| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,604,784 | G→T | R131L (CGC→CTC) | rsmD → | 16S rRNA m(2)G966 methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,604,784 | 0 | G | T | 98.4% | 213.3 / ‑3.3 | 61 | R131L (CGC→CTC) | rsmD | 16S rRNA m(2)G966 methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (29/31); total (30/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CGCGGTTTCTCAGCAGTTAATTAAGAATCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCGCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGA > NC_000913/3604647‑3604919 | cgcgGTTTCTCAGCAGTTAATTAAGAATCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTtcctcc > 2:833428/1‑140 (MQ=255)cgcgGTTTCTCAGCAGTTAATTAAGAATCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTtcctcc > 2:1106572/1‑140 (MQ=255)cgcgGTTTCTCAGCAGTTAATTAAGAATCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTtcctcc > 2:280508/1‑140 (MQ=255) gTTTCTCAGCAGTTAATTAAGAATCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTgg < 2:1063588/140‑1 (MQ=255) gTTTCTCAGCAGTTAATTAAGAATCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTgg < 2:1094641/140‑1 (MQ=255) gTTTCTCAGCAGTTAATTAAGAATCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTgg < 2:235695/140‑1 (MQ=255) gTTTCTCAGCAGTTAATTAAGAATCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTgg < 1:433589/140‑1 (MQ=255) gTTTCTCAGCAGTTAATTAAGAATCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTga < 1:780185/140‑2 (MQ=255) tttCTCAGCAGTTAATTAAGAATCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGc > 2:326957/1‑140 (MQ=255) tttCTCAGCAGTTAATTAAGAATCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGc < 2:449137/140‑1 (MQ=255) ttCTCAGCAGTTAATTAAGAATCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCt < 1:95905/140‑1 (MQ=255) ttCTCAGCAGTTAATTAAGAATCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCt < 1:684890/140‑1 (MQ=255) tcAGCAGTTAATTAAGAATCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGt < 1:75539/140‑1 (MQ=255) gTTAATTAAGAATCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTagaag > 1:370755/1‑140 (MQ=255) aaGAATCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGAt < 1:1060312/140‑1 (MQ=255) aGAATCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTagaa > 2:1012104/1‑131 (MQ=255) aGAATCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTagaa < 1:1012104/131‑1 (MQ=255) aTCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTTAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAAt > 1:848376/1‑140 (MQ=255) aTCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAAt > 1:869326/1‑140 (MQ=255) tCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAAtt < 2:370755/140‑1 (MQ=255) cTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAAttt > 1:450336/1‑140 (MQ=255) tGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTa > 2:700649/1‑140 (MQ=255) ggCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTAc < 1:280508/140‑1 (MQ=255) gACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTgg < 2:869326/140‑1 (MQ=255) gACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTgg < 2:450336/140‑1 (MQ=255) tAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGAt < 1:833428/140‑1 (MQ=255) ggCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTgg > 2:424183/1‑127 (MQ=255) ggCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTgg < 1:424183/127‑1 (MQ=255) ggCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGAt < 1:182593/132‑1 (MQ=255) ggCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGAt > 2:182593/1‑132 (MQ=255) ggCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACggctg > 1:771127/1‑140 (MQ=255) gcgTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGAc > 2:152313/1‑140 (MQ=255) cgTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACg > 1:207423/1‑140 (MQ=255) gtgAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGc < 1:102694/140‑1 (MQ=255) gtgAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGc > 1:662238/1‑140 (MQ=255) tgAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGcc < 1:1106572/140‑1 (MQ=255) aaCAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCt > 1:315132/1‑140 (MQ=255) cgcgATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATg > 2:901284/1‑140 (MQ=255) cgATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTc > 1:749585/1‑140 (MQ=255) aTGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCGCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGa > 2:484875/1‑140 (MQ=255) tCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAg > 1:643945/1‑140 (MQ=255) aTTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCg < 1:853185/140‑1 (MQ=255) ggCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCg > 1:599319/1‑140 (MQ=255) aaaaaGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAAc < 2:207423/140‑1 (MQ=255) aaaaGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACg < 2:749585/140‑1 (MQ=255) acacCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGcc < 2:848376/140‑1 (MQ=255) acCGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCa < 1:152313/140‑1 (MQ=255) cGCATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACt > 2:500856/1‑140 (MQ=255) cATAATATCGTGTTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGt > 2:69048/1‑140 (MQ=255) tgtTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCaaa < 2:319910/139‑1 (MQ=255) tgtTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCaaa > 1:319910/1‑139 (MQ=255) tgtTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAAc < 1:987783/140‑1 (MQ=255) gtTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACt > 2:380172/1‑140 (MQ=255) gtTTGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACt < 1:700649/140‑1 (MQ=255) tGTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGt > 1:621172/1‑140 (MQ=255) gTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTc > 1:497385/1‑140 (MQ=255) gTCGATCCACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTc < 1:500856/140‑1 (MQ=255) cACCGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACAt < 1:326957/140‑1 (MQ=255) cGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCa > 1:1107445/1‑122 (MQ=255) cGTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCa < 2:1107445/122‑1 (MQ=255) gTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCggg > 1:398029/1‑140 (MQ=255) gTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCggg > 2:167993/1‑140 (MQ=255) gTTCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCggg > 2:855752/1‑140 (MQ=255) ttCCTCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGa > 2:94727/1‑140 (MQ=255) | CGCGGTTTCTCAGCAGTTAATTAAGAATCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAACAGCAACGCGATGTCATTCCTGGCGCAAAAAGGTACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCGCCGTGGCTTGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGA > NC_000913/3604647‑3604919 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |