Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,453,657	C→A	E91* (GAA→TAA)	gspB ←	putative general secretion pathway protein B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,453,657	0	C	A	100.0%	297.8 / NA	84	E91* (GAA→TAA)	gspB	putative general secretion pathway protein B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (38/46);  total (38/46)

CGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATACCCACCAA  >  NC_000913/3453522‑3453788                                                                                                                                        |                                                                                                                                    cgttcAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTagacg                                                                                                                                 >  2:780376/1‑140 (MQ=255) cgttcAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTagacg                                                                                                                                 >  2:1085863/1‑140 (MQ=255) cgttcAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTagacg                                                                                                                                 >  2:626136/1‑140 (MQ=255)   ttcAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAgacgac                                                                                                                               >  2:457921/1‑140 (MQ=255)   ttcAGTGCATTTTTGACACGCCCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAgacgac                                                                                                                               >  1:530822/1‑140 (MQ=255)      aGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACggg                                                                                                                            >  1:39559/1‑140 (MQ=255)       gTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACgggg                                                                                                                           <  1:166177/140‑1 (MQ=255)        tGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGa                                                                                                                          >  1:918312/1‑140 (MQ=255)        tGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGa                                                                                                                          >  2:471132/1‑140 (MQ=255)         gCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAg                                                                                                                         <  1:132071/140‑1 (MQ=255)             ttttGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGc                                                                                                                     >  2:698516/1‑140 (MQ=255)                     cGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGtttttt                                                                                                             <  1:941250/140‑1 (MQ=255)                       ctctctTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTga                                                                                                           <  2:376637/140‑1 (MQ=255)                       ctctctTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTga                                                                                                           <  1:856290/140‑1 (MQ=255)                       ctctctTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTga                                                                                                           <  1:631254/140‑1 (MQ=255)                       ctctctTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTga                                                                                                           <  2:919710/140‑1 (MQ=255)                            ttAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGaaa                                                                                                      <  1:863125/140‑1 (MQ=255)                            ttAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGaaa                                                                                                      <  1:698516/140‑1 (MQ=255)                            ttAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGaaa                                                                                                      <  2:490733/140‑1 (MQ=255)                              aaaCCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATa                                                                                                    <  2:442872/140‑1 (MQ=255)                              aaaCCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATa                                                                                                    <  2:343960/140‑1 (MQ=255)                              aaaCCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATa                                                                                                    <  1:1085863/140‑1 (MQ=255)                                 ccGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAgtg                                                                                                 >  2:295977/1‑140 (MQ=255)                                  cGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGaaa                                                                                                      >  2:953698/1‑134 (MQ=255)                                  cGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGaaa                                                                                                      <  1:261039/134‑1 (MQ=255)                                  cGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGaaa                                                                                                      >  2:261039/1‑134 (MQ=255)                                  cGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGaaa                                                                                                      <  1:953698/134‑1 (MQ=255)                                    gCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTgaag                                                                                                         <  2:169016/129‑1 (MQ=255)                                    gCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTgaag                                                                                                         >  1:169016/1‑129 (MQ=255)                                    gCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAgtgttg                                                                                              >  2:654527/1‑140 (MQ=255)                                    gCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAgtgttg                                                                                              >  1:607856/1‑140 (MQ=255)                                    gCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAgtgttg                                                                                              >  1:691839/1‑140 (MQ=255)                                    gCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAgtgttg                                                                                              >  2:254363/1‑140 (MQ=255)                                    gCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAgt                                                                                                  <  1:549526/136‑1 (MQ=255)                                    gCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAgt                                                                                                  >  2:549526/1‑136 (MQ=255)                                          tctTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGa                                                                                        <  2:956816/140‑1 (MQ=255)                                          tctTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGa                                                                                        <  1:471132/140‑1 (MQ=255)                                          tctTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGa                                                                                        <  1:783137/140‑1 (MQ=255)                                           ctTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGAt                                                                                       <  1:744785/140‑1 (MQ=255)                                               gCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGa                                                                                        <  2:565909/135‑1 (MQ=255)                                               gCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGa                                                                                        >  1:565909/1‑135 (MQ=255)                                                   tcttcCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTaaa                                                                               >  1:368711/1‑140 (MQ=255)                                                   tcttcCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTaaa                                                                               >  2:215005/1‑140 (MQ=255)                                                    cttcCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTaaaa                                                                              >  2:128632/1‑140 (MQ=255)                                                           aGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCg                                                                       <  1:457921/140‑1 (MQ=255)                                                            gTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGt                                                                      >  2:150692/1‑140 (MQ=255)                                                             tATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGtt                                                                     <  1:954544/140‑1 (MQ=255)                                                             tATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGtt                                                                     >  1:686866/1‑140 (MQ=255)                                                             tATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGtt                                                                     <  2:691839/140‑1 (MQ=255)                                                                  tcttcGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGc                                                                <  2:660931/140‑1 (MQ=255)                                                                    ttcGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCct                                                              <  1:274407/140‑1 (MQ=255)                                                                    ttcGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCct                                                              >  2:527588/1‑140 (MQ=255)                                                                     tcGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTc                                                                   >  1:792551/1‑134 (MQ=255)                                                                     tcGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTc                                                                   <  2:792551/134‑1 (MQ=255)                                                                      cGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCctct                                                            <  1:527588/140‑1 (MQ=255)                                                                       gTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGCTTCAGCCTCTa                                                           >  1:562405/1‑140 (MQ=255)                                                                         cTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACa                                                         >  1:180548/1‑140 (MQ=255)                                                                               aagaagaGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACt                                                   <  1:558143/140‑1 (MQ=255)                                                                               aagaagaGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACt                                                   <  2:519185/140‑1 (MQ=255)                                                                               aagaagaGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACt                                                   <  1:1112442/140‑1 (MQ=255)                                                                                agaagaGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACtt                                                  <  2:180548/140‑1 (MQ=255)                                                                                agaagaGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACtc                                                  <  1:150692/140‑2 (MQ=255)                                                                                      gggggCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCtt                                            >  1:317538/1‑140 (MQ=255)                                                                                       ggggCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCttt                                           >  1:473882/1‑140 (MQ=255)                                                                                       ggggCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCttt                                           >  2:614651/1‑140 (MQ=255)                                                                                         ggCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTa                                         >  2:823111/1‑140 (MQ=255)                                                                                         ggCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTa                                         <  2:1050412/140‑1 (MQ=255)                                                                                               ctcAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATcc                                   <  1:713238/140‑1 (MQ=255)                                                                                                 cAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCaa                                 <  2:317538/140‑1 (MQ=255)                                                                                                  aaCCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCaaa                                <  2:473882/140‑1 (MQ=255)                                                                                                  aaCCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCaaa                                <  2:363901/140‑1 (MQ=255)                                                                                                  aaCCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCaaa                                <  2:918312/140‑1 (MQ=255)                                                                                                       cgACGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCAcc                           >  1:124273/1‑140 (MQ=255)                                                                                                         aCGCCTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCa                         >  1:1004141/1‑140 (MQ=255)                                                                                                            ccTGGGTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAg                      <  1:780376/140‑1 (MQ=255)                                                                                                               gggTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTg                   >  1:474056/1‑140 (MQ=255)                                                                                                                ggTCATCTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGc                  <  2:1004141/140‑1 (MQ=255)                                                                                                                      cTTCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCa            <  1:614651/140‑1 (MQ=255)                                                                                                                       ttCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCAt           >  2:236264/1‑140 (MQ=255)                                                                                                                       ttCCTCCTCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCAt           <  1:295977/140‑1 (MQ=255)                                                                                                                         cctcctCGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATAc         >  1:898455/1‑140 (MQ=255)                                                                                                                            cctcGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATAcccc      >  2:924320/1‑139 (MQ=255)                                                                                                                            cctcGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATACcca      <  2:562405/140‑1 (MQ=255)                                                                                                                            cctcGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATACcca      <  2:39559/140‑1 (MQ=255)                                                                                                                             ctcGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATACccac     >  1:668922/1‑140 (MQ=255)                                                                                                                               cGACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCTAAAGCACCCATAGCTGCCTGGCATACccacca   >  2:1088112/1‑140 (MQ=255)                                                                                                                                gACGGATTAGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATACCCaccaa  >  1:1025734/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    CGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATACCCACCAA  >  NC_000913/3453522‑3453788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑