Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	614,751	G→T	E199* (GAA→TAA)	entF →	apo‑serine activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	614,751	0	G	T	100.0%	181.6 / NA	51	E199* (GAA→TAA)	entF	apo‑serine activating enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/24);  total (27/24)

ACTGCACATGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCA  >  NC_000913/614626‑614881                                                                                                                              |                                                                                                                                   aCTGCACATGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCaa                                                                                                                      >  2:366229/1‑140 (MQ=255)     cacaTGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTgc                                                                                                                  >  2:135708/1‑140 (MQ=255)      acaTGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTgcc                                                                                                                 >  2:112656/1‑140 (MQ=255)       caTGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTgccg                                                                                                                >  2:745404/1‑140 (MQ=255)       caTGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTgccg                                                                                                                >  2:641073/1‑140 (MQ=255)         tGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTgccgcc                                                                                                              <  2:129592/140‑1 (MQ=255)            cTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTgccgccgcc                                                                                                           <  1:366229/140‑1 (MQ=255)             tGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGccgccc                                                                                                          >  1:173191/1‑140 (MQ=255)               cGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTgccgccgc                                                                                                            >  2:770054/1‑136 (MQ=255)               cGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTgccgccgc                                                                                                            <  1:770054/136‑1 (MQ=255)               cGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGccgcccgc                                                                                                        >  1:349766/1‑140 (MQ=255)               cGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGccgcccgc                                                                                                        >  2:23016/1‑140 (MQ=255)                gTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCcgcg                                                                                                       <  2:672786/140‑1 (MQ=255)                 tGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGt                                                                                                      >  2:839212/1‑140 (MQ=255)                    cGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCAc                                                                                                   >  2:976982/1‑140 (MQ=255)                      aaCCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACtt                                                                                                 >  1:899463/1‑140 (MQ=255)                      aaCCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACtt                                                                                                 <  2:476560/140‑1 (MQ=255)                      aaCCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACtt                                                                                                 >  2:383615/1‑140 (MQ=255)                      aaCCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACtt                                                                                                 >  2:83166/1‑140 (MQ=255)                             gCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCgg                                                                                          <  1:839212/140‑1 (MQ=255)                                       ccATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCAc                                                                                       <  1:592344/133‑1 (MQ=255)                                       ccATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCAc                                                                                       >  2:592344/1‑133 (MQ=255)                                         aTTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCgg                                                                              >  2:718630/1‑140 (MQ=255)                                            tACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGgc                                                                           >  2:406166/1‑140 (MQ=255)                                               gCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCa                                                                        <  2:173191/140‑1 (MQ=255)                                                  tttCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAg                                                                       <  2:176074/138‑1 (MQ=255)                                                  tttCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAg                                                                       >  1:176074/1‑138 (MQ=255)                                                   ttCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAgcgc                                                                    <  1:23016/140‑1 (MQ=255)                                                      gCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTc                                                                 <  2:923743/140‑1 (MQ=255)                                                      gCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTc                                                                 <  1:718630/140‑1 (MQ=255)                                                        tGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCgg                                                               <  2:899463/140‑1 (MQ=255)                                                                    agagTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTgc                                                   >  2:517249/1‑140 (MQ=255)                                                                    agagTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTgc                                                   >  1:180556/1‑140 (MQ=255)                                                                     gagTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTc                                                                 <  1:1072340/125‑1 (MQ=255)                                                                     gagTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTc                                                                 >  2:1072340/1‑125 (MQ=255)                                                                       gTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGcc                                                <  1:487723/140‑1 (MQ=255)                                                                       gTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGcc                                                >  2:1053820/1‑140 (MQ=255)                                                                       gTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCACAGCGCCTCGGCAGATATCCTGCGcc                                                <  1:406166/140‑1 (MQ=255)                                                                       gTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCACCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGcc                                                >  2:1005625/1‑140 (MQ=255)                                                                       gTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCACCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGcc                                                <  1:1005625/140‑1 (MQ=255)                                                                          ccAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGa                                             >  2:853719/1‑140 (MQ=255)                                                                          ccAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGa                                             <  1:135708/140‑1 (MQ=255)                                                                             gCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAAc                                          <  1:641073/140‑1 (MQ=255)                                                                               aaTACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTg                                        <  2:683200/140‑1 (MQ=255)                                                                                    cgcgAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAAtt                                   <  1:517249/140‑1 (MQ=255)                                                                                      cgAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTa                                 >  2:389110/1‑140 (MQ=255)                                                                                                      cAGCGTGATGCGGCATTCTGGGCATAACAGCGTAGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGAGCACCGCCTCGGCAGATATTCTGCGCCTGAAACTGGTATTTACCGACGTGGCATTcaa                 >  1:564169/1‑138 (MQ=255)                                                                                                         cGTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCa              <  1:383615/140‑1 (MQ=255)                                                                                                          gTGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAg             <  1:905346/140‑1 (MQ=255)                                                                                                           tGATGCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGc            >  2:640430/1‑140 (MQ=255)                                                                                                               gCGGCATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGc        >  2:644326/1‑140 (MQ=255)                                                                                                                   cATTCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACg    <  1:45836/140‑1 (MQ=255)                                                                                                                     ttCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCa  <  2:64514/140‑1 (MQ=255)                                                                                                                     ttCTGGGCATAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCa  <  2:180556/140‑1 (MQ=255)                                                                                                                              |                                                                                                                                   ACTGCACATGGCTGCGTGGCGAACCAACGCCTGCTTCGCCATTTACGCCTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCA  >  NC_000913/614626‑614881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑