Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,294,541	C→A	pseudogene (782/897 nt)	insZ ←	putative transposase InsZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,294,541	0	C	A	100.0%	182.4 / NA	51	pseudogene (782/897 nt)	insZ	putative transposase InsZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/23);  total (28/23)

CCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACAGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACGCAGGCAGTAGCCAGTGGCGGTTACAACCCTTCTGGTTCAGCGTC  >  NC_000913/1294407‑1294669                                                                                                                                       |                                                                                                                                 cccAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACAGGATAACGGGTTTTTGATATCTTAACCGTCCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCagaaga                                                                                                                             <  1:382866/140‑1 (MQ=255)        gggCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACAGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCa                                                                                                                      <  1:778180/140‑1 (MQ=255)                    tCACTTAAGAGGAGCGGCACTATGTTTCACAGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGa                                                                                                          <  2:328391/140‑1 (MQ=255)                     cACTTAAGAGGAGCGGCACTATGTTTCACAGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGAt                                                                                                         <  2:84028/140‑1 (MQ=255)                     agcTTAAGAGGAGCGGCACTATGTTTCACAGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGAt                                                                                                         <  2:1004684/138‑1 (MQ=255)                           agagGAGCGGCACTATGTTTCACAGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTccc                                                                                                   <  1:311519/140‑1 (MQ=255)                                agCGGCACTATGTTTCACAGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTg                                                                                              >  2:545063/1‑140 (MQ=255)                                agCGGCACTATGTTTCACAGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTg                                                                                              >  1:741889/1‑140 (MQ=255)                                  cGGCACTATGTTTCACAGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTccc                                                                                                   >  1:1036294/1‑133 (MQ=255)                                  cGGCACTATGTTTCACAGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTccc                                                                                                   <  2:1036294/133‑1 (MQ=255)                                  cGGCACTATGTTTCACAGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGAc                                                                                            >  2:812610/1‑140 (MQ=255)                                  cGGCACTATGTTTCACAGGATAACGGGTTTTTGATATCTTAAAAAAAAAAAAAAAAAAAAGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTgct                                                                                            >  1:1004684/1‑138 (MQ=255)                                   ggCACTATGTTTCACAGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACg                                                                                           <  1:715411/140‑1 (MQ=255)                                        tATGTTTCACAGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCgtg                                                                                      <  2:19109/140‑1 (MQ=255)                                        tATGTTTCACAGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCgtg                                                                                      <  2:534241/140‑1 (MQ=255)                                               cacaGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCa                                                                               >  2:928976/1‑140 (MQ=255)                                               cacaGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCa                                                                               >  1:190097/1‑140 (MQ=255)                                               cacaGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCa                                                                               >  1:513760/1‑140 (MQ=255)                                               cacaGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCa                                                                               >  2:358143/1‑140 (MQ=255)                                               cacaGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCa                                                                               >  1:270071/1‑140 (MQ=255)                                                 caGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGc                                                                             <  1:20397/140‑1 (MQ=255)                                                  aGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGct                                                                            <  2:513760/140‑1 (MQ=255)                                                    gATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGctct                                                                          >  1:51469/1‑140 (MQ=255)                                                      tAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCgtg                                                                                      >  1:757489/1‑126 (MQ=255)                                                      tAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCgtg                                                                                      <  2:757489/126‑1 (MQ=255)                                                      tAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGc                                                                             <  2:370100/135‑1 (MQ=255)                                                      tAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGc                                                                             >  1:370100/1‑135 (MQ=255)                                                      tAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTAt                                                                        <  2:51469/140‑1 (MQ=255)                                                        aCGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTcc                                                                                 >  1:512284/1‑129 (MQ=255)                                                        aCGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTcc                                                                                 <  2:512284/129‑1 (MQ=255)                                                         cGGGTTTTTGATATCTTAACCGACCTCGCCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCAt                                                                     >  2:341095/1‑140 (MQ=255)                                                                ttGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGAt                                                              >  1:849398/1‑140 (MQ=255)                                                                      tCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGata                                                        <  2:741889/140‑1 (MQ=255)                                                                       cTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGatat                                                       >  1:17814/1‑140 (MQ=255)                                                                               aCCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCAc                                               >  2:215021/1‑140 (MQ=255)                                                                               aCCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCAc                                               >  2:652869/1‑140 (MQ=255)                                                                                ccTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACg                                              >  2:1056087/1‑140 (MQ=255)                                                                                 cTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACgc                                             >  1:231473/1‑140 (MQ=255)                                                                                 cTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACgc                                             >  2:134111/1‑140 (MQ=255)                                                                                          gATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACGCAGGCAGTAg                                    >  2:601591/1‑140 (MQ=255)                                                                                                 gggggCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACGCAGGCAGTAGCCAGTgg                             <  1:652869/140‑1 (MQ=255)                                                                                                  ggggCGTTTTGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACGCAGGCAGTAGCCAGTGGc                            >  1:43210/1‑140 (MQ=255)                                                                                                        ttttGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACGCAGGCAGTAGCCAGTGGCGGTTAc                      <  1:601591/140‑1 (MQ=255)                                                                                                        ttttGTTATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACGCAGGCAGTAGCCAGTGGCGGTTAc                      <  1:928976/140‑1 (MQ=255)                                                                                                             ttATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACGCAGGCAGTAGcc                                  <  1:840801/123‑1 (MQ=255)                                                                                                             ttATGAACACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACGCAGGCAGTAGcc                                  >  2:840801/1‑123 (MQ=255)                                                                                                                  aaCACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACGCAGGCAGTAGCCAGTGGCGGTTTCAAACCctctt            >  1:27532/1‑139 (MQ=255)                                                                                                                  aaCACCACTTCCAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACGCAGGCAGTAGCCAGTGGCGGTGACGCACCTTCtc            >  2:528013/1‑139 (MQ=255)                                                                                                                            ccAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACGCAGGCAGTAGCCAGTGGCGGTTTCAACCCCTCTTGTTTTGCGtg  >  2:886839/1‑139 (MQ=255)                                                                                                                            ccAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACGCAGGCAGTAGCCAGTGGCGGTTACAACCCTTCTGGTTCAGCGTc  <  2:43210/140‑1 (MQ=255)                                                                                                                            ccAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACGCAGGCAGTAGCCAGTGGCGGTTACAACCCTTCTGGTTCAGCGTc  <  1:134111/140‑1 (MQ=255)                                                                                                                            ccAGAGCACCCAGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACGCAGGCAGTAGCCAGTGGCGGTTACAACCCTTCTGGTTCAGCGTc  <  1:358143/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                 CCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACAGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACGCAGGCAGTAGCCAGTGGCGGTTACAACCCTTCTGGTTCAGCGTC  >  NC_000913/1294407‑1294669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑