Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,189,902	G→T	E39D (GAG→GAT)	yjaZ →	conserved protein YjaZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,189,902	0	G	T	100.0%	266.0 / NA	75	E39D (GAG→GAT)	yjaZ	conserved protein YjaZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/48);  total (27/48)

ACTTTCTTAAGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGAGCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCAC  >  NC_000913/4189767‑4190034                                                                                                                                        |                                                                                                                                     aCTTTCTTAAGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCtt                                                                                                                                  >  2:1134597/1‑140 (MQ=255)        tAAGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTa                                                                                                                           <  2:1054674/140‑1 (MQ=255)         aaGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTAt                                                                                                                          <  1:213784/140‑1 (MQ=255)         aaGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTAt                                                                                                                          <  1:809064/140‑1 (MQ=255)               cGAGAATGAAGCAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAGGATCCTTTAGAGTATCGCttt                                                                                                                    >  1:854466/1‑140 (MQ=255)               cGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCttt                                                                                                                    >  1:1041800/1‑140 (MQ=255)               cGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCttt                                                                                                                    >  1:1053809/1‑140 (MQ=255)               cGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCttt                                                                                                                    <  1:1134597/140‑1 (MQ=255)               cGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCttt                                                                                                                    >  1:185575/1‑140 (MQ=255)               cGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCttt                                                                                                                    >  1:88342/1‑140 (MQ=255)               cGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCttt                                                                                                                    >  1:538528/1‑140 (MQ=255)                 agaATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCtt                                                                                                                                  <  2:979712/124‑1 (MQ=255)                 agaATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCtt                                                                                                                                  >  1:979712/1‑124 (MQ=255)                  gaATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTgg                                                                                                                 <  2:992856/140‑1 (MQ=255)                  gaATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTgg                                                                                                                 <  2:689655/140‑1 (MQ=255)                  gaATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTgg                                                                                                                 <  2:619410/140‑1 (MQ=255)                       aaaCAGGAAGTGGAAAAGTGGCGACCTTTTGGCCATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTAc                                                                                                            <  2:914689/140‑1 (MQ=255)                       aaaCAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTAc                                                                                                            >  1:115174/1‑140 (MQ=255)                       aaaCAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTAc                                                                                                            <  1:920736/140‑1 (MQ=255)                       aaaCAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTAc                                                                                                            <  2:645328/140‑1 (MQ=255)                       aaaCAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTAc                                                                                                            <  1:1048160/140‑1 (MQ=255)                          cAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAGGATCCTTTAGAGTATCGCTTTTGGGTTACCTa                                                                                                         <  2:854466/140‑1 (MQ=255)                          cAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTa                                                                                                         <  2:115174/140‑1 (MQ=255)                            ggAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTAct                                                                                                       <  2:538528/140‑1 (MQ=255)                            ggAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTAct                                                                                                       <  2:770462/140‑1 (MQ=255)                            ggAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTAct                                                                                                       <  2:714040/140‑1 (MQ=255)                            ggAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTAct                                                                                                       <  2:1041800/140‑1 (MQ=255)                            ggAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTAct                                                                                                       <  1:314720/140‑1 (MQ=255)                             gAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCt                                                                                                          >  1:699176/1‑136 (MQ=255)                             gAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCt                                                                                                          <  2:699176/136‑1 (MQ=255)                                gTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTActctct                                                                                                   >  1:328597/1‑140 (MQ=255)                                      aaGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTg                                                                                             <  2:621934/140‑1 (MQ=255)                                      aaGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTg                                                                                             <  2:988114/140‑1 (MQ=255)                                       aGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTAc                                                                                                            <  1:564436/124‑1 (MQ=255)                                       aGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTAc                                                                                                            >  2:564436/1‑124 (MQ=255)                                         tGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCtt                                                                                          <  2:606999/140‑1 (MQ=255)                                          ggCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTc                                                                                         <  2:328597/140‑1 (MQ=255)                                             gACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTcaca                                                                                      <  1:476648/140‑1 (MQ=255)                                               ccTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACaaa                                                                                    <  2:638490/140‑1 (MQ=255)                                                cTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACaaaa                                                                                   <  1:393336/140‑1 (MQ=255)                                                cTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACaaaa                                                                                   <  1:627540/140‑1 (MQ=255)                                                 ttttGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAg                                                                                  <  2:496415/140‑1 (MQ=255)                                                  tttGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGa                                                                                 <  1:450472/140‑1 (MQ=255)                                                          tCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAAc                                                                         >  2:871835/1‑140 (MQ=255)                                                          tCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAAc                                                                         >  2:562470/1‑140 (MQ=255)                                                           ccGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTcaca                                                                                      <  2:810424/126‑1 (MQ=255)                                                           ccGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTcaca                                                                                      >  1:810424/1‑126 (MQ=255)                                                               aTGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAg                                                                    <  2:88342/140‑1 (MQ=255)                                                                    gATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGacgaa                                                               <  2:185575/140‑1 (MQ=255)                                                                    gATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGacgaa                                                               <  1:173327/140‑1 (MQ=255)                                                                     atatTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATg                                                                            <  1:100910/126‑1 (MQ=255)                                                                     atatTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATg                                                                            >  2:100910/1‑126 (MQ=255)                                                                     atatTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGacgaac                                                              >  1:909712/1‑140 (MQ=255)                                                                        ttCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGAcgaacgaa                                                           >  2:67250/1‑140 (MQ=255)                                                                         tCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACgaag                                                          >  2:35410/1‑140 (MQ=255)                                                                            tGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGGTTATGAACATCAGACGAACGAAGaaa                                                       >  2:80/1‑140 (MQ=255)                                                                                ttATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCCCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACa                                                   <  1:116048/140‑1 (MQ=255)                                                                                  aTCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAAt                                                 <  1:871835/140‑1 (MQ=255)                                                                                  aTCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAAt                                                 <  1:67250/140‑1 (MQ=255)                                                                                   tCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATc                                                >  1:696402/1‑140 (MQ=255)                                                                                           tGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGt                                        >  1:289708/1‑140 (MQ=255)                                                                                             aTGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACa                                                   <  1:614393/127‑1 (MQ=255)                                                                                             aTGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACa                                                   >  2:614393/1‑127 (MQ=255)                                                                                                 tcatcaGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACa                                                   <  2:438493/123‑1 (MQ=255)                                                                                                 tcatcaGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACa                                                   >  1:438493/1‑123 (MQ=255)                                                                                                 tcatcaGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCAcg                                  <  2:909712/140‑1 (MQ=255)                                                                                                   atcaGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCAcgcg                                >  2:678959/1‑140 (MQ=255)                                                                                                   atcaGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCAcgcg                                >  2:246017/1‑140 (MQ=255)                                                                                                    tcaGGCTGTCTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACgcgc                               <  1:35410/140‑1 (MQ=255)                                                                                                             cTACGTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAAt                      <  2:289708/140‑1 (MQ=255)                                                                                                                 gTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGGTTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCg                  <  1:80/140‑1 (MQ=255)                                                                                                                 gTTCAGCATCATGAGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCg                  <  2:476684/140‑1 (MQ=255)                                                                                                                              aGGGCAAAGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAg     <  2:1053809/140‑1 (MQ=255)                                                                                                                                   aaaGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCAc  >  2:1070310/1‑138 (MQ=255)                                                                                                                                   aaaGATCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCAc  <  1:1070310/138‑1 (MQ=255)                                                                                                                                     agatCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGc    >  1:600211/1‑134 (MQ=255)                                                                                                                                     agatCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGc    <  2:600211/134‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     ACTTTCTTAAGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGAGCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCAC  >  NC_000913/4189767‑4190034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑