Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,149,878	C→A	A130S (GCC→TCC)	eptC ←	phosphoethanolamine transferase EptC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,149,878	0	C	A	100.0%	253.6 / NA	71	A130S (GCC→TCC)	eptC	phosphoethanolamine transferase EptC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (42/29);  total (42/29)

ACGTATTCATGGCGATCGGATGCAGAATCAAGCCATAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGCCAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGACGTAGTAGCACAGCG  >  NC_000913/4149749‑4150012                                                                                                                                  |                                                                                                                                       aCGTATTCATGGCGATCGGATGCAGAATCAAGCCATAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATa                                                                                                                              >  1:612636/1‑140 (MQ=255)      ttCATGGCGATCGGATGCAGAATCAAGCCATAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGcac                                                                                                                         >  1:484877/1‑140 (MQ=255)      ttCATGGCGATCGGATGCAGAATCAAGCCATAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGcac                                                                                                                         <  2:291018/140‑1 (MQ=255)      ttCATGGCGATCGGATGCAGAATCAAGCCATAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGcac                                                                                                                         >  1:151676/1‑140 (MQ=255)      ttCATGGCGATCGGATGCAGAATCAAGCCATAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGcac                                                                                                                         >  1:614325/1‑140 (MQ=255)      ttCATGGCGATCGGATGCAGAATCAAGCCATAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGcac                                                                                                                         >  1:716806/1‑140 (MQ=255)           ggggagcGGATGCAGCATAAAGCCATAAAGCAGGGCAAAAGAGAGAACAGAACGCCACGGATGGGGAAAATAGACCGGGCGGAGGCAAGTCCACAGCACAACTGCCACAACAAGACAGGGCAGCGCGATAAGCACAAttt                                                                                                                    >  2:194399/7‑140 (MQ=255)           ggCGATCGGATGCAGAATCAAGCCATAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAAttt                                                                                                                    >  1:448521/1‑140 (MQ=255)           ggCGATCGGATGCAGAATCAAGCCATAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAAttt                                                                                                                    >  1:27229/1‑140 (MQ=255)            gCGATCGGATGCAGAATCAAGCCATAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAAtttt                                                                                                                   >  1:1117389/1‑140 (MQ=255)            gCGATCGGATGCAGAATCAAGCCATAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAAtttt                                                                                                                   >  2:1026090/1‑140 (MQ=255)            gCGATCGGATGCAGAATCAAGCCATAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAAtttt                                                                                                                   >  1:246714/1‑140 (MQ=255)             cGATAGGATGCAGAATCAAGCCATAAAGCAGCGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACCGCGCGATAAGCACAATTTTc                                                                                                                  >  2:830443/1‑140 (MQ=255)               aTCGGATGCAGAATCAAGCCATAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAg                                                                                                                >  2:1002472/1‑140 (MQ=255)                         gaacaaaGCAATAAAGCAGGGCAAAGGAGACAACAAAACGCCACGGCTTGGGAATATAGCCCGGGCGCGGGCGGGTCCACAGCAGAACTGCAACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATAc                                                                                                      <  2:91156/135‑1 (MQ=255)                          aaTCAAGCCATAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACt                                                                                                     <  2:1117389/140‑1 (MQ=255)                          aaTCAAGCCATAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACt                                                                                                     >  2:665232/1‑140 (MQ=255)                          aaTCAAGCCATAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACt                                                                                                     <  1:1026090/140‑1 (MQ=255)                           aTCAAGCCATAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGCCCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTg                                                                                                    <  2:176294/140‑1 (MQ=255)                               aGCCATAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCtt                                                                                                >  1:91156/1‑140 (MQ=255)                                    tAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGc                                                                                                  >  2:201334/1‑133 (MQ=255)                                    tAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGc                                                                                                  <  1:201334/133‑1 (MQ=255)                                    tAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATa                                                                                           >  2:651000/1‑140 (MQ=255)                                          aGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCt                                                                                     >  2:867749/1‑140 (MQ=255)                                            ggCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTgg                                                                                   >  2:206903/1‑140 (MQ=255)                                            ggCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTgg                                                                                   >  2:151728/1‑140 (MQ=255)                                            ggCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTgg                                                                                   >  2:562077/1‑140 (MQ=255)                                            ggCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTgg                                                                                   >  2:1002348/1‑140 (MQ=255)                                                 aaGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCg                                                                              <  2:448521/140‑1 (MQ=255)                                                      acaacaTAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCgttggt                                                                         >  2:482473/1‑140 (MQ=255)                                                           aTAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCgttggtgttgg                                                                    >  1:1099616/1‑140 (MQ=255)                                                           aTAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCgttggtgttgg                                                                    >  2:263735/1‑140 (MQ=255)                                                             aaCGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGtt                                                                  <  1:400400/140‑1 (MQ=255)                                                                 ccACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGa                                                              <  1:562077/140‑1 (MQ=255)                                                                 ccACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGa                                                              <  1:867749/140‑1 (MQ=255)                                                                 ccACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGa                                                              <  1:1002472/140‑1 (MQ=255)                                                                 ccACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGa                                                              <  1:187282/140‑1 (MQ=255)                                                                  cACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGaa                                                             >  2:421194/1‑140 (MQ=255)                                                                    cGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACa                                                           >  1:378098/1‑140 (MQ=255)                                                                    cGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACa                                                           >  1:270862/1‑140 (MQ=255)                                                                     ggCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACAt                                                          >  2:1009555/1‑140 (MQ=255)                                                                     ggCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACAt                                                          <  2:1099616/140‑1 (MQ=255)                                                                     ggCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACAt                                                          >  2:614632/1‑140 (MQ=255)                                                                     ggCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACAt                                                          <  1:126426/140‑1 (MQ=255)                                                                      gCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATc                                                         <  1:151728/140‑1 (MQ=255)                                                                        tttGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATcac                                                       <  1:263735/140‑1 (MQ=255)                                                                              atatAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAg                                                 <  2:452941/140‑1 (MQ=255)                                                                                   gACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGc                                            <  1:482473/140‑1 (MQ=255)                                                                                    aCCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGct                                           >  2:819568/1‑140 (MQ=255)                                                                                      cGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGctct                                         >  1:400771/1‑140 (MQ=255)                                                                                       gggCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTg                                        >  1:106788/1‑140 (MQ=255)                                                                                         gcgcAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCg                                      >  2:60763/1‑140 (MQ=255)                                                                                          cgcAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCga                                     <  2:716806/140‑1 (MQ=255)                                                                                          cgcAGGCGTGTCCACAGAAGAACTCCCACCTCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCga                                     <  1:830443/140‑1 (MQ=255)                                                                                          atagggCGTTTCCACAGAAAAACCCCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCga                                     <  2:291992/136‑1 (MQ=255)                                                                                                  tgtCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCt                             <  2:483696/140‑1 (MQ=255)                                                                                                   gtCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTg                            >  1:700796/1‑140 (MQ=255)                                                                                                    tCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGc                                            <  1:142824/123‑1 (MQ=255)                                                                                                    tCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGc                                            >  2:142824/1‑123 (MQ=255)                                                                                                    tCCACAGCAGAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGa                           >  2:808089/1‑140 (MQ=255)                                                                                                         agcagAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGAcctgt                      >  1:291992/1‑137 (MQ=255)                                                                                                         agcagAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTa                      >  1:695826/1‑140 (MQ=255)                                                                                                            agAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTAGAt                   <  1:206903/140‑1 (MQ=255)                                                                                                             gAACTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAATGCTCTGCGAGAACTCCTGACCGTAGATg                  >  2:749991/1‑140 (MQ=255)                                                                                                              aaCTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGa                 >  2:439083/1‑140 (MQ=255)                                                                                                              aaCTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGa                 <  1:819568/140‑1 (MQ=255)                                                                                                              aaCTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGa                 >  1:785970/1‑140 (MQ=255)                                                                                                              aaCTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGa                 <  1:665232/140‑1 (MQ=255)                                                                                                              aaCTGCCACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGa                 >  1:633398/1‑140 (MQ=255)                                                                                                                   ccACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGACgtag            <  2:484877/140‑1 (MQ=255)                                                                                                                   ccACCGCCGTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGACgtag            <  2:612636/140‑1 (MQ=255)                                                                                                                           gTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGACGTAGTAGCACAg    <  1:194399/140‑1 (MQ=255)                                                                                                                           gTATAGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGACGTAGTAGCACAg    <  1:1009555/140‑1 (MQ=255)                                                                                                                             ataGGACAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGACGTAGTAGCACAgcg  >  2:72927/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                       ACGTATTCATGGCGATCGGATGCAGAATCAAGCCATAAAGCAGGGCAAAAGAGACAACATAACGCCACGGCTTTGGAATATAGACCGGGCGCAGGCGTGTCCACAGCAGAACTGCCACCGCCGTATAGGCCAGCGCGATAAGCACAATTTTCAGGCTGAAATACTGGCTTAAATACTCGCTGGCTTCGTTGGTGTTGGTTTCGAACATCACAAACAGAACGCTCTGCGAGAACTCCTGACCGTAGATGACGTAGTAGCACAGCG  >  NC_000913/4149749‑4150012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑