Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,053,832	C→A	L64I (CTC→ATC)	shiA →	shikimate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,053,832	0	C	A	100.0%	252.8 / NA	71	L64I (CTC→ATC)	shiA	shikimate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (36/35);  total (36/35)

TCGCGCCCGACGAGCTGCGTTAGGCAGCTTCGCTGGTGCCGTCGTCGACTGGTATGATTTTTTACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGCTCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGACCGTCTGGATGATGGGCATCGCGACA  >  NC_000913/2053696‑2053966                                                                                                                                         |                                                                                                                                        tCGCGCCCGACGAGCTGCGTTAGGCAGCTTCGCTGGTGCCGTCGTCGACTGGTATGATTTTTTACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATcg                                                                                                                                      >  1:929853/1‑140 (MQ=255)    cgcCCGACGAGCTGCGTTAGGCAGCTTCGCTGGTGCCGTCGTCGACTGGTATGATTTTTTACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATcgccg                                                                                                                                   <  2:857809/140‑1 (MQ=255)    cgcCCGACGAGCTGCGTTAGGCAGCTTCGCTGGTGCCGTCGTCGACTGGTATGATTTTTTACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATcgccg                                                                                                                                   >  1:575485/1‑140 (MQ=255)     gcCCGACGAGCTGCGTTAGGCAGCTTCGCTGGTGCCGTCGTCGACTGGTATGATTTTTTACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATcgccgc                                                                                                                                  >  1:82993/1‑140 (MQ=255)                         cAGCTTCGCTGGTGCCGTCGTCGACTGGTATGATTTTTTACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑g                                                                                                             <  2:308375/140‑1 (MQ=255)                            cTTCGCTGGTGCCGTCGTCGACTGGTATGATTTTTTACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGt                                                                                                                <  2:301002/135‑1 (MQ=255)                            cTTCGCTGGTGCCGTCGTCGACTGGTATGATTTTTTACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGt                                                                                                                >  1:301002/1‑135 (MQ=255)                            cTTCGCTGGTGCCGTCGTCGACTGGTATGATTTTTTACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGAt                                                                                                          >  1:711369/1‑140 (MQ=255)                            cTTCGCTGGTGCCGTCGTCGACTGGTATGATTTTTTACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGAt                                                                                                          >  1:499729/1‑140 (MQ=255)                                 cTGGTGCCGTCGTCGACTGGTATGATTTTTTACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCtt                                                                                                     <  2:992600/140‑1 (MQ=255)                                          tcgtcgACTGGTATGATTTTTTACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTtccgtccg                                                                                            <  2:718655/140‑1 (MQ=255)                                            gtcgACTGGTATGATTTTTTACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCt                                                                                          <  2:412418/140‑1 (MQ=255)                                                  tGGTATGATTTTTTACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTcggcgg                                                                                    <  2:656932/140‑1 (MQ=255)                                                     tATGATTTTTTACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGgtgt                                                                                 <  2:499729/140‑1 (MQ=255)                                                          ttttttACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCAttt                                                                            >  2:1035495/1‑140 (MQ=255)                                                            ttttACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTc                                                                          >  1:792133/1‑140 (MQ=255)                                                             tttACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCg                                                                         <  2:750552/140‑1 (MQ=255)                                                              ttACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCgg                                                                        >  2:598308/1‑140 (MQ=255)                                                              ttACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCgg                                                                        >  1:506025/1‑140 (MQ=255)                                                              ttACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCgg                                                                        >  2:580541/1‑140 (MQ=255)                                                              ttACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCgg                                                                        >  1:396078/1‑140 (MQ=255)                                                              ttACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCgg                                                                        >  2:1016358/1‑140 (MQ=255)                                                               tACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGgtg                                                                                  >  2:268651/1‑129 (MQ=255)                                                               tACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGgtg                                                                                  <  1:268651/129‑1 (MQ=255)                                                               tACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGt                                                                       >  1:383741/1‑140 (MQ=255)                                                                   ctATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACt                                                                   >  1:463482/1‑140 (MQ=255)                                                                    tATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACtt                                                                  >  2:703531/1‑140 (MQ=255)                                                                     aTGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACttt                                                                 >  1:381198/1‑140 (MQ=255)                                                                      tGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTCGGGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACttt                                                                 >  1:617270/1‑140 (MQ=255)                                                                      tGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCa                                                                               >  1:164154/1‑125 (MQ=255)                                                                      tGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCa                                                                               <  2:164154/125‑1 (MQ=255)                                                                      tGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTg                                                                >  2:368021/1‑140 (MQ=255)                                                                         cATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGcg                                                             >  1:833263/1‑140 (MQ=255)                                                                         cATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGcg                                                             <  2:463482/140‑1 (MQ=255)                                                                         cATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGcg                                                             >  1:643840/1‑140 (MQ=255)                                                                         cATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGcg                                                             >  2:775193/1‑140 (MQ=255)                                                                         cATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGcg                                                             <  2:949319/140‑1 (MQ=255)                                                                              ccgccgCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGcgaccga                                                        >  1:160227/1‑140 (MQ=255)                                                                               cgccgcACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGcgaccgac                                                       <  1:368021/140‑1 (MQ=255)                                                                               cgccgcACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGcgaccgac                                                       <  2:383741/140‑1 (MQ=255)                                                                                gccgcACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACt                                                      >  1:177876/1‑140 (MQ=255)                                                                                  cgcACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACCTTGGCGACCGACTgg                                                    >  2:648062/1‑140 (MQ=255)                                                                                           gtTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTC‑TTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAgcg                                          <  2:929853/140‑1 (MQ=255)                                                                                            tttAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAgcg                                          <  2:82993/140‑1 (MQ=255)                                                                                            tttAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAgcg                                          <  1:384430/140‑1 (MQ=255)                                                                                            tttAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAgcg                                          <  1:356044/140‑1 (MQ=255)                                                                                            tttAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAgcg                                          <  1:333345/140‑1 (MQ=255)                                                                                            tttAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAgcg                                          <  2:792133/140‑1 (MQ=255)                                                                                            tttAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAgcg                                          <  1:703531/140‑1 (MQ=255)                                                                                                aTCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATg                                      <  2:575485/140‑1 (MQ=255)                                                                                                 tCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGt                                     >  1:920155/1‑140 (MQ=255)                                                                                                   gcgAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAgcg                                          >  1:727417/1‑133 (MQ=255)                                                                                                   gcgAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAgcg                                          <  2:727417/133‑1 (MQ=255)                                                                                                   gcgAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTa                                   >  1:636232/1‑140 (MQ=255)                                                                                                   gcgAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTa                                   >  1:723153/1‑140 (MQ=255)                                                                                                   gcgAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGGCCTTTTCGGTCCCTTTTGCGAGCGCCTGGGACGTAAGCGCATGTTa                                   >  2:281254/1‑140 (MQ=255)                                                                                                         ttttCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTg                             <  1:1054934/140‑1 (MQ=255)                                                                                                           ttCCCGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGAc                           <  2:711369/140‑1 (MQ=255)                                                                                                              ccGCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGACCGt                        <  2:160227/140‑1 (MQ=255)                                                                                                                gCAAGTAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGACCGTCt                      <  2:95017/140‑1 (MQ=255)                                                                                                                     tAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCt                              <  1:965700/127‑1 (MQ=255)                                                                                                                     tAAGCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCt                              >  2:965700/1‑127 (MQ=255)                                                                                                                        gCCCGGCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGACCGTCTGGatgatg              <  2:506025/140‑1 (MQ=255)                                                                                                                            ggCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGACCGTCTGGATGATGGGCa          >  2:333099/1‑140 (MQ=255)                                                                                                                            ggCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGACCGTCTGGATGATGGGCa          >  1:876131/1‑140 (MQ=255)                                                                                                                             gCGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGACCGTCTGGATGATGGGCAt         <  2:731555/140‑1 (MQ=255)                                                                                                                              cGATGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGACCGTCTGGATGATGGGCATc        >  2:148169/1‑140 (MQ=255)                                                                                                                                 tGGGAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGACCGTCTGGATGATGGGCATcgcg     <  1:148169/140‑1 (MQ=255)                                                                                                                                  gggAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGACCGTCTGGATGATGGGCATCGCGa    <  2:723153/140‑1 (MQ=255)                                                                                                                                  gggAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGACCGTCTGGATGATGGGCATCGCGa    <  1:1035495/140‑1 (MQ=255)                                                                                                                                    gAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGACCGTCTGGATGATGGGCATCGCGACa  <  2:381198/140‑1 (MQ=255)                                                                                                                                    gAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGACCGTCTGGATGATGGGCATCGCGACa  <  2:833263/140‑1 (MQ=255)                                                                                                                                    gAACGATCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGACCGTCTGGATGATGGGCATCGCGACa  <  1:598308/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TCGCGCCCGACGAGCTGCGTTAGGCAGCTTCGCTGGTGCCGTCGTCGACTGGTATGATTTTTTACTCTATGGCATCACCGCCGCACTGGTGTTTAATCGCGAGTTTTTCCCGCAAGTAAGCCCGGCGATGGGAACGCTCGCCGCATTTGCTACCTTTGGCGTC‑GGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGACCGTCTGGATGATGGGCATCGCGACA  >  NC_000913/2053696‑2053966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑