Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 766,899 C→T L306L (CTG→TTG)  mngA → 2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913766,8990CT100.0% 293.3 / NA 84L306L (CTG→TTG) mngA2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/49);  total (35/49)

CCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCC  >  NC_000913/766762‑767030
                                                                                                                                         |                                                                                                                                   
ccGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGCGAAAAGTGTCAAAACGGAGTTg                                                                                                                                   >  2:611054/1‑140 (MQ=255)
  gcgcTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGaa                                                                                                                                 <  1:264085/140‑1 (MQ=255)
    gcTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAAc                                                                                                                               <  1:627107/140‑1 (MQ=255)
    gcTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAAc                                                                                                                               <  2:419478/140‑1 (MQ=255)
    gcTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAAc                                                                                                                               <  1:461753/140‑1 (MQ=255)
     cTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACa                                                                                                                              >  2:759431/1‑140 (MQ=255)
       ttCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAgg                                                                                                                            <  2:816466/140‑1 (MQ=255)
         cAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCa                                                                                                                          <  2:307785/140‑1 (MQ=255)
            tGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTg                                                                                                                       <  1:759431/140‑1 (MQ=255)
              ccTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGtt                                                                                                                     >  2:606238/1‑140 (MQ=255)
                tGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGa                                                                                                                   >  1:57550/1‑140 (MQ=255)
                tGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGa                                                                                                                   >  1:492008/1‑140 (MQ=255)
                  ttGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGc                                                                                                                 >  1:569948/1‑140 (MQ=255)
                   tGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCg                                                                                                                <  1:102448/140‑1 (MQ=255)
                    gccgagccgaTTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCgg                                                                                                               >  2:562874/1‑140 (MQ=255)
                      cgagccgaTTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGaa                                                                                                             >  1:1130941/1‑140 (MQ=255)
                       gagccgaTTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAAt                                                                                                            <  2:1130941/140‑1 (MQ=255)
                        agccgaTTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAAtc                                                                                                           >  2:182305/1‑140 (MQ=255)
                          ccgaTTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAAtctc                                                                                                         <  2:57550/140‑1 (MQ=255)
                               tCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTg                                                                                                    <  2:589870/140‑1 (MQ=255)
                               tCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTg                                                                                                    <  2:889624/140‑1 (MQ=255)
                                   cATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGt                                                                                                <  1:311725/140‑1 (MQ=255)
                                    aTGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTa                                                                                               <  2:510395/140‑1 (MQ=255)
                                           aGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGa                                                                                        <  1:873174/140‑1 (MQ=255)
                                            gCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTa                                                                                               <  2:557592/132‑1 (MQ=255)
                                            gCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTa                                                                                               >  1:557592/1‑132 (MQ=255)
                                            gCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTAcc                                                                                             <  1:450566/134‑1 (MQ=255)
                                            gCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTAcc                                                                                             >  2:450566/1‑134 (MQ=255)
                                               ttGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGGTACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGt                                                                                    >  1:77720/1‑140 (MQ=255)
                                                tGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTc                                                                                   <  2:467421/140‑1 (MQ=255)
                                                 gATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTcg                                                                                  >  1:828428/1‑140 (MQ=255)
                                                   tCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATATGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTcgcg                                                                                <  1:1034681/140‑1 (MQ=255)
                                                       acaaGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTC‑TTTGCCGTACCGTTGATTGTCGCgggggg                                                                           >  1:107294/1‑140 (MQ=255)
                                                        caaGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCgggggg                                                                           >  2:1085933/1‑140 (MQ=255)
                                                        caaGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCgggggg                                                                           <  1:562874/140‑1 (MQ=255)
                                                            cgcTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACg                                                                       <  2:569948/140‑1 (MQ=255)
                                                            cgcTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACg                                                                       >  2:347402/1‑140 (MQ=255)
                                                            cgcTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACg                                                                       >  2:380756/1‑140 (MQ=255)
                                                             gcTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACgg                                                                      <  2:828839/140‑1 (MQ=255)
                                                              cTTACCCTCAAGCGT‑GCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTg                                                                    >  1:60320/1‑140 (MQ=255)
                                                                 aCCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCt                                                                  >  2:118320/1‑140 (MQ=255)
                                                                   ccTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTgg                                                                >  2:351884/1‑140 (MQ=255)
                                                                       aaGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGt                                                            <  1:804203/140‑1 (MQ=255)
                                                                       aaGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGt                                                            <  1:606238/140‑1 (MQ=255)
                                                                            tAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCa                                                                         <  1:454851/122‑1 (MQ=255)
                                                                            tAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCa                                                                         >  2:454851/1‑122 (MQ=255)
                                                                             aGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCggg                                                      >  1:733645/1‑139 (MQ=255)
                                                                             aGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGt                                                      >  1:59063/1‑140 (MQ=255)
                                                                                              cgtacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCa                                          <  1:630072/135‑1 (MQ=255)
                                                                                              cgtacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCa                                          >  2:630072/1‑135 (MQ=255)
                                                                                              cgtacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCt                                     >  2:326728/1‑140 (MQ=255)
                                                                                              cgtacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCt                                     >  1:1100457/1‑140 (MQ=255)
                                                                                               gtacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCtt                                    <  2:1022151/140‑1 (MQ=255)
                                                                                               gtacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCtt                                    <  1:948466/140‑1 (MQ=255)
                                                                                               gtacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCtt                                    <  2:59063/140‑1 (MQ=255)
                                                                                               gtacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTC‑TTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTc                                   <  2:107294/140‑1 (MQ=255)
                                                                                                tacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTc                                   >  2:684887/1‑140 (MQ=255)
                                                                                                tacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGGATTACTGTCGCAAATCTTc                                   >  1:895144/1‑140 (MQ=255)
                                                                                                 acAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCg                                  <  1:351884/140‑1 (MQ=255)
                                                                                                 acAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCg                                  <  1:326728/140‑1 (MQ=255)
                                                                                                 acAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCg                                  <  1:1085933/140‑1 (MQ=255)
                                                                                                    gCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGc                               <  2:959728/140‑1 (MQ=255)
                                                                                                    gCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGc                               <  1:347402/140‑1 (MQ=255)
                                                                                                     cAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCt                              <  1:182305/140‑1 (MQ=255)
                                                                                                     cAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCt                              <  1:971514/140‑1 (MQ=255)
                                                                                                          tACGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAg                         >  1:410768/1‑140 (MQ=255)
                                                                                                           aCGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGa                        <  2:1132573/140‑1 (MQ=255)
                                                                                                            cGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGAt                       <  2:492008/140‑1 (MQ=255)
                                                                                                            cGCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGAt                       <  2:77720/140‑1 (MQ=255)
                                                                                                             gCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTTGCGGGATTAATGTCGCAAATCTTCGGGCTACAAGATc                      >  2:370480/1‑140 (MQ=255)
                                                                                                             gCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGAt                       >  1:690104/1‑139 (MQ=255)
                                                                                                             gCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGAt                       <  2:690104/139‑1 (MQ=255)
                                                                                                             gCAACCGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCCTCGGGCTACAAGATc                      >  1:686192/1‑140 (MQ=255)
                                                                                                                  cGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGttt                 >  1:161297/1‑140 (MQ=255)
                                                                                                                  cGGTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGttt                 >  2:8064/1‑140 (MQ=255)
                                                                                                                    gTGAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGACGCGGGATTTCTGTCGCAAAATTTCGGGGCACAAGATCTGgttta               >  2:223156/1‑138 (MQ=255)
                                                                                                                      gAAAAGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATg             <  1:370480/140‑1 (MQ=255)
                                                                                                                        aaaGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATgaa           <  2:686192/140‑1 (MQ=255)
                                                                                                                        aaaGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATgaa           <  1:380756/140‑1 (MQ=255)
                                                                                                                          aGTGTCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATgaaga         <  2:161297/140‑1 (MQ=255)
                                                                                                                            tgtCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGaaa       <  1:325668/140‑1 (MQ=255)
                                                                                                                              tCAAAACGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCCCGGGATTACTGTCGCAAATCTTCGGGATACAAGATCTGTTTAATGAAGaaaaa     >  2:653159/1‑139 (MQ=255)
                                                                                                                                aaaaCGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTc   <  1:611054/140‑1 (MQ=255)
                                                                                                                                aaaaCGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTc   <  2:828428/140‑1 (MQ=255)
                                                                                                                                 aaaCGGAGTTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTcc  <  1:223156/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                   
CCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCC  >  NC_000913/766762‑767030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑