Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,926,265	T→C	D64G (GAC→GGC)	mioC ←	flavoprotein MioC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,926,265	0	T	C	100.0%	155.4 / NA	44	D64G (GAC→GGC)	mioC	flavoprotein MioC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/22);  total (22/22)

GTTCTGCCTCGAGTTTATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTG  >  NC_000913/3926131‑3926400                                                                                                                                       |                                                                                                                                        gTTCTGCCTCGAGTTTATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGa                                                                                                                                    <  2:675018/140‑1 (MQ=255)    cTGCCTCGAGTTTATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAata                                                                                                                                 >  1:528986/1‑140 (MQ=255)          cGAGTTTATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCg                                                                                                                           <  1:161226/140‑1 (MQ=255)               ttATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCAcc                                                                                                                      <  1:1059735/140‑1 (MQ=255)                 aTCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCgt                                                                                                                    >  1:361823/1‑140 (MQ=255)                      tAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCgtgggtg                                                                                                               >  1:602897/1‑140 (MQ=255)                       aGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCgtgggtgg                                                                                                              >  1:134050/1‑140 (MQ=255)                       aGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCACCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCgtgggtgg                                                                                                              >  2:336145/1‑140 (MQ=255)                            cacaAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTg                                                                                                         <  1:887435/140‑1 (MQ=255)                                   ggTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAAcca                                                                                                  >  1:561541/1‑140 (MQ=255)                                    gtgtCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAAccag                                                                                                 >  2:444824/1‑140 (MQ=255)                                       tCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAAccagcca                                                                                              <  1:1124771/140‑1 (MQ=255)                                       tCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAAccagcca                                                                                              <  1:287973/140‑1 (MQ=255)                                        cATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAAccagccag                                                                                             <  2:528986/140‑1 (MQ=255)                                        cATATTCACGACTGCCAATACCGATTGCACCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAAccagccag                                                                                             <  1:336145/140‑1 (MQ=255)                                         atatTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGa                                                                                            >  1:811014/1‑140 (MQ=255)                                                cGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGa                                                                                     <  2:940017/140‑1 (MQ=255)                                                 gACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAg                                                                                    >  1:1041511/1‑140 (MQ=255)                                                  aCTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAgg                                                                                   <  1:1034144/140‑1 (MQ=255)                                                  aCTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAgg                                                                                   <  2:361823/140‑1 (MQ=255)                                                      ccAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAgg                                                                               >  2:289409/1‑140 (MQ=255)                                                        aaTACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTa                                                                             <  2:853655/140‑1 (MQ=255)                                                        aaTACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTa                                                                             <  1:137556/140‑1 (MQ=255)                                                          tACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTaaa                                                                           <  2:835450/140‑1 (MQ=255)                                                              gATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAAtctt                                                                       >  1:99412/1‑140 (MQ=255)                                                               aTTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAAtcttc                                                                      <  2:561541/140‑1 (MQ=255)                                                                ttGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAAtcttct                                                                     >  1:921099/1‑140 (MQ=255)                                                                 tGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTa                                                                    >  1:474862/1‑140 (MQ=255)                                                                     ccAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAg                                                                >  1:756288/1‑140 (MQ=255)                                                                              aCTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTg                                                       <  2:474862/140‑1 (MQ=255)                                                                                        gATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCg                                             <  2:811014/140‑1 (MQ=255)                                                                                          tCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCggg                                           >  2:661639/1‑139 (MQ=255)                                                                                            gggCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCggtgg                                         >  2:247527/1‑140 (MQ=255)                                                                                            gggCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCggtgg                                         >  1:111026/1‑140 (MQ=255)                                                                                                   tGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAAcc                                  >  2:1075214/1‑140 (MQ=255)                                                                                                           cAATGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGGAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGCGGTAAAACCCGCCTCtt                          >  2:645031/1‑140 (MQ=255)                                                                                                             aTGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTcc                        <  1:661639/140‑1 (MQ=255)                                                                                                             aTGCTTCATAGAAAGGAGAAAGGTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTcc                        <  2:1041511/140‑1 (MQ=255)                                                                                                                     tAGAAAGGAGAAAGGTTGCCCGGAATATCTCCCGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAAACCGCCTCTTCCAGCTTTTc                >  2:432977/1‑140 (MQ=255)                                                                                                                              gaaaggTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGCCCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGgtg       <  1:479021/140‑1 (MQ=255)                                                                                                                              aaaaggTTGCCCGGAATACCGCCGGCACGGTGGGTGGAGCTGAAAACCACCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGCCCGTGCAGCGTTTCGGTGGAAAACCCCGCCTCTTCCAGCTTTTCAGCCAGgtg       <  2:99412/139‑1 (MQ=255)                                                                                                                               aaaggTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAgg         >  1:811465/1‑137 (MQ=255)                                                                                                                               aaaggTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAgg         <  2:811465/137‑1 (MQ=255)                                                                                                                                aaggTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGACCCCTGAGGCAGGTAAATCTTCTAACAGCGGCCCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGtt     <  2:2393/140‑1 (MQ=255)                                                                                                                                  ggTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCTGCCTCTTCCAGCTTTTCAGCCAGGTGTTCt   <  1:444824/140‑1 (MQ=255)                                                                                                                                  ggTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGATAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGGGTTCt   >  2:811615/1‑140 (MQ=255)                                                                                                                                   gTTGCCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATACCCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCTTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTg  <  2:602897/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        GTTCTGCCTCGAGTTTATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTG  >  NC_000913/3926131‑3926400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑