Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,998,998	G→T	noncoding (60/74 nt)	glyU ←	tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,998,998	0	G	T	100.0%	269.4 / NA	79	noncoding (60/74 nt)	glyU	tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (43/36);  total (43/36)

AGATAACCACTTATTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGCTGCTGACTGCTGATTAATTCGCC  >  NC_000913/2998861‑2999134                                                                                                                                          |                                                                                                                                         aGATAACCACTTATTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGa                                                                                                                                        >  2:968704/1‑140 (MQ=255) aGATAACCACTTATTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGa                                                                                                                                        >  2:561702/1‑140 (MQ=255) aGATAACCACTTATTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGa                                                                                                                                        >  2:553796/1‑140 (MQ=255)     aaCCACTTATTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCg                                                                                                                                    <  1:36389/140‑1 (MQ=255)       ccACTTATTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGaa                                                                                                                                  <  2:840377/140‑1 (MQ=255)       ccACTTATTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGaa                                                                                                                                  <  2:1092189/140‑1 (MQ=255)       ccACTTATTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGaa                                                                                                                                  >  2:1088640/1‑140 (MQ=255)       ccACTTATTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGaa                                                                                                                                  <  1:553796/140‑1 (MQ=255)        cACTTATTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGaa                                                                                                                                  >  1:101412/1‑139 (MQ=255)        cACTTATTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGaa                                                                                                                                  >  2:538096/1‑139 (MQ=255)        cACTTATTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGaa                                                                                                                                  <  2:101412/139‑1 (MQ=255)        cACTTATTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGaa                                                                                                                                  <  1:538096/139‑1 (MQ=255)          cTTATTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAAccc                                                                                                                               <  2:423465/140‑1 (MQ=255)          cTTATTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAAccc                                                                                                                               <  1:637442/140‑1 (MQ=255)          cTTATTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAAccc                                                                                                                               <  1:561702/140‑1 (MQ=255)             aTTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGaa                                                                                                                                  >  1:709920/1‑134 (MQ=255)             aTTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGaa                                                                                                                                  <  2:709920/134‑1 (MQ=255)             aTTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCg                                                                                                                            >  1:1005357/1‑140 (MQ=255)             aTTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCg                                                                                                                            >  2:727727/1‑140 (MQ=255)                 aacaacTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGtata                                                                                                                        >  2:316523/1‑140 (MQ=255)                        tGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTg                                                                                                                 >  1:133686/1‑140 (MQ=255)                            aaTAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATag                                                                                                                       >  1:206321/1‑130 (MQ=255)                            aaTAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATag                                                                                                                       <  2:206321/130‑1 (MQ=255)                                   tgtTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCg                                                                                                      >  1:40115/1‑140 (MQ=255)                                    gtTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGt                                                                                                     >  1:260388/1‑140 (MQ=255)                                     ttttACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGct                                                                                                          <  2:646725/134‑1 (MQ=255)                                     ttttACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGct                                                                                                          >  1:646725/1‑134 (MQ=255)                                     ttttACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGtt                                                                                                    <  1:968704/140‑1 (MQ=255)                                          ccTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTAcc                                                                                               >  1:84322/1‑140 (MQ=255)                                            tGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCAt                                                                                             >  1:142218/1‑140 (MQ=255)                                            tGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCAt                                                                                             <  1:159416/140‑1 (MQ=255)                                            tGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCAt                                                                                             <  1:669113/140‑1 (MQ=255)                                               gttgttACAAAAGGATTGCATTGCGTTAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGa                                                                                          >  2:168811/1‑140 (MQ=255)                                               gttgttACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGa                                                                                          >  1:816106/1‑140 (MQ=255)                                               gttgttACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGa                                                                                          >  2:343567/1‑140 (MQ=255)                                                  gttACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACt                                                                                       >  2:1095266/1‑140 (MQ=255)                                                  gttACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACt                                                                                       >  1:53016/1‑140 (MQ=255)                                                   ttACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTa                                                                                      >  1:876739/1‑140 (MQ=255)                                                    tACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTAc                                                                                     >  2:989906/1‑140 (MQ=255)                                                         aaGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCg                                                                                >  2:531832/1‑140 (MQ=255)                                                              ttgcattgcGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCg                                                                           >  1:330251/1‑140 (MQ=255)                                                                gcattgcGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCgag                                                                         <  2:352843/140‑1 (MQ=255)                                                                gcattgcGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCgag                                                                         <  2:816106/140‑1 (MQ=255)                                                                gcattgcGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCgag                                                                         <  2:84322/140‑1 (MQ=255)                                                                gcattgcGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCgag                                                                         <  2:133686/140‑1 (MQ=255)                                                                gcattgcGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCgag                                                                         <  2:142218/140‑1 (MQ=255)                                                                      cGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGGTAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCgagactcga                                                                   >  1:782942/1‑135 (MQ=255)                                                                      cGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGt                                                                   >  1:1026991/1‑140 (MQ=255)                                                                      cGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGt                                                                   >  1:812084/1‑140 (MQ=255)                                                                       gTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTa                                                                  <  2:478637/140‑1 (MQ=255)                                                                       gTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCATa                                                                  <  1:66663/140‑1 (MQ=255)                                                                         aaaCGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCTGGCGAAGTGAATCGAACCCCCGTATAGAGCTTGGGAAGCTCTCGTTTTACCATTGAGCTACGCCTGCTTCGAGATGCGTAAg                                                                <  2:106085/140‑1 (MQ=255)                                                                         aaaCGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAg                                                                <  2:261093/140‑1 (MQ=255)                                                                          aaCGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAgg                                                               <  1:775264/140‑1 (MQ=255)                                                                          aaCGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAgg                                                               <  2:1026991/140‑1 (MQ=255)                                                                                ttttATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATtat                                                         <  1:168811/140‑1 (MQ=255)                                                                                  ttatttaCAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATaa                                                       <  1:727727/140‑1 (MQ=255)                                                                                       taCAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCtt                                                  >  1:859825/1‑140 (MQ=255)                                                                                       taCAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCtt                                                  >  2:1059892/1‑140 (MQ=255)                                                                                        acaacaAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTa                                                 <  1:989906/140‑1 (MQ=255)                                                                                           acaaAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGc                                              <  2:876739/140‑1 (MQ=255)                                                                                           acaaAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGc                                              <  2:260388/140‑1 (MQ=255)                                                                                                   gggAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACg                                               <  1:81096/131‑1 (MQ=255)                                                                                                   gggAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACg                                               >  2:81096/1‑131 (MQ=255)                                                                                                     gAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGc                                    <  1:316523/140‑1 (MQ=255)                                                                                                      aaGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCa                                   >  2:571047/1‑140 (MQ=255)                                                                                                           ttACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGc                              >  1:711582/1‑140 (MQ=255)                                                                                                           ttACGGCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGc                              >  2:395354/1‑140 (MQ=255)                                                                                                               ggCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACg                          >  2:208419/1‑140 (MQ=255)                                                                                                                gCGAAGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACgc                         >  1:988155/1‑140 (MQ=255)                                                                                                                   aaGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACgctgc                      >  1:1046922/1‑140 (MQ=255)                                                                                                                   aaGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACgctgc                      >  2:182608/1‑140 (MQ=255)                                                                                                                   aaGATAAATTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCT‑CCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACgctgct                     >  2:6914/1‑140 (MQ=255)                                                                                                                         aaTTGGAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGctgctgactg                <  2:599605/140‑1 (MQ=255)                                                                                                                              gAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGCTGCTGACTGCTGAt           <  1:531832/140‑1 (MQ=255)                                                                                                                              gAGCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGCTGCTGACTGCTGAt           <  1:1023053/140‑1 (MQ=255)                                                                                                                                gCGGGCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGCTGCTGACTGCTGATTa         >  2:13702/1‑140 (MQ=255)                                                                                                                                   ggCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGCTGCTGACTGCTGATTAAtt      >  2:684308/1‑140 (MQ=255)                                                                                                                                   ggCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGCTGCTGACTGCTGATTAAtt      >  1:353450/1‑140 (MQ=255)                                                                                                                                   ggCGAAGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGCTGCTGACTGCTGATTAAtt      >  2:247015/1‑140 (MQ=255)                                                                                                                                       aaGTGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGCTGCTGACTGCTGATTAATTCGcc  <  1:247015/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         AGATAACCACTTATTTAACAACTTGAAAATAAGGTGTTTTACCTGGGTTGTTACAAAAGGATTGCATTGCGTAAACGCTTTTTATTTACAACAAAATGGGGAAGTATTACGGCGAAGATAAATTGGAGCGGGCGAAGGGAATCGAACCCTCGTATAGAGCTTGGGAAGCTCTCGTTCTACCATTGAACTACGCCCGCTTCGAGATGCGTAAGGCATTATAAACCTTACGCTCTCCTTAGCAAGTGCCACGCTGCTGACTGCTGATTAATTCGCC  >  NC_000913/2998861‑2999134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑