Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,309,282	C→A	V629V (GTG→GTT)	pnp ←	polynucleotide phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,309,282	0	C	A	100.0%	204.5 / NA	58	V629V (GTG→GTT)	pnp	polynucleotide phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (29/29);  total (29/29)

CGGTACTTCCTGACCCATCTGCAGGTAATCGGTCACTTTCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTCACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGCAATCTTCACAGTACCGTCATCTTCGATTTCGATGGTGGTG  >  NC_000913/3309150‑3309417                                                                                                                                     |                                                                                                                                        cGGTACTTCCTGACCCATCTGCAGGTAATCGGTCACTTTCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTAc                                                                                                                                  <  2:330283/140‑1 (MQ=255) cGGTACTTCCTGACCCATCTGCAGGTAATCGGTCACTTTCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTAc                                                                                                                                  <  1:1099819/140‑1 (MQ=255)  ggTACTTCCTGACCCATCTGCAGGTAATCGGTCACTTTCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTAcc                                                                                                                                 >  1:250075/1‑140 (MQ=255)      cTTCCTGACCCATCTGCAGGTAATCGGTCACTTTCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAgtg                                                                                                                             <  1:696877/140‑1 (MQ=255)                 aTCTGCAGGTAATCGGTCACTTTCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGcc                                                                                                                  <  2:250075/140‑1 (MQ=255)                  tCTGCAGGTAATCGGTCACTTTCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGccc                                                                                                                 >  1:93726/1‑140 (MQ=255)                         gTAATCGGTCACTTTCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGa                                                                                                          >  2:452204/1‑140 (MQ=255)                          tAATCGGTCACTTTCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGAt                                                                                                         <  1:452204/140‑1 (MQ=255)                            aTCGGTCACTTTCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGAttt                                                                                                       >  2:270942/1‑140 (MQ=255)                            aTCGGTCACTTTCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGAttt                                                                                                       >  1:152989/1‑140 (MQ=255)                             tCGGTCACTTTCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTc                                                                                                      >  2:1106693/1‑140 (MQ=255)                              cGGTCACTTTCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCt                                                                                                     <  1:345590/140‑1 (MQ=255)                                gTCACTTTCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTc                                                                                                            <  1:963991/131‑1 (MQ=255)                                gTCACTTTCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTc                                                                                                            >  2:963991/1‑131 (MQ=255)                                gTCACTTTCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGc                                                                                                   >  1:444112/1‑140 (MQ=255)                                 tCACTTTCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATACGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCa                                                                                                  <  1:595/140‑1 (MQ=255)                                     tttCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGa                                                                                              >  1:549910/1‑140 (MQ=255)                                      ttCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGAt                                                                                             <  2:46758/140‑1 (MQ=255)                                      ttCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGAt                                                                                             <  2:408934/140‑1 (MQ=255)                                      ttCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGAt                                                                                             <  1:297204/140‑1 (MQ=255)                                           aaCGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCtt                                                                                        >  2:953449/1‑140 (MQ=255)                                             cgcgTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCg                                                                                      >  1:807983/1‑140 (MQ=255)                                                 tttGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATac                                                                                  >  2:400775/1‑140 (MQ=255)                                                 tttGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATac                                                                                  >  1:739964/1‑140 (MQ=255)                                                  ttGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATacg                                                                                 <  1:270942/140‑1 (MQ=255)                                                   tGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATacga                                                                                >  1:363619/1‑140 (MQ=255)                                                         cGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATacgac                                                                               <  1:172898/135‑1 (MQ=255)                                                         cGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATacgac                                                                               >  2:172898/1‑135 (MQ=255)                                                                 gagaTGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGttt                                                                  <  1:879034/140‑1 (MQ=255)                                                                          aCCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTtctc                                                         >  1:72163/1‑140 (MQ=255)                                                                           ccAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCg                                                        <  1:1106693/140‑1 (MQ=255)                                                                               aCCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgt                                                    >  1:493896/1‑140 (MQ=255)                                                                               aCCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgt                                                    >  2:348076/1‑140 (MQ=255)                                                                                   tcttTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggt                                                <  2:272616/140‑1 (MQ=255)                                                                                    cttTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtc                                               >  2:492058/1‑140 (MQ=255)                                                                                    cttTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtc                                               >  2:315133/1‑140 (MQ=255)                                                                                     tttACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtcg                                              <  1:492058/140‑1 (MQ=255)                                                                                        aCCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggt                                                >  2:75742/1‑135 (MQ=255)                                                                                        aCCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggt                                                <  1:75742/135‑1 (MQ=255)                                                                                         ccgccgccgATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGc                                          >  2:596556/1‑140 (MQ=255)                                                                                          cgccgccgATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGCa                                         <  1:98216/140‑1 (MQ=255)                                                                                          cgccgccgATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGCa                                         <  2:152989/140‑1 (MQ=255)                                                                                           gccgccgATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGCaa                                        >  2:719993/1‑140 (MQ=255)                                                                                            ccgccgATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGCAAt                                       <  2:363619/140‑1 (MQ=255)                                                                                             cgccgATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGCAATc                                      >  2:868027/1‑140 (MQ=255)                                                                                              gccgATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGCAATCt                                     >  1:552165/1‑140 (MQ=255)                                                                                              gccgATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGCAATCt                                     <  2:739964/140‑1 (MQ=255)                                                                                               ccgATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGCAATCtt                                    <  1:719993/140‑1 (MQ=255)                                                                                               ccgATGGCAACAAATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGCAATCtt                                    <  2:549910/140‑1 (MQ=255)                                                                                                         caaATGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGCAATCTTCACAGTACCg                          <  1:868027/140‑1 (MQ=255)                                                                                                            aTGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGCAATCTTCACAGTACCg                          >  2:148484/1‑137 (MQ=255)                                                                                                            aTGCGCCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGCAATCTTCACAGTACCg                          <  1:148484/137‑1 (MQ=255)                                                                                                               cgcCAAAGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGCAATCTTCACAGTACCGTCATCt                    >  1:148155/1‑140 (MQ=255)                                                                                                                    aaGTCAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGCAATCTTCACAGTACCGTCATCttcgat               <  2:1044158/140‑1 (MQ=255)                                                                                                                        cAACGATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGCAATCTTCACAGTACCGTCATCttcgatttcg           <  2:552165/140‑1 (MQ=255)                                                                                                                            gATACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGCAATCTTCACAGTACCGTCATCTTCGATTTCGAtgg       <  2:807983/140‑1 (MQ=255)                                                                                                                              tACGGGTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGCAATCTTCACAGTACCGTCATCTTCGATTTCGAtggtg     >  1:1138385/1‑140 (MQ=255)                                                                                                                                 gggTAACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGCAATCTTCACAGTACCGTCATCTTCGATTTCGATGgtggtg  >  1:614273/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        CGGTACTTCCTGACCCATCTGCAGGTAATCGGTCACTTTCTCAACGCGTTTGTCAGCGATTTGAGAGATGTGGACCAGACCTTCTTTACCGCCGCCGATGGCAACAAATGCGCCAAAGTCAACGATACGGGTCACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCGCTGCAATCTTCACAGTACCGTCATCTTCGATTTCGATGGTGGTG  >  NC_000913/3309150‑3309417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑