Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	622,465	C→A	L56I (CTT→ATT)	entS →	enterobactin exporter EntS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	622,465	0	C	A	100.0%	265.8 / NA	74	L56I (CTT→ATT)	entS	enterobactin exporter EntS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (35/39);  total (35/39)

GCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCTTCATTGGACTGTGCCT  >  NC_000913/622331‑622601                                                                                                                                       |                                                                                                                                         gCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCg                                                                                                                                     >  1:74764/1‑140 (MQ=255)    tGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTg                                                                                                                                  >  1:753907/1‑140 (MQ=255)     gTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGa                                                                                                                                 <  1:533971/140‑1 (MQ=255)     gTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGa                                                                                                                                 >  1:89971/1‑140 (MQ=255)      ttGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGAc                                                                                                                                >  2:903033/1‑140 (MQ=255)      ttGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGAc                                                                                                                                >  2:1130358/1‑140 (MQ=255)      ttGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGAc                                                                                                                                >  1:670707/1‑140 (MQ=255)         aaaaCGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCt                                                                                                                             <  2:860464/140‑1 (MQ=255)            aCGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGAc                                                                                                                          <  2:753907/140‑1 (MQ=255)            aCGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGAc                                                                                                                          >  2:538813/1‑140 (MQ=255)                aCCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACcggc                                                                                                                      <  1:42706/140‑1 (MQ=255)                  ccGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACcggcgg                                                                                                                    >  1:276006/1‑140 (MQ=255)                   cGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCggcggc                                                                                                                   >  2:904445/1‑140 (MQ=255)                    ggCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGgcggcg                                                                                                                  <  1:1130358/140‑1 (MQ=255)                    ggCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGgcggcg                                                                                                                  <  1:538813/140‑1 (MQ=255)                      cGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGcgcg                                                                                                                <  1:692561/140‑1 (MQ=255)                          tCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGAtgt                                                                                                            >  1:1104006/1‑140 (MQ=255)                          tCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGAtgt                                                                                                            >  1:579832/1‑140 (MQ=255)                              gcAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGAtgtttgt                                                                                                        <  2:1108986/140‑1 (MQ=255)                               cAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGAtgtttgtt                                                                                                       <  1:445233/140‑1 (MQ=255)                                aGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGTGATTTCGGTGACGCTGACCGGCGGCGCGATGTttgttg                                                                                                      <  2:889776/140‑1 (MQ=255)                                   aTTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGcc                                                                                                   <  1:453/140‑1 (MQ=255)                                   aTTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGcc                                                                                                   <  2:870398/140‑1 (MQ=255)                                     tCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTg                                                                                                 <  1:38896/140‑1 (MQ=255)                                          gctcgTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGt                                                                                            <  2:1113887/140‑1 (MQ=255)                                          gctcgTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGt                                                                                            <  2:276006/140‑1 (MQ=255)                                            tcgTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGAtgtttgt                                                                                                        >  1:235427/1‑126 (MQ=255)                                            tcgTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGAtgtttgt                                                                                                        <  2:235427/126‑1 (MQ=255)                                            tcgTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTcg                                                                                          >  1:417525/1‑140 (MQ=255)                                              gTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTcggc                                                                                        >  2:549742/1‑140 (MQ=255)                                              gTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTcggc                                                                                        >  2:599331/1‑140 (MQ=255)                                               ttccATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTcggcg                                                                                       <  2:435835/137‑1 (MQ=255)                                                ttCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTcggcgg                                                                                      <  2:579832/140‑1 (MQ=255)                                                        aaTTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTgg                                                                              >  1:206992/1‑140 (MQ=255)                                                         aTTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGc                                                                             >  1:474915/1‑140 (MQ=255)                                                          ttGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCg                                                                            >  2:1119854/1‑140 (MQ=255)                                                           tgtgTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACAGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCgg                                                                           <  1:104844/140‑1 (MQ=255)                                                               tctcTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCg                                                                       >  2:8287/1‑140 (MQ=255)                                                                       tttGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAgc                                                               >  1:618614/1‑140 (MQ=255)                                                                           cTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCaa                                                           <  1:903033/140‑1 (MQ=255)                                                                             cGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCaaaa                                                         <  2:670707/140‑1 (MQ=255)                                                                               gCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCaaaaaa                                                       >  1:33191/1‑140 (MQ=255)                                                                                cGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAg                                                      >  1:566342/1‑140 (MQ=255)                                                                                     cggtgccggtgcAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGAtt                                                 >  1:204900/1‑140 (MQ=255)                                                                                            ggtgcAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTgg                                          <  2:474453/140‑1 (MQ=255)                                                                                              tgcAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGgcg                                        <  2:190217/140‑1 (MQ=255)                                                                                                  gATCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGcgcgcg                                    >  2:632435/1‑140 (MQ=255)                                                                                                   aaccAGATGATGACACATTCCACCTGGCAGGTCGGGATTTCGGTGACGCGGACCGGCGGCGCGATGTTTGTTCGCCTGATGGTGGGCGGTGTGCTGGCGGATCGATATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCgg                                   <  2:1092684/138‑1 (MQ=255)                                                                                                   aTCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCgg                                   <  1:848065/140‑1 (MQ=255)                                                                                                   aTCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCgg                                   <  2:204900/140‑1 (MQ=255)                                                                                                   aTCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCgg                                   <  1:890490/140‑1 (MQ=255)                                                                                                   aTCCAGATGATGACACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCgg                                   <  2:1049426/140‑1 (MQ=255)                                                                                                             tgaCACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGc                         >  2:717290/1‑140 (MQ=255)                                                                                                             tgaCACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGc                         >  1:436076/1‑140 (MQ=255)                                                                                                             tgaCACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGc                         >  2:431002/1‑140 (MQ=255)                                                                                                              gaCACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCt                              <  1:787862/134‑1 (MQ=255)                                                                                                              gaCACATTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCt                              >  2:787862/1‑134 (MQ=255)                                                                                                                 acaTTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTg                     >  2:660036/1‑140 (MQ=255)                                                                                                                  caTTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTgg                    <  2:236995/140‑1 (MQ=255)                                                                                                                  caTTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTgg                    <  1:290933/140‑1 (MQ=255)                                                                                                                  caTTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTgg                    <  2:206992/140‑1 (MQ=255)                                                                                                                  caTTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTgg                    <  2:89971/140‑1 (MQ=255)                                                                                                                  caTTCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTgg                    <  1:861488/140‑1 (MQ=255)                                                                                                                     tCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCtt                 >  1:477236/1‑140 (MQ=255)                                                                                                                     tCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCtt                 >  2:659964/1‑140 (MQ=255)                                                                                                                     tCCACCTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCtt                 >  2:498651/1‑140 (MQ=255)                                                                                                                         ccTGGCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCTTCAtt             >  1:58926/1‑140 (MQ=255)                                                                                                                            ggCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCTTCATTGGa          <  1:1118351/140‑1 (MQ=255)                                                                                                                             gCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTgg                    <  2:24464/129‑1 (MQ=255)                                                                                                                             gCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTgg                    >  1:24464/1‑129 (MQ=255)                                                                                                                             gCAGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCTTCATTGGAc         >  1:165811/1‑140 (MQ=255)                                                                                                                               aGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCTTCATTGGACtg       <  1:659964/140‑1 (MQ=255)                                                                                                                               aGGTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCTTCATTGGACtg       <  2:165811/140‑1 (MQ=255)                                                                                                                                 gTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCTTCATTGGACtgtg     <  2:618614/140‑1 (MQ=255)                                                                                                                                 gTGGGGATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCTTCATTGGACtgtg     <  1:387484/140‑1 (MQ=255)                                                                                                                                    gggATTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCTTCATTGGACTGTGCCt  <  1:549742/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         GCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCTTCATTGGACTGTGCCT  >  NC_000913/622331‑622601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑