Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,367,100	C→A	S271S (TCG→TCT)	pspF ←	DNA‑binding transcriptional dual regulator PspF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,367,100	0	C	A	100.0%	250.4 / NA	71	S271S (TCG→TCT)	pspF	DNA‑binding transcriptional dual regulator PspF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (33/38);  total (33/38)

GAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGA  >  NC_000913/1366965‑1367235                                                                                                                                        |                                                                                                                                        gAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAgg                                                                                                                                     <  1:677226/140‑1 (MQ=255) gAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAgg                                                                                                                                     <  2:497213/140‑1 (MQ=255) gAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAgg                                                                                                                                     <  2:981982/140‑1 (MQ=255)       atgatAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGttt                                                                                                                               >  1:579382/1‑140 (MQ=255)            aaGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGaa                                                                                                                          >  1:37387/1‑140 (MQ=255)             aGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGaaa                                                                                                                         <  1:217637/140‑1 (MQ=255)              gTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCGGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAAc                                                                                                                        >  2:709227/1‑140 (MQ=255)                 aaCCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGc                                                                                                                     >  2:735976/1‑140 (MQ=255)                     ccAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATa                                                                                                                 <  2:579382/140‑1 (MQ=255)                     ccAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATa                                                                                                                 <  1:479330/140‑1 (MQ=255)                       aGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGc                                                                                                               <  2:192561/140‑1 (MQ=255)                         tAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGt                                                                                                             >  2:734957/1‑140 (MQ=255)                         tAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGt                                                                                                             >  2:471611/1‑140 (MQ=255)                         tAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGt                                                                                                             >  1:283206/1‑140 (MQ=255)                         tAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGt                                                                                                             >  1:757449/1‑140 (MQ=255)                         tAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGt                                                                                                             >  2:946057/1‑140 (MQ=255)                                 ccGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGc                                                                                                     >  1:289053/1‑140 (MQ=255)                                    cgcgcTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGa                                                                                                  >  2:364268/1‑140 (MQ=255)                                     gcgcTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGAc                                                                                                 <  2:37387/140‑1 (MQ=255)                                     gcgcTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGAc                                                                                                 <  1:735976/140‑1 (MQ=255)                                     gcgcTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGAc                                                                                                 >  2:284970/1‑140 (MQ=255)                                             ggTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTa                                                                                         <  2:283206/140‑1 (MQ=255)                                               ttATATTTCCCTTGTTGCAAACTGAGTTGCGGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTaaa                                                                                       <  1:709227/140‑1 (MQ=255)                                               ttATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTaaa                                                                                       <  1:471611/140‑1 (MQ=255)                                               ttATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTaaa                                                                                       <  1:364268/140‑1 (MQ=255)                                               ttATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTaaa                                                                                       <  1:946057/140‑1 (MQ=255)                                                  tatTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAggg                                                                                    <  2:609084/140‑1 (MQ=255)                                                   atTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGa                                                                                   >  2:897720/1‑140 (MQ=255)                                                    tttCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGAt                                                                                  <  2:974590/140‑1 (MQ=255)                                                    tttCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGAt                                                                                  <  1:846277/140‑1 (MQ=255)                                                     ttCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATc                                                                                 <  1:664875/140‑1 (MQ=255)                                                       cccTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCaa                                                                               >  2:630575/1‑140 (MQ=255)                                                        ccTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACg                                                                                                <  2:280160/122‑1 (MQ=255)                                                        ccTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACg                                                                                                >  1:280160/1‑122 (MQ=255)                                                        ccTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCaat                                                                              >  1:555724/1‑140 (MQ=255)                                                        ccTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCaat                                                                              >  2:988615/1‑140 (MQ=255)                                                            gttgCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGAACaataact                                                                          >  2:775059/1‑138 (MQ=255)                                                             ttgCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAatga                                                                         >  2:781554/1‑140 (MQ=255)                                                                  aaCTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGtca                                                                    <  2:555724/140‑1 (MQ=255)                                                                       aGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAg                                                               <  1:988615/140‑1 (MQ=255)                                                                       aGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAg                                                               >  1:998435/1‑140 (MQ=255)                                                                       aGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAg                                                               >  1:665669/1‑140 (MQ=255)                                                                       aGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAg                                                               >  1:218589/1‑140 (MQ=255)                                                                          tGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCgg                                                            <  2:998435/140‑1 (MQ=255)                                                                          tGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCgg                                                            <  2:218589/140‑1 (MQ=255)                                                                             agcaACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATa                                                         <  1:150258/140‑1 (MQ=255)                                                                              gcaACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATaa                                                        >  1:659713/1‑140 (MQ=255)                                                                                 aCTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCg                                                     >  2:292573/1‑140 (MQ=255)                                                                                        ttCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGc                                              <  2:289053/140‑1 (MQ=255)                                                                                        ttCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGc                                              <  2:66278/140‑1 (MQ=255)                                                                                          ccTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCg                                            <  1:781554/140‑1 (MQ=255)                                                                                          ccTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCg                                            <  2:1028653/140‑1 (MQ=255)                                                                                            tgctgcATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTg                                          <  2:659713/140‑1 (MQ=255)                                                                                            tgctgcATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTg                                          <  2:757449/140‑1 (MQ=255)                                                                                             gctgcATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTgg                                         <  2:665669/140‑1 (MQ=255)                                                                                             gctgcATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTgg                                         <  1:6431/140‑1 (MQ=255)                                                                                                 cATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGAt                                     >  1:591780/1‑140 (MQ=255)                                                                                                    cTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATaca                                  <  1:292573/140‑1 (MQ=255)                                                                                                           tCACGTAAATCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGTGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACg                           >  1:851804/1‑140 (MQ=255)                                                                                                                  aaTCCAGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTccacc                    >  2:816617/1‑140 (MQ=255)                                                                                                                       aGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGtt               >  2:1024744/1‑140 (MQ=255)                                                                                                                       aGCGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGtt               >  2:708128/1‑140 (MQ=255)                                                                                                                         cGGCAGTGTTGGAAGAGAGGTGGTTTGTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGtttt             >  2:107033/1‑140 (MQ=255)                                                                                                                         cGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGaa                             >  2:163986/1‑124 (MQ=255)                                                                                                                         cGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGaa                             <  1:163986/124‑1 (MQ=255)                                                                                                                         cGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGtttt             >  2:881252/1‑140 (MQ=255)                                                                                                                         cGGCAGTGTTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGtttt             >  1:459531/1‑140 (MQ=255)                                                                                                                             aGTGTTGGAAGAGAGGTGGTTTCTGAAACGGTGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCaa         <  2:851804/140‑1 (MQ=255)                                                                                                                               tgtTGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAAtt       >  2:331295/1‑140 (MQ=255)                                                                                                                                 ttGGAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCa     <  1:284970/140‑1 (MQ=255)                                                                                                                                   ggAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACg   <  1:897720/140‑1 (MQ=255)                                                                                                                                    gAAGAGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGa  <  1:881252/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        GAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGA  >  NC_000913/1366965‑1367235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑