Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,630,356	G→T	A11S (GCC→TCC)	yfgI →	nalidixic acid resistance protein YfgI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,630,356	0	G	T	100.0%	296.1 / NA	83	A11S (GCC→TCC)	yfgI	nalidixic acid resistance protein YfgI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (45/38);  total (45/38)

GCCAGTTTACGACAGGTTTAGCCGTTGTTATTACCACGACGTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTAGCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGAATGGGTCGCGGAGCGCAACAG  >  NC_000913/2630219‑2630483                                                                                                                                          |                                                                                                                                gCCAGTTTACGACAGGTTTAGCCGTTGTTATTACCACGACGTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATcc                                                                                                                               >  2:559225/1‑140 (MQ=255)  ccAGTTTACGACAGGTTTAGCCGTTGTTATTACCACGACGTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATcct                                                                                                                              >  2:697762/1‑140 (MQ=255)  ccAGTTTACGACAGGTTTAGCCGTTGTCATTACCACGACGTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATcct                                                                                                                              >  1:774640/1‑140 (MQ=255)    aGTTTACGACAGGTTTAGCCGTTGTTATTACCACGACGTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTAtcctcc                                                                                                                            <  1:618850/140‑1 (MQ=255)      tttACGACAGGTTTAGCCGTTGTTATTACCACGACGTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCtt                                                                                                                          >  1:128468/1‑140 (MQ=255)                gTTTAGCCGTTGTTATTACCACGACGTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCg                                                                                                                >  1:753415/1‑140 (MQ=255)                gTTTAGCCGTTGTTATTACCACGACGTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCg                                                                                                                >  2:2407/1‑140 (MQ=255)                gTTTAGCCGTTGTTATTACCACGACGTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCg                                                                                                                >  2:791412/1‑140 (MQ=255)                  ttAGCCGTTGTTATTACCACGACGTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGc                                                                                                              >  1:549740/1‑140 (MQ=255)                        gttgttATTACCACGACGTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGc                                                                                                        <  1:791412/140‑1 (MQ=255)                          tgttATTACCACGACGTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCt                                                                                                      <  2:372361/140‑1 (MQ=255)                          tgttATTACCACGACGTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCt                                                                                                      >  2:785506/1‑140 (MQ=255)                          tgttATTACCACGACGTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCt                                                                                                      >  1:120518/1‑140 (MQ=255)                             tattaCCACGACGTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCtcat                                                                                                   <  2:549740/140‑1 (MQ=255)                                 aCCACGACGTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCt                                                                                                             >  2:669066/1‑126 (MQ=255)                                 aCCACGACGTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCt                                                                                                             <  1:669066/126‑1 (MQ=255)                                 aCCACGACGTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCAtt                                                                                               <  1:2407/140‑1 (MQ=255)                                   cACGACGTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGc                                                                                             >  2:741981/1‑140 (MQ=255)                                      gacgTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCt                                                                                                      <  1:494892/128‑1 (MQ=255)                                      gacgTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCt                                                                                                      >  2:494892/1‑128 (MQ=255)                                      gacgTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAgg                                                                                          >  2:235909/1‑140 (MQ=255)                                      gacgTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAgg                                                                                          >  2:63792/1‑140 (MQ=255)                                      gacgTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAgg                                                                                          >  2:519732/1‑140 (MQ=255)                                       acgTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCtcat                                                                                                   >  2:742157/1‑130 (MQ=255)                                       acgTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCtcat                                                                                                   <  1:742157/130‑1 (MQ=255)                                        cgTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGAt                                                                                        <  2:753415/140‑1 (MQ=255)                                        cgTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGAt                                                                                        <  2:128468/140‑1 (MQ=255)                                        cgTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGAt                                                                                        <  1:697762/140‑1 (MQ=255)                                        cgTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGAt                                                                                        <  2:944372/140‑1 (MQ=255)                                        cgTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGAt                                                                                        <  2:35450/140‑1 (MQ=255)                                        cgTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGAt                                                                                        <  1:559225/140‑1 (MQ=255)                                        cgTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGAt                                                                                        <  1:90633/140‑1 (MQ=255)                                                    cgcgTATTCAGCCAAATACCAAATGTCCTTAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTctgct                                                                            >  2:1043836/1‑140 (MQ=255)                                                    cgcgTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGc                                                                                             >  1:883088/1‑123 (MQ=255)                                                    cgcgTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGc                                                                                             <  2:883088/123‑1 (MQ=255)                                                    cgcgTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTctgct                                                                            >  2:896073/1‑140 (MQ=255)                                                    cgcgTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTctgct                                                                            >  2:942225/1‑140 (MQ=255)                                                    cgcgTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTctgct                                                                            >  2:96438/1‑140 (MQ=255)                                                    cgcgTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTctgct                                                                            >  2:995638/1‑140 (MQ=255)                                                    cgcgTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTctgct                                                                            >  2:1103049/1‑140 (MQ=255)                                                    cgcgTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTctgct                                                                            >  1:325391/1‑140 (MQ=255)                                                      cgTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGAt                                                                                        >  2:695699/1‑126 (MQ=255)                                                      cgTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGAt                                                                                        <  1:695699/126‑1 (MQ=255)                                                           tCAGCCAAATACCAAATGTCCTTAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAg                                                                     <  1:1043836/140‑1 (MQ=255)                                                            cAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGa                                                                    <  1:96438/140‑1 (MQ=255)                                                              gCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAg                                                                  <  2:120518/140‑1 (MQ=255)                                                              gCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAg                                                                  <  2:395183/140‑1 (MQ=255)                                                                  aaTACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGa                                                              >  1:507552/1‑140 (MQ=255)                                                                      ccAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAg                                                          <  1:63792/140‑1 (MQ=255)                                                                      ccAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAg                                                          <  1:785506/140‑1 (MQ=255)                                                                       cAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGc                                                         <  1:519732/140‑1 (MQ=255)                                                                       cAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGc                                                         >  1:794835/1‑140 (MQ=255)                                                                       cAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGc                                                         >  1:909279/1‑140 (MQ=255)                                                                        taaTGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCa                                                        <  1:235909/139‑1 (MQ=255)                                                                         aaTGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCaa                                                       <  2:507552/140‑1 (MQ=255)                                                                              ccTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTaa                                                  >  2:569687/1‑140 (MQ=255)                                                                               cTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTaaa                                                 >  2:440937/1‑140 (MQ=255)                                                                                tGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTaaaa                                                >  2:95827/1‑140 (MQ=255)                                                                                 gAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGC‑AGGTAAAAAt                                              >  2:965025/1‑140 (MQ=255)                                                                                    aaGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGa                                            <  1:741981/140‑1 (MQ=255)                                                                                            ataatCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCaaa                                    <  1:569687/140‑1 (MQ=255)                                                                                                 cAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGgcag                               >  2:221206/1‑140 (MQ=255)                                                                                                  aggaggAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGgcagc                              >  2:202550/1‑140 (MQ=255)                                                                                                   ggaggaGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCaaa                                    >  1:224021/1‑133 (MQ=255)                                                                                                   ggaggaGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCaaa                                    <  2:224021/133‑1 (MQ=255)                                                                                                   ggaggaGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGgcagca                             >  2:38038/1‑140 (MQ=255)                                                                                                    gaggagTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCGAAGGCAGCAt                            <  2:794835/140‑1 (MQ=255)                                                                                                    gaggagTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCAt                            <  1:995638/140‑1 (MQ=255)                                                                                                         gTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAgg                                  <  1:664214/129‑1 (MQ=255)                                                                                                         gTCATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAgg                                  >  2:664214/1‑129 (MQ=255)                                                                                                           cATGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGAAt                     <  1:202550/140‑1 (MQ=255)                                                                                                            aTGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGAATg                    >  2:1104057/1‑140 (MQ=255)                                                                                                            aTGAAGAAAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGAATg                    >  1:1030923/1‑140 (MQ=255)                                                                                                              gaagaaAGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGAATggg                  >  2:283701/1‑140 (MQ=255)                                                                                                                  aaaGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGAATGGGTcgc              >  1:275609/1‑140 (MQ=255)                                                                                                                  aaaGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGAATGGGTcgc              >  2:888425/1‑140 (MQ=255)                                                                                                                   aaGTTTTTCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGAATGGGTcgcg             <  2:1030923/140‑1 (MQ=255)                                                                                                                      tttttCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGAATGGGTCGCGGAg          <  1:440937/140‑1 (MQ=255)                                                                                                                      tttttCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGAATGGGTCGCGGAg          <  1:38038/140‑1 (MQ=255)                                                                                                                      tttttCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGAATGGGTCGCGGAg          <  1:283701/140‑1 (MQ=255)                                                                                                                      tttttCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGAATGGGTCGCGGAg          <  2:940839/140‑1 (MQ=255)                                                                                                                      tttttCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGAATGGGTCGCGGAg          <  1:942225/140‑1 (MQ=255)                                                                                                                      tttttCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGAATGGGTCGCGGAg          <  1:1104057/140‑1 (MQ=255)                                                                                                                        tttCTTTGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGAATGGGTCGCGGAgcg        >  2:1045395/1‑140 (MQ=255)                                                                                                                              tGCGCCATCTTATCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGAATGGGTCGCGGAGCGCAACAg  >  2:369970/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                GCCAGTTTACGACAGGTTTAGCCGTTGTTATTACCACGACGTATAACGAAACGCGTATTCAGCCAAATACCAAATGTCCTGAAAAGAGCTAATAATCAGGAGGAGTCATGAAGAAAGTTTTTCTTTGCGCCATCTTAGCCTCCTTAAGCTATCCGGCTATCGCCTCATCATTGCAGGATCAACTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGAATGGGTCGCGGAGCGCAACAG  >  NC_000913/2630219‑2630483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑