:954178/1‑140 (MQ=255)
                   cGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTtgct                                                                                                             <  1:844143/140‑1 (MQ=255)
                        tGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATc                                                                                                        <  2:16467/140‑1 (MQ=255)
                         gAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCa                                                                                                       <  2:195632/140‑1 (MQ=255)
                          aaaCATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGCTCCACTCTGCTCACGGTTATTTGCTGCATCAg                                                                                                      <  1:1105015/140‑1 (MQ=255)
                            aCATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGtt                                                                                                    <  2:192626/140‑1 (MQ=255)
                              aTCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTcc                                                                                                  >  2:882232/1‑140 (MQ=255)
                               tCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAg                                                                                                      >  2:858821/1‑135 (MQ=255)
                               tCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAg                                                                                                      <  1:858821/135‑1 (MQ=255)
                                ccATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCtt                                                                                                <  2:699072/140‑1 (MQ=255)
                                ccATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCtt                                                                                                <  1:954178/140‑1 (MQ=255)
                                 cATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCttt                                                                                               >  2:874864/1‑140 (MQ=255)
                                 cATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCttt                                                                                               <  1:254475/140‑1 (MQ=255)
                                          tGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCcttctt                                                                                      >  1:902397/1‑140 (MQ=255)
                                            cgcTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTCTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCa                                                                                    <  1:935233/140‑1 (MQ=255)
                                              cTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCaaa                                                                                  >  1:871870/1‑140 (MQ=255)
                                                            gaTTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCa                                                                                    <  2:1108152/124‑1 (MQ=255)
                                                            gaTTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCa                                                                                    >  1:1108152/1‑124 (MQ=255)
                                                              ttCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGAt                                                                     >  2:969967/1‑137 (MQ=255)
                                                              ttCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGAt                                                                     <  1:969967/137‑1 (MQ=255)
                                                              ttCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAg                                                                  <  2:505921/140‑1 (MQ=255)
                                                                  aGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTAcg                                                              >  1:543147/1‑140 (MQ=255)
                                                                        cGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCa                                                        >  1:491435/1‑140 (MQ=255)
                                                                         gTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGAt                                                                     >  2:408790/1‑126 (MQ=255)
                                                                         gTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGAt                                                                     <  1:408790/126‑1 (MQ=255)
                                                                          tCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGc                                                      <  2:378939/140‑1 (MQ=255)
                                                                                  ttCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGaaaa                                              >  1:588886/1‑140 (MQ=255)
                                                                                        cAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCgcgc                                        <  2:210652/140‑1 (MQ=255)
                                                                                           gCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACg                                     >  1:716605/1‑140 (MQ=255)
                                                                                           gCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACg                                     >  1:334481/1‑140 (MQ=255)
                                                                                             cATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGTGGCAGCGTGGAAAATCGCGCACGtt                                   <  2:902397/140‑1 (MQ=255)
                                                                                              aTTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGttt                                  <  2:907112/140‑1 (MQ=255)
                                                                                                     aCGCGCGTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTg                           >  2:44001/1‑140 (MQ=255)
                                                                                                      cgcgcgTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTgg                          >  1:263092/1‑140 (MQ=255)
                                                                                                          cgTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGt                      >  1:1051955/1‑140 (MQ=255)
                                                                                                          cgTGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGt                      >  2:662559/1‑140 (MQ=255)
                                                                                                          cgTGAAGCCGGTTTTGATCTGGTAGAGCTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGt                      >  1:231502/1‑140 (MQ=255)
                                                                                                            tGAAGCCGGTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTgg                    >  1:210205/1‑140 (MQ=255)
                                                                                                              aaGCCGGTTTTGATCTGGTAGAGCTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTc                  <  2:231502/140‑1 (MQ=255)
                                                                                                                   ggTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGc             <  2:716605/140‑1 (MQ=255)
                                                                                                                   ggTTTTGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGc             <  1:662559/140‑1 (MQ=255)
                                                                                                                     ttttGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCg           <  2:968679/140‑1 (MQ=255)
                                                                                                                     ttttGATCTGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCg           >  1:791341/1‑140 (MQ=255)
                                                                                                                             tGGTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTgaa   <  2:1051955/140‑1 (MQ=255)
                                                                                                                              ggTAGAGTTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTgaag  >  2:44057/1‑140 (MQ=255)
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TGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGCTCCACTCTGCTCACGGTTATTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAG  >  NC_000913/1727066‑1727331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑